Joint acute and endocrine disruptive toxicities of malathion, cypermethrin and prochloraz to embryo-larval zebrafish, Darzio rerio
文献类型: 外文期刊
第一作者: Guo, Dongmei
作者: Guo, Dongmei;Wang, Yanhua;Qian, Yongzhong;Chen, Chen;Wang, Yanhua;Wang, Qiang;Jiao, Bining
作者机构:
关键词: Combined toxicity;Zebransh;Hypothalamic-pituitary-gonadal axis;Estrogen;Gene expression
期刊名称:CHEMOSPHERE ( 影响因子:7.086; 五年影响因子:6.956 )
ISSN: 0045-6535
年卷期: 2017 年 166 卷
页码:
收录情况: SCI
摘要: It remains a daunting challenge to determine ecotoxicological risks of exposure to mixtures of endocrine disrupting chemicals (EDCs) in environmental toxicology. In the present study, we investigated acute and endocrine disruptive toxicities of cypermethrin (CPM), malathion (MAL), prochloraz (PRO) and their binary mixtures of MAL + CPM and MAL + PRO to the early life stages of zebrafish. In the acute lethal toxicity test, three pesticides exhibited different levels of toxicity to zebrafish larvae, and the order of toxicity was as follows: CPM > PRO > MAL. The binary mixture of MAL + CPM displayed a synergistic effect on zebrafish larvae after exposure for 24, 48, 72 and 96 h. However, binary mixture of MAL + PRO showed an antagonistic effect. To evaluate the estrogenic effect, the expression of genes in the hypothalamic-pituitary-gonadal axis was assessed after zebrafish embryos were exposed to CPM, MAL, PRO and their binary mixtures from blastula stage (1 h post-fertilization,, 1 hpf) to 14 dpf (14 d post-fertilization). Our data indicated that the transcription patterns of many key genes (vtgl, vtg2, era, er beta 1, er beta 2, cyp19a1a and cypl9a1b) were affected in hatched zebrafish after exposure to CPM, MAL and PRO. Moreover, following exposure to binary mixtures of 1000 mu g/L MAL + 4 mu g/L CPM and 1000 mu g/L MAL + 900 mu g/L PRO, the gene expressions were significantly changed compared with the individual pesticides. Our data provided a better understanding of bidirectional interactions of toxic response induced by these pesticides. (C) 2016 Elsevier Ltd. All rights reserved.
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