Vitrification of Rhesus Macaque Mesenchymal Stem Cells and the Effects on Global Gene Expression

文献类型： 外文期刊

第一作者： Fu, Xufeng

作者： Fu, Xufeng;Yan, Yaping;Li, Shanshan;Jiang, Bin;Wang, Hong;Duan, Yanchao;Tan, Tao;Niu, Yuyu;Ji, Weizhi;Si, Wei;Fu, Xufeng;Zheng, Bingrong;Fu, Xufeng;Wang, Junfeng;Wang, Hong;Tan, Tao;Niu, Yuyu;Ji, Weizhi;Si, Wei;Tan, Tao;Niu, Yuyu;Ji, Weizhi;Si, Wei;Gao, Fei;Gong, Desheng

作者机构：

期刊名称：STEM CELLS INTERNATIONAL （ 影响因子：5.443； 五年影响因子：5.305 ）

ISSN： 1687-966X

年卷期： 2017 年

页码：

收录情况： SCI

摘要： Mesenchymal stem cells (MSCs) are one of the most promising adult stem cells for clinical application in a cell therapy. The development of large-scale cryopreservation techniques, such as vitrification, for MSCs is a prerequisite for clinical therapies. Dimethyl sulfoxide (DMSO) and ethylene glycol (EG) are two types of cryoprotectants widely used for cell vitrification. However, the effects of DMSO and EG on the biological characteristics and transcriptome profiles of MSCs after cryopreservation remain unknown. In the present study, the viability, immunophenotype of cell surface markers, proliferation, differentiation potency, and global gene expression of rhesus macaque bone marrow-derived MSCs vitrified using DMSO and EG were studied. The results showed that vitrification did not affect the morphology, surface markers, and differentiation of the MSCs, and compared to DMSO, EG better protected cell viability and proliferation. Most importantly, vitrification resulted in changes in a large number of transcripts of MSCs either preserved using DMSO or EG. This report is the first to examine the effects of DMSO and EG on global gene expression in stem cells. These results will be beneficial to understanding the biological process involved in MSC vitrification and will contribute to improving cryopreservation protocols that maintain transcriptomic identity with high cryosurvival for preclinical research and clinical long-term storage.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序