Molecular cloning and characterization of High Mobility Group box (HMGB) gene from Beauveria bassiana- infected silkworm, Bombyx mori

文献类型： 外文期刊

第一作者： Chengxiang, H.

作者： Chengxiang, H.;Han, W.;Dingding, L.;Ruilin, L.;Xijie, G.;Chengxiang, H.;Han, W.;Dingding, L.;Ruilin, L.;Xijie, G.

作者机构：

关键词： Bombyx mori;Beauveria bassiana;High Mobility Group box protein;rapid amplification of cDNA ends;qRT-PCR

期刊名称：ISJ-INVERTEBRATE SURVIVAL JOURNAL （ 影响因子：1.115； 五年影响因子：1.254 ）

ISSN： 1824-307X

年卷期： 2017 年 14 卷

页码：

收录情况： SCI

摘要： The cDNA sequence of High Mobility Group box ( HMGB) from Bombyx mori was cloned by rapid amplification of cDNA ends and submitted to GenBank ( the accession number was JF969272). The full-length cDNA of BmHMGB included 3015 bp, with five exons and four introns. It contained a 313 bp 5' UTR, a 2114 bp 3' UTR with a polyadenylation signal sequence AATAAA and a poly( A) tail. It encodes a 195- amino acid polypeptide with a predicted molecular weight about 22991.9Da and a theoretical isoelectric point 10.00. Phylogenetic analysis revealed that BmHMGB is grouped in insect HMGB proteins. BmHMGB was mainly expressed in hemolymph, cuticles, midgut and fat body by RT-PCR. In these tissues, the relative expression of BmHMGB with Beauveria bassiana infected silkworm was higher than the normal ones by fluorescent quantitative real-time PCR. Therefore, BmHMGB may play an important function in the response to B. bassiana infection of silkworms.

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