Proteomics Analyses and Morphological Structure of Bacillus subtilis Inactivated by Pulsed Magnetic Field
文献类型: 外文期刊
第一作者: Qian, Jingya
作者: Qian, Jingya;Zhou, Cunshan;Ma, Haile;Li, Shujun;Abdualrahman, Mohammed A. Y.;Qian, Jingya;Zhou, Cunshan;Ma, Haile;Qian, Jingya;Li, Shujun;Yagoub, Abu ElGasim A.;Abdualrahman, Mohammed A. Y.
作者机构:
关键词: Bacillus subtilis;Pulsed magnetic field;Proteomics analyses;Cell morphology
期刊名称:FOOD BIOPHYSICS ( 影响因子:3.114; 五年影响因子:3.407 )
ISSN: 1557-1858
年卷期: 2016 年 11 卷 4 期
页码:
收录情况: SCI
摘要: Pulsed magnetic field (PMF) technology has emerged as a non-thermal method for inhibition of spoilage microorganism in food. In this study, we evaluate the effect of PMF treatment on the inactivation of Bacillus subtilis. The mechanisms responsible for cell death were also studied using transmission electron microscopy (TEM) and proteome approaches. Results showed that the survival rate of B. subtilis generally decreased with an increase of pulse numbers at the intensity of 3.30 T. The observation of TEM showed damage in cell cytoplasm and cytoplasmic membrane after PMF treatment. Additionally, 18 differentially expressed protein spots were identified by two dimensional gel electrophoresis (2D-GE) and matrix-assisted laser desorption/ionization-time-of-flight/time-of-flight (MALDI-TOF/TOF) analysis. The down-regulated outer membrane protein A (OmpA) illustrated that PMF destroyed the cell membrane. Furthermore, Gene ontology (GO) analysis and kyoto encyclopedia of genes and genomes (KEGG) pathway analysis were used to characterize the functions of those proteins. That PMF treatment damaged the membrane component, depressed cellular molecular functions and biological process, and decreased the carbohydrate metabolism and energy metabolism, which explain the death of cells. The presented results give the better view into the proteome of food microorganism and provide insight into the nature of PMF inactivation mechanisms.
分类号:
- 相关文献
作者其他论文 更多>>
-
Mushroom alcohol(1-octen-3-ol)and other 7 aroma compounds selected from Chinese dry-cured hams can enhance saltiness perception
作者:Xiao, Zuobing;Liu, Longxue;Niu, Yunwei;Zhang, Jing;Xiao, Zuobing;Wang, Daoying;Zhou, Cunshan
关键词:Odor-induced saltiness enhancement; Chinese dry-cured hams; Key volatile compounds; Different concentration NaCl solution
-
Revealing the ACE receptor binding properties and interaction mechanisms of salty oligopeptides from Stropharia rugosoannulata mushroom by molecular simulation and antihypertensive evaluation
作者:Li, Wen;Chen, Wanchao;Zhang, Zhong;Wu, Di;Liu, Peng;Li, Zhengpeng;Yang, Yan;Li, Wen;Chen, Wanchao;Zhang, Zhong;Wu, Di;Liu, Peng;Li, Zhengpeng;Yang, Yan;Li, Wen;Ma, Haile;Wang, Jinbin
关键词:
-
Comparative Transcriptome Analysis of Candidate Genes Associated with Mycelia Growth from a He-Ne Laser with Pulsed Light Mutant of Phellinus igniarius (Agaricomycetes)
作者:Dong, Yating;Ma, Haile;Sun, Ling;He, Ronghai;Dong, Yating;Gan, Bingcheng;Zhang, Qin;Gong, Zongjun;Han, Xing;Duan, Xinlian;Yan, Junjie;Ye, Xiaofei
关键词:Phellinus igniarius; mutant; comparative transcriptome; higher mycelia production; medicinal mushrooms
-
Exploring the taste presentation and receptor perception mechanism of salty peptides from Stropharia rugosoannulata based on molecular dynamics and thermodynamics simulation
作者:Li, Wen;Chen, Wanchao;Zhang, Zhong;Wu, Di;Yan, Mengqiu;Yang, Yan;Li, Wen;Sun, Shuai;Ma, Haile;Li, Tingzhao;Li, Tingzhao
关键词:Salty peptide; Molecular docking; Biolayer interferometry; Isothermal titration calorimetry
-
Characterization of Fusarium solani Associated with Tobacco (Nicotiana tabacum) Root Rot in Henan, China
作者:Qiu, Rui;Li, Caihong;Zhang, Yingying;Li, Xiaojie;Li, Chengjun;Liu, Chang;Zhang, Mengdan;Bai, Jingke;Chen, Yuguo;Li, Shujun;Li, Fangfang
关键词:cross-pathogenicity; genetic diversity; molecular analysis; morphological characteristics; Nectria haematococca
-
Research Progress on Soil Security Assessment in Farmlands and Grasslands Based on Bibliometrics over the Last Four Decades
作者:Chen, Fan;Li, Shujun;Wang, Lili;Hao, Lingyi;An, Yi;Huo, Lili;Li, Yutong;Zhu, Xiaoyu;Zhu, Xiaoyu
关键词:soil security; farmland and grassland; evaluation system; ecosystem; sustainability
-
Complete genome sequence of a novel dsRNA virus from the phytopathogenic fungus Fusarium oxysporum
作者:Wang, Jing;Ni, Yunxia;Zhao, Hui;Liu, Xintao;Liu, Hongyan;Qiu, Rui;Li, Shujun
关键词: