Klotho attenuated antibody-mediated porcine endothelial cell activation and injury

文献类型： 外文期刊

第一作者： Liu, Lu

作者： Liu, Lu;Gao, Hanchao;Hong, Chungu;He, Chen;Li, Zesong;Cai, Zhiming;Mou, Lisha;Liu, Lu;Gao, Hanchao;Pan, Dengke;Dai, Yifan;Hara, Hidetaka;Cooper, David K. C.

作者机构：

关键词： endothelial cell;klotho;xenoreactive antibodies;xenotransplantation

期刊名称：XENOTRANSPLANTATION （ 影响因子：3.907； 五年影响因子：3.948 ）

ISSN： 0908-665X

年卷期： 2017 年 24 卷 1 期

页码：

收录情况： SCI

摘要： Long-term success in pig-to-primate xenotransplantation is currently hampered by acute vascular rejection (AVR), characterized by endothelial cell (EC) activation and injury. Klotho has anti-apoptotic, anti-inflammatory effects on EC and protects EC against reactive oxygen species, rendering klotho a promising molecule to control AVR. In this study, porcine ECs were pre-incubated with klotho and then exposed to xenoreactive antibodies and complement. Real-time PCR revealed that klotho suppressed antibody-induced pro-inflammatory gene expression of VCAM-1 and IL-1 alpha. NF-kappa(B) activation, I kappa B alpha phosphorylation, was also attenuated by klotho administration. Furthermore, klotho induced in porcine EC resistance against complement-dependent cytotoxicity. Accompanying this change, the binding of IgG and IgM xenoreactive antibodies to porcine EC was decreased and the expression of anti--inflammatory gene HO-1 was upregulated. These findings indicated that klotho protein protected porcine EC from activation and injury caused by binding of xenoreactive antibodies and was a promising candidate molecule in a multitransgenic pig strategy for xenotransplantation.

分类号：

  • 相关文献

[1]Characterization of roles of SpaA in Erysipelothrix rhusiopathiae adhesion to porcine endothelial cells. Zhu, Weifeng,Cai, Chengzhi,Wang, Ya,Li, Jingtao,Kang, Chao,Sun, Xiaomei,Jin, Meilin,Zhu, Weifeng,Cai, Chengzhi,Wang, Ya,Li, Jingtao,Kang, Chao,Sun, Xiaomei,Jin, Meilin,Wu, Chao,Sun, Xiaomei,Jin, Meilin,Wu, Chao,Sun, Xiaomei,Jin, Meilin,Zhu, Weifeng. 2017

[2]Production of alpha 1,3-galactosyltransferase and cytidine monophosphate-N-acetylneuraminic acid hydroxylase gene double-deficient pigs by CRISPR/Cas9 and handmade cloning. Gao, Hanchao,Zhao, Chengjiang,Zhao, Yanli,Li, Zesong,Cai, Zhiming,Mou, Lisha,Gao, Hanchao,Zhao, Chengjiang,Xiang, Xi,Li, Yong,Pan, Dengke,Dai, Yifan,Hara, Hidetaka,Cooper, David K. C..

[3]Pig BMSCs Transfected with Human TFPI Combat Species Incompatibility and Regulate the Human TF Pathway in Vitro and in a Rodent Model. Ji, Hongchen,Li, Xiao,Yue, Shuqiang,Zhang, Zhuochao,Ma, Ben,Wang, Jing,Pu, Meng,Wang, Desheng,Duan, Juanli,Tao, Kaishan,Dou, Kefeng,Li, Junjie,Chen, Hui,Zhou, Liang,Feng, Chong,Pan, Dengke. 2015

[4]Endothelial Cells Regulate Cardiac Myocyte Reorganisation Through beta 1-Integrin Signalling. Zhang, Yu,Wei, Renyue,Ma, Jing,Zhao, Yanhua,Liu, Zhonghua,Li, Hai,Zhang, Yu,Li, Hai,Lian, Zhengxing. 2015

[5]Distribution Characteristics of Alleles of Classical SLA-I and II Genes and Bioinformatic Analysis of Novel Alleles in Guizhou Miniature Pigs. Chen, Jiajia,Liu, Zizhan,Wang, Zaigui,Chen, Jiajia,Liu, Zizhan,Yang, Shulin,Li, Kui. 2014

[6]2, 3, 7, 8-Tetrachlorodibenzo-p-dioxin promotes endothelial cell apoptosis through activation of EP3/p38MAPK/Bcl-2 pathway. Yu, Yu,Liu, Qian,Guo, Shumin,Kong, Deping,Yu, Ying,Yu, Yu,Liu, Qian,Guo, Shumin,Zhang, Qianqian,Tang, Juan,Liu, Guizhu,Kong, Deping,Li, Juanjuan,Yan, Shuai,Yu, Ying,Wang, Ruiguo,Wang, Peilong,Su, Xiaoou. 2017

[7]Generation of chimeric piglets by injection of embryonic germ cells from inbred Wuzhishan miniature pigs into blastocysts. Dong, Xiao,Mu, Yulian,Liu, Lixin,Chen, Hongping,Zhang, Li,Feng, Shutang,Dong, Xiao,Tsung, Hsiaochien,Mu, Yulian,Feng, Shutang,Wang, Hongjun. 2014

[8]Sulforaphane protects human umbilical vein cells against lipotoxicity by stimulating autophagy via an AMPK-mediated pathway. Wang, Ning,Zhao, Juyang,Liu, Qian,Kong, Baohua,Wang, Ning,Liu, Qian,Kong, Baohua,Diao, Xinping.

作者其他论文 更多>>

微信小程序