RNA Interference of the Ecdysone Receptor Genes EcR and USP in Grain Aphid (Sitobion avenae F.) Affects Its Survival and Fecundity upon Feeding on Wheat Plants

文献类型： 外文期刊

第一作者： Yan, Ting

作者： Yan, Ting;Chen, Hongmei;Sun, Yongwei;Yu, Xiudao;Xia, Lanqin

作者机构：

关键词： grain aphid (Sitobion avenae F.);ecdysone receptor (EcR);ultraspiracle protein (USP);RNA interference (RNAi)

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.923； 五年影响因子：6.132 ）

ISSN： 1422-0067

年卷期： 2016 年 17 卷 12 期

页码：

收录情况： SCI

摘要： RNA interference (RNAi) has been widely used in functional genomics of insects and received intensive attention in the development of RNAi-based plants for insect control. Ecdysone receptor (EcR) and ultraspiracle protein (USP) play important roles in molting, metamorphosis, and reproduction of insects. EcR and USP orthologs and their function in grain aphid (Sitobion avenae F.) have not been documented yet. Here, RT-PCR, qRT-PCR, dsRNA feeding assay and aphid bioassay were employed to isolate EcR and USP orthologs in grain aphid, investigate their expression patterns, and evaluate the effect of RNAi on aphid survival and fecundity, and its persistence. The results indicated that SaEcR and SaUSP exhibited similar expression profiles at different developmental stages. Oral administration of dsRNAs of SaEcR and dsSaUSP significantly decreased the survival of aphids due to the down-regulation of these two genes, respectively. The silencing effect was persistent and transgenerational, as demonstrated by the reduced survival and fecundity due to knock-down of SaEcR and SaUSP in both the surviving aphids and their offspring, even after switching to aphid-susceptible wheat plants. Taken together, our results demonstrate that SaEcR and SaUSP are essential genes in aphid growth and development, and could be used as RNAi targets for wheat aphid control.

分类号：

  • 相关文献

[1]Double-stranded RNA in the biological control of grain aphid (Sitobion avenae F.). Wang, Dahai,Sun, Yongwei,Wang, Hui,Xia, Lanqin,Wang, Dahai,Liu, Qi,Li, Xia.

[2]Identifying potential RNAi targets in grain aphid (Sitobion avenae F.) based on transcriptome profiling of its alimentary canal after feeding on wheat plants. Zhang, Min,Wang, Hui,Wang, Dahai,Ma, Youzhi,Xia, Lanqin,Zhou, Yuwen,Gao, Qiang,Jones, Huw Dylan. 2013

[3]Cloning, ligand-binding, and temporal expression of ecdysteroid receptors in the diamondback moth, Plutella xylostella. Tang, Baozhen,Dong, Wei,Liang, Pei,Gao, Xiwu,Dong, Wei,Zhou, Xuguo. 2012

[4]Expression profiles of the p38 MAPK signaling pathway from Chinese shrimp I Fenneropenaeus chinensis in response to viral and bacterial infections. He, Yuying,Liu, Ping,Li, Jian,Wang, Qingyin,He, Yuying,Li, Jian,Yao, Wanlong. 2018

[5]Effective inhibition of porcine transmissible gastroenteritis virus replication in ST cells by shRNAs targeting RNA-dependent RNA polymerase gene. Zhou, Jun-fang,Hua, Xiu-guo,Cui, Li,Zhu, Jian-guo,Miao, De-nian,Zou, Yong,He, Xi-zhong,Su, Wan-guo. 2007

[6]Double repression of soluble starch synthase genes SSIIa and SSIIIa in rice (Oryza sativa L.) uncovers interactive effects on the physicochemical properties of starch. Zhang, Guoyu,Cheng, Zhijun,Zhang, Xin,Guo, Xiuping,Su, Ning,Mao, Long,Wan, Jianmin,Zhang, Guoyu,Cheng, Zhijun,Zhang, Xin,Guo, Xiuping,Su, Ning,Mao, Long,Wan, Jianmin,Jiang, Ling,Wan, Jianmin.

[7]Knockdown of Microplitis mediator Odorant Receptor Involved in the Sensitive Detection of Two Chemicals. Ren, Li-Yan,Zhang, Yong-Jun,Wu, Kong-Ming,Guo, Yu-Yuan,Li, Ke-Ming. 2012

[8]Cloning and RNAi-mediated functional characterization of MaLac2 of the pine sawyer, Monochamus alternatus. Niu, B. -L.,Shen, W. -F.,Liu, Y.,Weng, H. -B.,He, L. -H.,Meng, Z. -Q.,Tao, Y. -Z.,Mu, J. -J.,Wu, Z. -L.,Jiang, P.. 2008

[9]Cross-inhibition to heterologous foot-and-mouth disease virus infection induced by RNA interference targeting the conserved regions of viral genome. Liu, MQ,Chen, WZ,Ni, Z,Yan, WY,Fei, L,Jiao, Y,Zhang, J,Du, QY,Wei, X,Chen, JL,Liu, YM,Zheng, ZX.

[10]Expression of hairpin RNA (hpRNA) targeting the three CTV-silencing suppressor genes confers sweet orange with stem-pitting CTV tolerance. Cheng, Chunzhen,Zhang, Yongyan,Cheng, Chunzhen,Zhong, Yun,Yang, Jiawei.

作者其他论文 更多>>

微信小程序