Metabolic profile of the masked mycotoxin T-2 toxin-3-glucoside in rats (in vitro and in vivo) and humans (in vitro)
文献类型: 外文期刊
第一作者: Yang, S.
作者: Yang, S.;Yang, S.;De Saeger, S.;De Boevre, M.;Yang, S.;Wang, Z.;Zhang, S.;Van Poucke, C.
作者机构:
关键词: T-2 toxin;T-2 toxin-3-glucoside;metabolism;liver;UHPLC-QTOF
期刊名称:WORLD MYCOTOXIN JOURNAL ( 影响因子:3.353; 五年影响因子:3.108 )
ISSN: 1875-0710
年卷期: 2017 年 10 卷 4 期
页码:
收录情况: SCI
摘要: The metabolic profile of T-2 toxin-3-glucoside (T2-Glc) in humans and rats was investigated using ultra performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UHPLC-QTOF). When rat and human liver microsomes were incubated with T2-Glc, a total of five metabolites were detected. T2-Glc exposed a higher metabolic stability in rats and human than T-2 toxin (T-2). The metabolism of T2-Glc by the intestinal microbiota of human and rats was also investigated, and three metabolites were observed. T2-Glc was reconverted to T-2 during incubation with fresh faeces. Furthermore, in vivo metabolism of T2-Glc in rats after oral administration was carried out, and three metabolites were detected in rat urine and faeces (T-2, HT-2 toxin and 3'-OH-T2-Glc). In vivo metabolism results indicated that T2-Glc was mainly metabolised in the gastro-intestinal tract with a low absorption level in rats. The results demonstrated that hydroxylation (C-3' and C-4'), hydrolysis (C-4 and C-8) and deconjugation are the main metabolic pathways of T2-Glc in mammals.
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