QuEChERS Purification Combined with Ultrahigh-Performance Liquid Chromatography Tandem Mass Spectrometry for Simultaneous Quantification of 25 Mycotoxins in Cereals
文献类型: 外文期刊
第一作者: Sun, Juan
作者: Sun, Juan;Li, Weixi;Zhang, Yan;Hu, Xuexu;Wu, Li;Wang, Bujun;Sun, Juan;Li, Weixi;Zhang, Yan;Hu, Xuexu;Wu, Li;Wang, Bujun
作者机构:
关键词: ultrahigh performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS);QuEChERS;mycotoxin;cereals
期刊名称:TOXINS ( 影响因子:4.546; 五年影响因子:4.8 )
ISSN: 2072-6651
年卷期: 2016 年 8 卷 12 期
页码:
收录情况: SCI
摘要: A method based on the QuEChERS (quick, easy, cheap, effective, rugged, and safe) purification combined with ultrahigh performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS), was optimized for the simultaneous quantification of 25 mycotoxins in cereals. Samples were extracted with a solution containing 80% acetonitrile and 0.1% formic acid, and purified with QuEChERS before being separated by a C18 column. The mass spectrometry was conducted by using positive electrospray ionization (ESI+) and multiple reaction monitoring (MRM) models. The method gave good linear relations with regression coefficients ranging from 0.9950 to 0.9999. The detection limits ranged from 0.03 to 15.0 mu g.kg(-1), and the average recovery at three different concentrations ranged from 60.2% to 115.8%, with relative standard deviations (RSD%) varying from 0.7% to 19.6% for the 25 mycotoxins. The method is simple, rapid, accurate, and an improvement compared with the existing methods published so far.
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