Penicillin-resistant characterization of Staphylococcus aureus isolated from bovine mastitis in Gansu, China
文献类型: 外文期刊
第一作者: Yang Feng
作者: Yang Feng;Liu Long-Hai;Wang Ling;Wang Xu-rong;Li Xin-Pu;Jin-yin, Luo;Zhang Zhe;Zhang Shi-dong;Yan Zuo-ting;Li Hong-sheng
作者机构:
关键词: Staphylococcus aureus;penicillin-resistant;beta-lactamase;biofilm;ica
期刊名称:JOURNAL OF INTEGRATIVE AGRICULTURE ( 影响因子:2.848; 五年影响因子:2.979 )
ISSN: 2095-3119
年卷期: 2017 年 16 卷 8 期
页码:
收录情况: SCI
摘要: Bovine mastitis caused by Staphylococcus aureus is difficult to treat because of increasing resistance against antibiotics, especially penicillin. beta-Lactamase and biofilm are responsible for penicillin resistance of S. aureus. The aim of this study was to investigate the beta-lactamase activity and biofilm formation capacity of 37 penicillin-resistant S. aureus strains (35 were blaZ positive and 2 were blaZ negative) from bovine mastitis in Gansu Province, China, as well as to measure the intercellular adhesion genes icaA and icaD of these strains. beta-Lactamase Test Kit was used to determine the beta-lactamase activity, biofilm formation was tested by semi-quantitative adherence assay method. Moreover, the presence of icaA and icaD were measured by PCR. A total of 32 penicillin-resistant S. aureus strains, including the two blaZ-negative strains, were identified as beta-lactamase producers. All tested S. aureus isolates produced biofilm in the microtiter plate assay. Meanwhile, all these strains were PCR-positive for the ica locus, icaA and icaD. The study indicated high prevalence of beta-lactamase activity, biofilm-forming capacity, and the ica genes among the penicillin-resistant S. aureus isolates, and implied that S. aureus resistant to penicillin was attributed to multiple mechanisms.
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