Mx Is Not Responsible for the Antiviral Activity of Interferon-alpha against Japanese Encephalitis Virus

文献类型： 外文期刊

第一作者： Zhou, Jing

作者： Zhou, Jing;Wang, Shi-Qi;Zhang, Xiao-Min;Gao, Zhi-Can;Chen, Pu-Yan;Zhou, Bin;Wei, Jian-Chao;Liu, Ke;Ma, Zhi-Yong

作者机构：

关键词： Mx1;Mx2;interferon-alpha (IFN alpha);Japanese encephalitis virus (JEV);antivirus;Brefeldin A (BFA)

期刊名称：VIRUSES-BASEL （ 影响因子：5.048； 五年影响因子：5.127 ）

ISSN： 1999-4915

年卷期： 2017 年 9 卷 1 期

页码：

收录情况： SCI

摘要： Mx proteins are interferon (IFN)-induced dynamin-like GTPases that are present in all vertebrates and inhibit the replication of myriad viruses. However, the role Mx proteins play in IFN-mediated suppression of Japanese encephalitis virus (JEV) infection is unknown. In this study, we set out to investigate the effects of Mx1 and Mx2 expression on the interferon-alpha (IFN alpha) restriction of JEV replication. To evaluate whether the inhibitory activity of IFN alpha on JEV is dependent on Mx1 or Mx2, we knocked down Mx1 or Mx2 with siRNA in IFN alpha-treated PK-15 cells and BHK-21 cells, then challenged them with JEV; the production of progeny virus was assessed by plaque assay, RT-qPCR, and Western blotting. Our results demonstrated that depletion of Mx1 or Mx2 did not affect JEV restriction imposed by IFN alpha, although these two proteins were knocked down 66% and 79%, respectively. Accordingly, expression of exogenous Mx1 or Mx2 did not change the inhibitory activity of IFN alpha to JEV. In addition, even though virus-induced membranes were damaged by Brefeldin A (BFA), overexpressing porcine Mx1 or Mx2 did not inhibit JEV proliferation. We found that BFA inhibited JEV replication, not maturation, suggesting that BFA could be developed into a novel antiviral reagent. Collectively, our findings demonstrate that IFN alpha inhibits JEV infection by Mx-independent pathways.

分类号：

  • 相关文献

[1]Flower-like ZnO nanostructure assisted loop-mediated isothermal amplification assay for detection of Japanese encephalitis virus. Ma, Yonghua,Guan, Guiquan,Luo, Jianxun,Niu, Qingli,Liu, Junlong,Yin, Hong,Liu, Guangyuan,Ma, Yonghua,Lu, Yan.

[2]Obtained transgenic wheat expressing pac1 mediated by Agrobacterium is resistant against Barley yellow dwarf virus-GPV. Yan Fei,Zheng Yinying,Zhang Wenwei,Xiao Hong,Li Shifang,Cheng Zhuomin.

[3]Rapid one-step construction of hairpin RNA. Yan, Pu,Shen, Wentao,Gao, Xinzheng,Zhou, Peng,Duan, Jun. 2009

[4]Poly(I:C) inhibits porcine reproductive and respiratory syndrome virus replication in MARC-145 cells via activation of IFIT3. Zhang, Lili,Liu, Jie,Bai, Juan,Wang, Xiaoye,Liu, Xing,Jiang, Ping,Du, Yijun. 2013

[5]Comparison of factors that may affect the inhibitory efficacy of transgenic RNAi targeting of baculoviral genes in silkworm, Bombyx mori. Jiang, Liang,Zhao, Ping,Wang, Genhong,Cheng, Tingcai,Jin, Shengkai,Lin, Ping,Xia, Qingyou,Jiang, Liang,Zhao, Ping,Wang, Genhong,Cheng, Tingcai,Jin, Shengkai,Lin, Ping,Sun, Qiang,Xia, Qingyou,Yang, Qiong,Xiao, Yang.

作者其他论文 更多>>

微信小程序