First report of bovine herpesvirus 1 isolation from bull semen samples in China

文献类型： 外文期刊

第一作者： Zhu, L.

作者： Zhu, L.;Yu, Y.;Jiang, X.;Zhu, G.;Zhu, L.;Yu, Y.;Jiang, X.;Zhu, G.;Yuan, W.

作者机构：

关键词： BoHV-1;virus isolation;VP16;CPE

期刊名称：ACTA VIROLOGICA （ 影响因子：1.162； 五年影响因子：1.255 ）

ISSN： 0001-723X

年卷期： 2017 年 61 卷 4 期

页码：

收录情况： SCI

摘要： Bovine herpesvirus 1 (BoHV-1) infection causes substantial economic losses to the cattle industry worldwide. So far, the isolation of BoHV-1 field virus has not been reported in China. Here, for the first time we report that two isolates of BoHV-1 designated as NJ16-1 and NJ16-2 were obtained from semen samples from breeding bulls in China. Typical cytopathic effect in MDBK cells, detection of viral protein VP16 in western blot analysis, PCR detection of BoHV-1 gB gene proved BoHV-1 infection and subsequent nucleotide sequence analysis showed a 99% identity with BoHV-1 Cooper strain. These results suggest that these isolated viruses are BoHV-1.

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