Creation of gene-specific rice mutants by AvrXa23-based TALENs

文献类型： 外文期刊

第一作者： Wang Fu-jun

作者： Wang Fu-jun;Wang Chun-lian;Zheng Chong-ke;Qin Teng-fei;Gao Ying;Zhao Kai-jun;Wang Fu-jun;Liu Pi-qing

作者机构：

关键词： TAL effector nucleases;AvrXa23;targeting;OsERF922;genome editing;rice

期刊名称：JOURNAL OF INTEGRATIVE AGRICULTURE （ 影响因子：2.848； 五年影响因子：2.979 ）

ISSN： 2095-3119

年卷期： 2017 年 16 卷 2 期

页码：

收录情况： SCI

摘要： Transcription activator-like effector (TALE) nucleases (TALENs) are increasingly used as a powerful tool for genome editing in a variety of organisms. We have previously cloned the TALE-coding gene avrXa23 from Xanthomonas oryzae pv. oryzae and developed an AvrXa23-based assembly system for designer TALEs or TALENs. Here, we exploit TALENs to induce mutagenesis of the rice ethylene response factor (ERF) transcription factor OsERF922 for testing the gene-editing efficiency of AvrXa23-based TALENs system. A pair of TALENs (T-KJ9/KJ10) was assembled and their nuclease activities were first confirmed in rice protoplast transient assay. The TALENs-expressing construct pT-KJ9/KJ10 was then used for rice transformation. We observed targeting somatic mutagenesis frequency of 15.0% in positive transgenic rice calli and obtained two mutant plants with nucleotide deletion or insertion at the designer target region. Our work demonstrates that the AvrXa23-based TALENs system can be used for site-specific genome editing in rice.

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