Effect of ploidy level on expression of lycopene biosynthesis genes and accumulation of phytohormones during watermelon (Citrullus lanatus) fruit development and ripening

文献类型: 外文期刊

第一作者: Dou Jun-ling

作者: Dou Jun-ling;Yuan Ping-li;Zhao Sheng-jie;He Nan;Zhu Hong-ju;Gao Lei;Ji Wan-li;Lu Xu-qiang;Liu Wen-ge;Dou Jun-ling;Zhu Hong-ju

作者机构:

关键词: watermelon;lycopene;gene expression;ploidy;flesh color;phytohormone

期刊名称:JOURNAL OF INTEGRATIVE AGRICULTURE ( 影响因子:2.848; 五年影响因子:2.979 )

ISSN: 2095-3119

年卷期: 2017 年 16 卷 9 期

页码:

收录情况: SCI

摘要: The difference between lycopene and phytohornnone levels among diploid, triploid and tetraploid plants of two watermelon cultivars during fruit growth and ripening was studied. The expression pattern of five genes (phytoene synthase (PSY1), phytoene desaturase (PDS), zeta-carotene desaturase (ZDS), carotenoid isomerase (CRT/SO), and lycopene beta-cyclase (LCYB)) was analyzed in details. In red-fleshed cultivar Mimei, lycopene content increased rapidly from 25 to 35 days after pollination (DAP), and then decreased at 40 DAP. Triploid and tetraploid fruit had higher levels of lycopene than diploid. Moreover, triploid tended to contain more lycopene than tetraploid during fruit growth and ripening stages. However, little amount of lycopene (0-2 mg kg(-1) fresh weight (FVV)) in yellow-fleshed cultivar Huangmei was found during all fruit development stages. In Mimei, transcript level of PSY1 was generally higher than the other four genes, and LCYB gene expression was the lowest among all five genes being tested. PSY1, CRTISO and LCYB genes showed higher transcript levels in polyploid than in diploid fruit. By contrast, in Huangmei, transcript level of LCYB was not the lowest, but only lower than that of PSY1. PSY1, CRTISO and LCYB genes showed higher expression levels in diploid than in polyploid fruit. In Mimei, the negative correlation between gibberellane (GA) content and lycopene accumulation was determined in all three different ploidy fruits, while a positive correlation was observed between abscisic acid (ABA) content and lycopene accumulation only in diploid watermelon. These results indicated that different lycopene contents in different ploidy watermelons is regulated by the differential transcription expression of the lycopene metabolic genes and phytohormones.

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