A newly characterized dense granule protein (GRA76) is important for the growth and virulence of Toxoplasma gondii

文献类型： 外文期刊

第一作者： Zheng, Xiao-Nan

作者： Zheng, Xiao-Nan;Sun, Li-Xiu;Li, Ting -Ting;Gao, Jin;Wu, Xiao-Jing;Zhang, Zhi-Wei;Wang, Meng;Fu, Bao-Quan;Wang, Jin -Lei;Zheng, Xiao-Nan;Gao, Jin;Wu, Xiao-Jing;Zhu, Xing-Quan;Sun, Li-Xiu;Elsheikha, Hany M.;Li, Ting -Ting;Wang, Meng;Fu, Bao-Quan;Wang, Jin -Lei

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关键词： Dense granule proteins; GRA76; Replication; Virulence; Bradyzoite differentiation; Toxoplasma gondii; Vaccine

期刊名称：INTERNATIONAL JOURNAL FOR PARASITOLOGY （ 影响因子：4.0； 五年影响因子：3.8 ）

ISSN： 0020-7519

年卷期： 2024 年 54 卷 2 期

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收录情况： SCI

摘要： Pathogenicity of the zoonotic pathogen Toxoplasma gondii largely depends on the secretion of effector proteins into the extracellular milieu and host cell cytosol, including the dense granule proteins (GRAs). The protein-encoding gene TGME49_299780 was previously identified as a contributor to parasite fitness. However, its involvement in parasite growth, virulence and infectivity in vitro and in vivo remains unknown. Here, we comprehensively examined the role of this new protein, termed GRA76, in parasite pathogenicity. Subcellular localization revealed high expression of GRA76 in tachyzoites inside the parasitophorous vacuole (PV). However, its expression was significantly decreased in bradyzoites. A CRISPR-Cas9 approach was used to knock out the gra76 gene in the T. gondii type I RH strain and type II Pru strain. The in vitro plaque assays and intracellular replication showed the involvement of GRA76 in replication of RH and Pru strains. Deletion of the gra76 gene significantly decreased parasite virulence, and reduced the brain cyst burden in mice. Using RNA sequencing, we detected a significant increase in the expression of bradyzoite-associated genes such as BAG1 and LDH2 in the PruDgra76 strain compared with the wild-type Pru strain. Using an in vitro bradyzoite differentiation assay, we showed that loss of GRA76 significantly increased the propensity for parasites to form bradyzoites. Immunization with PruDgra76 conferred partial protection against acute and chronic infection in mice. These findings show the important role of GRA76 in the pathogenesis of T. gondii and highlight the potential of PruDgra76 as a candidate for a live-attenuated vaccine. (c) 2023 Australian Society for Parasitology. Published by Elsevier Ltd. All rights reserved.

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