Screening of genes of secreting acetic acid from Aspergillus niger H1 to improve phosphate solubilization

文献类型： 外文期刊

第一作者： Liu Xue

作者： Liu Xue;Zhu Chang-xiong;Liu Xue;Delvigne, Frank;Gong Ming-bo

作者机构：

关键词： phosphate solubilisation;Aspergillus niger;acetic acid

期刊名称：JOURNAL OF INTEGRATIVE AGRICULTURE （ 影响因子：2.848； 五年影响因子：2.979 ）

ISSN： 2095-3119

年卷期： 2017 年 16 卷 9 期

页码：

收录情况： SCI

摘要： A primary cDNA library of Aspergillus niger H1 was constructed using the switching mechanism at the 5' end of the RNA transcript (SMART) technique. A total of 169 clones exhibited halos when grown on tricalcium phosphate medium, and the H-46 clone displayed a clear halo. The full-length cDNA of the clone H-46 clone was 1 407 bp in length with a complete open reading frame (ORF) of 816 bp, and it encoded a protein that contained 272 amino acids. Multiple alignment analysis revealed a high degree of homology between the ORFs of the H-46 clone and the Bax inhibitor family (BI-1-like) proteins of other fungi. Acetic acid was secreted by Escherichia coli DH5 alpha that express the BI-1-like gene. The level attained was 492.52 mg L-1, which was associated with the release of 0.212 mg mL(-1) of soluble phosphate at 28 h. These results showed that the heterologous expression of BI-1-like genes in Eschericha coli DH5 alpha increased the secretion of acetic acid by altering the membrane permeability and enhancing the solubility of phosphate (P).

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