文献类型： 外文期刊
第一作者： Zhang, Shuang
作者： Zhang, Shuang;Zheng, Dong;Gao, Yuhua;Fan, Yanan;Bai, Chunyu;Fan, Yanan;Li, Lu;Guan, Weijun;Ma, Yuehui
作者机构：
期刊名称：CANADIAN JOURNAL OF VETERINARY RESEARCH-REVUE CANADIENNE DE RECHERCHE VETERINAIRE （ 影响因子：1.31； 五年影响因子：1.438 ）
ISSN： 0830-9000
年卷期： 2016 年 80 卷 4 期
页码：
收录情况： SCI
摘要： Adipose-derived stem cells (ADSCs) are multipotent, and can be differentiated into many cell types in vitro. In this study, tissues from pigs were chosen to identify and characterize ADSCs. Primary ADSCs were sub-cultured to passage 28. The surface markers of ADSCs: CD29, CD71, CD73, CD90, and CD166 were detected by reverse-transcription polymerase chain reaction assays and the markers CD29, CD44, CD105, and vimentin were detected by immunofluorescence. Growth curves and the capacity of clone-forming were performed to test the proliferation of ADSCs. Karyotype analysis showed that ADSCs cultured in vitro were genetically stable. To assess the differentiation capacity of the ADSCs, cells were induced to differentiate into osteoblasts, adipocytes, epithelial cells, neural cells, and hepatocyte-like cells. The results suggest that ADSCs from pigs showed similar biological characteristics with those separated from other species, and their multi-lineage differentiation shows potential as an application for cellular therapy in an animal model.
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