A Competitive Bio-Barcode Amplification Immunoassay for Small Molecules Based on Nanoparticles

文献类型： 外文期刊

第一作者： Du, Pengfei

作者： Du, Pengfei;Jin, Maojun;Chen, Ge;Zhang, Chan;Jiang, Zejun;Zhang, Yanxin;Zou, Pan;She, Yongxin;Jin, Fen;Shao, Hua;Wang, Shanshan;Zheng, Lufei;Wang, Jing

作者机构：

期刊名称：SCIENTIFIC REPORTS （ 影响因子：4.379； 五年影响因子：5.133 ）

ISSN： 2045-2322

年卷期： 2016 年 6 卷

页码：

收录情况： SCI

摘要： A novel detection method of small molecules, competitive bio-barcode amplification immunoassay, was developed and described in this report. Through the gold nanoparticles (AuNPs) probe and magnetic nanoparticles (MNPs) probe we prepared, only one monoclonal antibody can be used to detect small molecules. The competitive bio-barcode amplification immunoassay overcomes the obstacle that the bio-barcode assay cannot be used in small molecular detection, as two antibodies are unable to combine to one small molecule due to its small molecular structure. The small molecular compounds, triazophos, were selected as targets for the competitive bio-barcode amplification immunoassay. The linear range of detection was from 0.04 ng mL(-1) to 10 ng mL(-1), and the limit of detection (LOD) was 0.02 ng mL(-1), which was 10-20 folds lower than ELISA (Enzyme Linked Immunosorbent Assay). A practical application of the proposed immunoassay was evaluated by detecting triazophos in real samples. The recovery rate ranged from 72.5% to 110.5%, and the RSD was less than 20%. These results were validated by GC-MS, which indicated that this convenient and sensitive method has great potential for small molecular in real samples.

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