Nanoscale Electric Characteristics and Oriented Assembly of Halobacterium salinarum Membrane Revealed by Electric Force Microscopy

文献类型： 外文期刊

第一作者： Li, Denghua

作者： Li, Denghua;Xu, Shiwei;Li, Zhemin;Li, Denghua;Wang, Yibing;Du, Huiwen;Yang, Yanlian;Wang, Chen

作者机构：

关键词： electrostatic force microscopy (EFM);purple membrane (PM);surface potential;peptides;oriented assembly

期刊名称：NANOMATERIALS （ 影响因子：5.076； 五年影响因子：5.346 ）

ISSN： 2079-4991

年卷期： 2016 年 6 卷 11 期

页码：

收录情况： SCI

摘要： Purple membranes (PM) of the bacteria Halobacterium salinarum are a unique natural membrane where bacteriorhodopsin (BR) can convert photon energy and pump protons. Elucidating the electronic properties of biomembranes is critical for revealing biological mechanisms and developing new devices. We report here the electric properties of PMs studied by using multi-functional electric force microscopy (EFM) at the nanoscale. The topography, surface potential, and dielectric capacity of PMs were imaged and quantitatively measured in parallel. Two orientations of PMs were identified by EFM because of its high resolution in differentiating electrical characteristics. The extracellular (EC) sides were more negative than the cytoplasmic (CP) side by 8 mV. The direction of potential difference may facilitate movement of protons across the membrane and thus play important roles in proton pumping. Unlike the side-dependent surface potentials observed in PM, the EFM capacitive response was independent of the side and was measured to be at a dC/dz value of similar to 5.25 nF/m. Furthermore, by modification of PM with de novo peptides based on peptide-protein interaction, directional oriented PM assembly on silicon substrate was obtained for technical devices. This work develops a new method for studying membrane nanoelectronics and exploring the bioelectric application at the nanoscale.

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