Functional characterization of (E)-beta-caryophyllene synthase from lima bean and its up-regulation by spider mites and alamethicin

文献类型： 外文期刊

第一作者： Fu Ning-ning

作者： Fu Ning-ning;Li Feng-qi;Wang Gui-rong;Zhou Jing-jiang

作者机构：

关键词： plant defence;ferpene synthases;(E)-beta-caryophyllene;lima bean

期刊名称：JOURNAL OF INTEGRATIVE AGRICULTURE （ 影响因子：2.848； 五年影响因子：2.979 ）

ISSN： 2095-3119

年卷期： 2017 年 16 卷 10 期

页码：

收录情况： SCI

摘要： (E)-beta-Caryophyllene is a sesquiterpene compound widely distributed in plants and functions in plant defence. However, little is known about the sequence and function of (E)-beta-caryophyllene synthase in lima bean (Phaseolus lunatus). Here, we report a new full-length cDNA (PICAHS) encoding (E)-beta-caryophyllene synthase, a possible key enzyme of plant defence. The cDNA of PICAHS contains an open reading frame of 1761 bp, encoding a protein of 586 amino acids with a predicted mass of 67.95 kDa. The deduced amino acid sequence shows 52% identity with sesquiterpene synthase MtCAHS of Medicago truncatula. Based on phylogenetic analysis, PICAHS is classified as the terpene synthases (TPS)-a subfamily. The recombinant enzyme, expressed in Escherichia coli, catalysed the formation of a major product (E)-beta-caryophyllene (82%) and a minor product a-humulene (18%) from farnesyl diphosphate. Real-time quantitative PCR (qRT-PCR) analysis found that the PICAHS transcript was significantly up-regulated in leaves after treatment with spider mites and alamethicin (ALA), suggesting its ecological function in plant defence.

分类号：

  • 相关文献

[1]Transcriptome Sequencing of Lima Bean (Phaseolus lunatus) to Identify Putative Positive Selection in Phaseolus and Legumes. Cao, Depan,Liu, Yang,Yang, Ting,Wang, Guirong. 2015

[2](E)-beta-caryophyllene functions as a host location signal for the rice white-backed planthopper Sogatella furcifera. Wang, Qi,Xin, Zhaojun,Li, Jiancai,Hu, Lingfei,Lou, Yonggen,Lu, Jing,Wang, Qi,Xin, Zhaojun.

[3]Expression regulation of a xylanase inhibitor gene riceXIP in rice (Oryza sativa L.). Zhan, Yihua,Sun, Xiangyu,Xu, Ying,Huang, Yingying,Jiang, Dean,Weng, Xiaoyan,Sun, Renjie,Hou, Chunxiao,Hou, Chunxiao. 2017

[4]Identification of two transcription factors activating the expression of OsXIP in rice defence response. Zhan, Yihua,Sun, Xiangyu,Huang, Yingying,Jiang, Dean,Weng, Xiaoyan,Rong, Guozeng,Hou, Chunxiao,Hou, Chunxiao. 2017

[5]The whitefly-associated facultative symbiont Hamiltonella defensa suppresses induced plant defences in tomato. Su, Qi,Xie, Wen,Wu, Qingjun,Wang, Shaoli,Zhang, Youjun,Su, Qi,Oliver, Kerry M..

[6]Comparative transcriptional profiling of orange fruit in response to the biocontrol yeast Kloeckera apiculata and its active compounds. Liu, Pu,Li, Guofeng,Liu, Pu,Chen, Kai,Long, Chao-an,Yang, Xiaoping. 2016

[7]Plant defence negates pathogen manipulation of vector behaviour. Liu, Baiming,Shi, Xiaobin,Wu, Huaitong,Xie, Wen,Wang, Shaoli,Wu, Qingjun,Zhang, Youjun,Liu, Baiming,Preisser, Evan L.,Li, Chuanyou,Li, Chuanyou.

[8]The jasmonic acid-amino acid conjugates JA-Val and JA-Leu are involved in rice resistance to herbivores. Fu, Wenjie,Jin, Gaochen,Wang, Xinjue,Lou, Yonggen,Li, Ran,Jimenez-Aleman, Guillermo H.,Song, Jiajin,Li, Suhua.

作者其他论文 更多>>

微信小程序