Characterization of an Immunodominant Epitope in the Endodomain of the Coronavirus Membrane Protein

文献类型： 外文期刊

第一作者： Dong, Hui

作者： Dong, Hui;Zhang, Xin;Shi, Hongyan;Chen, Jianfei;Shi, Da;Zhu, Yunnuan;Feng, Li;Dong, Hui

作者机构：

关键词： immunodominant epitope;coronavirus;membrane protein;endodomain

期刊名称：VIRUSES-BASEL （ 影响因子：5.048； 五年影响因子：5.127 ）

ISSN： 1999-4915

年卷期： 2016 年 8 卷 12 期

页码：

收录情况： SCI

摘要： The coronavirus membrane (M) protein acts as a dominant immunogen and is a major player in virus assembly. In this study, we prepared two monoclonal antibodies (mAbs; 1C3 and 4C7) directed against the transmissible gastroenteritis virus (TGEV) M protein. The 1C3 and 4C7 mAbs both reacted with the native TGEV M protein in western blotting and immunofluorescence (IFA) assays. Two linear epitopes, 243YSTEART249 (1C3) and 243YSTEARTDNLSEQEKLLHMV262 (4C7), were identified in the endodomain of the TGEV M protein. The 1C3 mAb can be used for the detection of the TGEV M protein in different assays. An IFA method for the detection of TGEV M protein was optimized using mAb 1C3. Furthermore, the ability of the epitope identified in this study to stimulate antibody production was also evaluated. An immunodominant epitope in the TGEV membrane protein endodomain was identified. The results of this study have implications for further research on TGEV replication.

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