Resistance to Chronic Toxoplasma gondii Infection Induced by a DNA Vaccine Expressing GRA16

文献类型： 外文期刊

第一作者： Hu, Ling-Ying

作者： Hu, Ling-Ying;Zhang, Nian-Zhang;Zhang, Fu-Kai;Wang, Meng;Gao, Qi;Wang, Jin-Lei;Zhu, Xing-Quan;Hu, Ling-Ying;Gao, Qi;Zhu, Xing-Quan

作者机构：

期刊名称：BIOMED RESEARCH INTERNATIONAL （ 影响因子：3.411； 五年影响因子：3.62 ）

ISSN： 2314-6133

年卷期： 2017 年

页码：

收录情况： SCI

摘要： Toxoplasma gondii can infect all warm-blooded animals including human beings. T. gondii dense granule protein 16 (TgGRA16) as a crucial virulence factor could modulate the host gene expression. Here, a DNA vaccine expressing TgGRA16 was constructed to explore the protective efficacy against T. gondii infection in Kunming mice. The immune responses induced by pVAX-GRA16 were also evaluated. Mice immunized with pVAX-GRA16 could elicit higher levels of specific IgG antibody and strong cellular response compared to those in controls. The DNA vaccination significantly increased the levels of cytokines (IFN-gamma, IL-2, IL-4, and IL-10) and the percentages of CD4+ and CD8+ T cells in mice. After lethal challenge, mice immunized with pVAX-GRA16 (8.4 +/- 0.78 days) did not show a significant longer survival time than that in controls (7.1 +/- 0.30 days) (P > 0.05). However, in chronic toxoplasmosis model (administration of 10 brain cysts of PRU strain orally), numbers of tissue cysts in mice immunized with pVAX-GRA16 were significantly reduced compared to those in controls (P < 0.05) and the rate of reduction could reach 43.89%. The results indicated that the TgGRA16 would be a promising vaccine candidate for further development of effective epitope-based vaccines against chronic T. gondii infection in mice.

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