Molecular characteristic and pathogenicity analysis of a novel multiple recombinant ALV-K strain

文献类型： 外文期刊

第一作者： Li, Xinyi

作者： Li, Xinyi;Ma, Meige;Chang, Fangfang;Muhammad, Farooque;Yu, Mengmeng;Ren, Chaoqi;Bao, Yuanling;Zhang, Zhuo;Liu, Aijing;Pan, Qing;Gao, Li;Qi, Xiaole;Li, Kai;Liu, Changjun;Zhang, Yanping;Cui, Hongyu;Wang, Xiaomei;Gao, Yulong;Yu, Yan;Wang, Xiaomei

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关键词： Avian leukosis; Avian leukosis virus subgroup K; Viral isolation; Molecular characterization; Pathogenicity

期刊名称：VETERINARY MICROBIOLOGY （ 影响因子：3.293； 五年影响因子：3.599 ）

ISSN： 0378-1135

年卷期： 2021 年 260 卷

页码：

收录情况： SCI

摘要： Avian leukosis virus (ALV) can induce various tumors and cause serious production problems. ALVs isolated from chickens were divided into six subgroups (A-J). In 2012, a strain of a putative novel subgroup of ALVs was isolated from Chinese native chickens in Jiangsu Province and named as ALV-K. In this study, three ALV-K strains (JS14LH01, JS13LH14, and JS15SG01) were isolated from chickens with suspected ALV infection in Jiangsu Province. Their complete genomes were amplified, sequenced, and analyzed systematically. The results showed that JS14LH01 and JS13LH14 were ALV-K and ALV-E recombinant strains. Whereas JS15SG01 is an ALV-K, ALVE, and ALV-J multiple recombinant strain containing the U3 region of ALV-J. The pathogenicity test of JS15SG01 revealed that, compared with previous ALV-K strains, the viremia and viral shedding level of JS15SG01-infected chickens were significantly increased, reaching 100 % and 59 %, respectively. More important, JS15SG01 induced significant proliferation of gliocytes in the cerebral cortex of infected chickens, accompanied by the neurotropic phenomenon. This is the first report about a multiple recombinant ALV-K strain that could invade and injure the brain tissue of chickens in China. Our findings enriched the epidemiologic data of ALV and helped to reveal the evolution of ALV strains prevalent in chicken fields.

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