Analysis of transcriptome in hickory (Carya cathayensis), and uncover the dynamics in the hormonal signaling pathway during graft process

文献类型: 外文期刊

第一作者: Qiu, Lingling

作者: Qiu, Lingling;Jiang, Bo;Fang, Jia;Shen, Yike;Fang, Zhongxiang;Kumar, Saravana R. M.;Yan, Daoliang;Zheng, Bingsong;Fang, Jia;Shen, Yike;Fang, Zhongxiang;Kumar, Saravana R. M.;Yan, Daoliang;Zheng, Bingsong;Yi, Keke;Shen, Chenjia

作者机构:

关键词: Auxin;Cytokinin;Graft;Hickory;Transcriptome

期刊名称:BMC GENOMICS ( 影响因子:3.969; 五年影响因子:4.478 )

ISSN: 1471-2164

年卷期: 2016 年 17 卷

页码:

收录情况: SCI

摘要: Background: Hickory (Carya cathayensis), a woody plant with high nutritional and economic value, is widely planted in China. Due to its long juvenile phase, grafting is a useful technique for large-scale cultivation of hickory. To reveal the molecular mechanism during the graft process, we sequenced the transcriptomes of graft union in hickory. Results: In our study, six RNA-seq libraries yielded a total of 83,676,860 clean short reads comprising 4.19 Gb of sequence data. A large number of differentially expressed genes (DEGs) at three time points during the graft process were identified. In detail, 777 DEGs in the 7 d vs 0 d (day after grafting) comparison were classified into 11 enriched Gene Ontology (GO) categories, and 262 DEGs in the 14 d vs 0 d comparison were classified into 15 enriched GO categories. Furthermore, an overview of the PPI network was constructed by these DEGs. In addition, 20 genes related to the auxin-and cytokinin-signaling pathways were identified, and some were validated by qRT-PCR analysis. Conclusions: Our comprehensive analysis provides basic information on the candidate genes and hormone signaling pathways involved in the graft process in hickory and other woody plants.

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