Molecular cloning and characterization of High Mobility Group box (HMGB) gene from Beauveria bassiana- infected silkworm, Bombyx mori

文献类型： 外文期刊

第一作者： Chengxiang, H.

作者： Chengxiang, H.;Han, W.;Dingding, L.;Ruilin, L.;Xijie, G.;Chengxiang, H.;Han, W.;Dingding, L.;Ruilin, L.;Xijie, G.

作者机构：

关键词： Bombyx mori;Beauveria bassiana;High Mobility Group box protein;rapid amplification of cDNA ends;qRT-PCR

期刊名称：ISJ-INVERTEBRATE SURVIVAL JOURNAL （ 影响因子：1.115； 五年影响因子：1.254 ）

ISSN： 1824-307X

年卷期： 2017 年 14 卷

页码：

收录情况： SCI

摘要： The cDNA sequence of High Mobility Group box ( HMGB) from Bombyx mori was cloned by rapid amplification of cDNA ends and submitted to GenBank ( the accession number was JF969272). The full-length cDNA of BmHMGB included 3015 bp, with five exons and four introns. It contained a 313 bp 5' UTR, a 2114 bp 3' UTR with a polyadenylation signal sequence AATAAA and a poly( A) tail. It encodes a 195- amino acid polypeptide with a predicted molecular weight about 22991.9Da and a theoretical isoelectric point 10.00. Phylogenetic analysis revealed that BmHMGB is grouped in insect HMGB proteins. BmHMGB was mainly expressed in hemolymph, cuticles, midgut and fat body by RT-PCR. In these tissues, the relative expression of BmHMGB with Beauveria bassiana infected silkworm was higher than the normal ones by fluorescent quantitative real-time PCR. Therefore, BmHMGB may play an important function in the response to B. bassiana infection of silkworms.

分类号：

  • 相关文献

[1]Molecular cloning and characterization of biphenyl hydrolase-like (BPHL) protein gene from silkworm, Bombyx mori. Gao, Kun,Deng, Xiang Yuan,Gao, Kun,Shang, Meng Ke,Qian, He Ying,Guo, Xi Jie,Gao, Kun,Shang, Meng Ke,Qian, He Ying,Guo, Xi Jie. 2016

[2]Cloning, characterization, and expression analysis of a novel BmGDAP1 gene from silkworm, Bombyx mori, involved in cytoplasmic polyhedrosis virus infection. Gao, Kun,Qian, Heying,Wu, Ping,Qin, Guangxing,Guo, Xijie,Gao, Kun,Qian, Heying,Wu, Ping,Qin, Guangxing,Guo, Xijie,Gao, Kun,Deng, Xiangyuan.

[3]Novel protein of IBP from silkworm, Bombyx mori, involved in cytoplasmic polyhedrosis virus infection. Gao, Kun,Qian, He-ying,Wu, Ping,Qin, Guang-xing,Liu, Ting,Shen, Zhong-yuan,Guo, Xi-jie,Gao, Kun,Qian, He-ying,Wu, Ping,Qin, Guang-xing,Liu, Ting,Shen, Zhong-yuan,Guo, Xi-jie,Gao, Kun,Deng, Xiang-yuan. 2012

[4]Cloning and characterization of the gene encoding an ubiquitin-activating enzyme E1 domain-containing protein of silkworm, Bombyx mori. Wu, Ping,Li, Mu-Wang,Guo, Xi-Jie,Jiang, Yun-Feng,Wang, Zi-Sheng,Guo, Xi-Jie. 2010

[5]Bombyx mori cecropin A has a high antifungal activity to entomopathogenic fungus Beauveria bassiana. Lu, Dingding,Geng, Tao,Hou, Chengxiang,Huang, Yuxia,Qin, Guangxing,Guo, Xijie,Lu, Dingding,Geng, Tao,Hou, Chengxiang,Huang, Yuxia,Qin, Guangxing,Guo, Xijie,Lu, Dingding.

[6]Expression profiling of Bombyx mori gloverin2 gene and its synergistic antifungal effect with cecropin A against Beauveria bassiana. Lu, Dingding,Geng, Tao,Hou, Chengxiang,Qin, Guangxing,Gao, Kun,Guo, Xijie,Lu, Dingding,Geng, Tao,Hou, Chengxiang,Qin, Guangxing,Gao, Kun,Guo, Xijie,Lu, Dingding.

[7]Inductive expression patterns of genes related to Toll signaling pathway in silkworm (Bombyx mori) upon Beauveria bassiana infection. Geng, Tao,Huang, Yuxia,Hou, Chengxiang,Qin, Guangxing,Lv, Dingding,Guo, Xijie,Hou, Chengxiang,Qin, Guangxing,Guo, Xijie. 2016

[8]JAK/STAT signaling pathway-mediated immune response in silkworm (Bombyx mori) challenged by Beauveria bassiana. Geng, Tao,Lv, Ding-Ding,Huang, Yu-Xia,Hou, Cheng-Xiang,Qin, Guang-Xing,Guo, Xi-Jie,Hou, Cheng-Xiang,Qin, Guang-Xing,Guo, Xi-Jie.

[9]Cloning of Acyl-ACP thioesterase FatA from Arachis hypogaea L. and its expression in Escherichia coli. Chen, G.,Peng, Z. Y.,Xuan, N.,Zhang, Y.,Bi, Y. P.,Chen, G.. 2012

[10]Characterization of a TIR-NBS-LRR gene associated with downy mildew resistance in grape. Fan, J. J.,Xu, X.,Ruan, Y. Y.,Zhu, Y. S.,Cui, Z. H.,Zhang, L. J.,Wang, P.,Liu, K.. 2015

[11]Effects of Carbon Concentrations and Carbon to Nitrogen ratios on Sporulation of Two Biological Control Fungi as Determined by Different Culture Methods. Liu, Xingzhong,Gao, Li.

[12]Potential of a strain of Beauveria bassiana (Hypocreales: Cordycipitaceae) for the control of the potato tuberworm, Phthorimaea operculella (Zeller). Lei, Zhongren,Gao, Yulin,Rondon, Silvia I..

[13]Bioassay and Scanning Electron Microscopic Observations Reveal High Virulence of Entomopathogenic Fungus, Beauveria bassiana, on the Onion Maggot (Diptera: Anthomyiidae) Adults. Wu, Shengyong,Xing, Zhenlong,Lei, Zhongren,Wang, Xiaoqing,Lei, Zhongren.

[14]Effectiveness of Beauveria bassiana sensu lato strains for biological control against Rhipicephalus (Boophilus) microplus (Acari: Ixodidae) in China. Sun, Ming,Ren, Qiaoyun,Guan, Guiquan,Yin, Hong,Luo, Jianxun,Sun, Ming,Li, Yufeng,Ma, Chao,Han, Xueqing.

[15]Laboratory and greenhouse evaluation of a new entomopathogenic strain of Beauveria bassiana for control of the onion thrips Thrips tabaci. Gao, Yulin,Xu, Xuenong,Zhang, Yaping,Wang, Jing,Lei, Zhongren,Smagghe, Guy.

[16]Nematicidal activity of culture filtrate of Beauveria bassiana against Meloidogyne hapla. Liu, Ting,Duan, Yu-Xi,Wang, Xue,Liu, Ting,Wang, Li.

[17]Sublethal Effects of Beauveria bassiana (Ascomycota: Hypocreales) on Life Table Parameters of Frankliniella occidentalis (Thysanoptera: Thripidae). Wang, Haihong,Lei, Zhongren,Wang, Haihong.

[18]RNA-Seq Analyses for Two Silkworm Strains Reveals Insight into Their Susceptibility and Resistance to Beauveria bassiana Infection. Xing, Dongxu,Jiang, Liang,Xia, Qingyou,Xing, Dongxu,Yang, Qiong,Li, Qingrong,Xiao, Yang,Ye, Mingqiang. 2017

[19]Effects of Bacillus thuringiensis toxin Cry1Ac and Beauveria bassiana on Asiatic corn borer (Lepidoptera : Crambidae). Ma, Xiao-Mu,Liu, Xiao-Xia,Ning, Xia,Zhang, Bo,Han, Fei,Guan, Xiu-Min,Zhang, Qing-Wen,Tan, Yun-Feng. 2008

[20]Compatibility of Beauveria bassiana with Neoseiulus barkeri for Control of Frankliniella occidentalis. Gao Yu-lin,Xu Xue-nong,Lei Zhong-ren,Goettel, Mark S.. 2015

作者其他论文 更多>>

微信小程序