Phosphoproteome Analysis Reveals the Molecular Mechanisms Underlying Deoxynivalenol-Induced Intestinal Toxicity in IPEC-J2 Cells

文献类型: 外文期刊

第一作者: Zhang, Zhi-Qi

作者: Zhang, Zhi-Qi;Wang, Rui-Guo;Zhang, Wei;Wang, Pei-Long;Su, Xiao-Ou;Wang, Song-Bo;Wang, Song-Bo

作者机构:

关键词: deoxynivalenol;intestinal toxicity;phosphoproteomics;signaling pathways

期刊名称:TOXINS ( 影响因子:4.546; 五年影响因子:4.8 )

ISSN: 2072-6651

年卷期: 2016 年 8 卷 10 期

页码:

收录情况: SCI

摘要: Deoxynivalenol (DON) is a widespread trichothecene mycotoxin that commonly contaminates cereal crops and has various toxic effects in animals and humans. DON primarily targets the gastrointestinal tract, the first barrier against ingested food contaminants. In this study, an isobaric tag for relative and absolute quantitation (iTRAQ)-based phosphoproteomic approach was employed to elucidate the molecular mechanisms underlying DON-mediated intestinal toxicity in porcine epithelial cells (IPEC-J2) exposed to 20 mu M DON for 60 min. There were 4153 unique phosphopeptides, representing 389 phosphorylation sites, detected in 1821 phosphoproteins. We found that 289 phosphopeptides corresponding to 255 phosphoproteins were differentially phosphorylated in response to DON. Comprehensive Gene Ontology (GO) analysis combined with Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment revealed that, in addition to previously well-characterized mitogen-activated protein kinase (MAPK) signaling, DON exposure altered phosphatidylinositol 3-kinase/Akt (PI3K/Akt) and Janus kinase/signal transducer, and activator of transcription (JAK/STAT) pathways. These pathways are involved in a wide range of biological processes, including apoptosis, the intestinal barrier, intestinal inflammation, and the intestinal absorption of glucose. DON-induced changes are likely to contribute to the intestinal dysfunction. Overall, identification of relevant signaling pathways yielded new insights into the molecular mechanisms underlying DON-induced intestinal toxicity, and might help in the development of improved mechanism-based risk assessments in animals and humans.

分类号:

  • 相关文献

[1]Quantitative phosphoproteomic analysis among muscles of different color stability using tandem mass tag labeling. Li, Zheng,Li, Meng,Li, Xin,Xin, Jianzeng,Wang, Ying,Zhang, Dequan,Shen, Qingwu W.. 2018

[2]Phosphoproteomic analysis of the response of maize leaves to drought, heat and their combination stress. Hu, Xiuli,Wu, Liuji,Zhao, Feiyun,Li, Nana,Li, Chaohao,Wang, Wei,Zhang, Dayong,Zhu, Guohui. 2015

[3]Identification of putative phosphoproteins in wheat spikes induced by Fusarium graminearum. Ding, Lina,Cao, Jun,Zhou, Yang,Yang, Ruiying,Yang, Guoxing,Li, Peng.

[4]Transcriptional Profiling of Rice Treated with MoHrip1 Reveal the Function of Protein Elicitor in Enhancement of Disease Resistance and Plant Growth. Wang, Zhenzhen,Yang, Xiufen,Guo, Lihua,Qiu, Dewen,Zeng, Hongmei. 2016

[5]Expression analysis of major genes involved in signaling pathways during infection of Chinese cabbage with Hyaloperonospora brassicae. Gao, Tingting,Yu, Shuancang,Zhang, Fenglan,Yu, Yangjun,Zhang, Deshuang,Zhao, Xiuyun,Wang, Weihong,Gao, Tingting,Chen, Xuehao. 2014

[6]Recombinant TB9.8 of Mycobacterium bovis Triggers the Production of IL-12 p40 and IL-6 in RAW264.7 Macrophages via Activation of the p38, ERK, and NF-kappa B Signaling Pathways. Jia, Hong,Liu, Shuqing,Wu, Jing,Hou, Shaohua,Xin, Ting,Guo, Xiaoyu,Yuan, Weifeng,Zhang, Gaimei,Li, Ming,Zhu, Hongfei,Gao, Xintao,Qu, Hongfei,Zhu, Hongfei.

[7]Pre-infestation of Tomato Plants by Aphids Modulates Transmission-Acquisition Relationship among Whiteflies, Tomato Yellow Leaf Curl Virus (TYLCV) and Plants. Ge, Feng,Tan, Xiao L.,Chen, Ju L.,Tan, Xiao L.,Liu, Tong X.,Tan, Xiao L.,Liu, Tong X.,Benelli, Giovanni,Desneux, Nicolas,Yang, Xue Q.. 2017

[8]Simultaneously Testing Deoxynivalenol, T2 Toxin and Ochratoxin A in Wheat with a Colloidal Gold Test Strip and Matrix Solid-Phase Dispersion Cleaning. Jin, Nuo,Wang, Miao,Fan, Bei,He, Yan,Huang, Yatao,Wang, Fengzhong,Liu, Yang,Wang, Miao,Fan, Bei,He, Yan,Huang, Yatao,Wang, Fengzhong. 2017

[9]A minor survey of deoxynivalenol in Fusarium infected wheat from Yangtze-Huaihe river basin region in China. Cui, Li,Selvaraj, Jonathan Nimal,Xing, Fuguo,Zhao, Yueju,Zhou, Lu,Liu, Yang. 2013

[10]Relationship of Deoxynivalenol Content in Grain, Chaff, and Straw with Fusarium Head Blight Severity in Wheat Varieties with Various Levels of Resistance. Ji, Fang,Wu, Jirong,Zhao, Hongyan,Xu, Jianhong,Shi, Jianrong,Ji, Fang,Wu, Jirong,Zhao, Hongyan,Xu, Jianhong,Shi, Jianrong,Ji, Fang,Wu, Jirong,Zhao, Hongyan,Xu, Jianhong,Shi, Jianrong,Ji, Fang,Wu, Jirong,Zhao, Hongyan,Xu, Jianhong,Shi, Jianrong. 2015

[11]Simultaneous determination of deoxynivalenol, and 15-and 3-acetyldeoxynivalenol in cereals by HPLC-UV detection. Yang, D.,Geng, Z. M.,Yao, J. B.,Zhang, X.,Zhang, P. P.,Ma, H. X.. 2013

[12]The Antagonistic Effect of Mycotoxins Deoxynivalenol and Zearalenone on Metabolic Profiling in Serum and Liver of Mice. Ji, Jian,Cui, Fangchao,Pi, Fuwei,Zhang, Yinzhi,Li, Yun,Wang, Jiasheng,Sun, Xiulan,Zhu, Pei,Ji, Jian,Zhu, Pei,Cui, Fangchao,Pi, Fuwei,Zhang, Yinzhi,Sun, Xiulan,Li, Yun,Wang, Jiasheng. 2017

[13]Evaluation on levels and conversion profiles of DON, 3-ADON, and 15-ADON during bread making process. Wang, Bujun.

[14]Effects of feeding a Fusarium toxin-contaminated diet to infectious bursal disease virus-infected broilers on the protein turnover of the bursa of Fabricius and spleen. Daenicke, Sven,Pappritz, Julia,Goyarts, Tanja,Xu, Bu,Rautenschlein, Silke. 2011

[15]Effects of Adding Clostridium sp WJ06 on Intestinal Morphology and Microbial Diversity of Growing Pigs Fed with Natural Deoxynivalenol Contaminated Wheat. Li, FuChang,Huang, LiBo,Chen, HongJu,Wang, ChunYang,Wang, JinQuan. 2017

[16]Fates of deoxynivalenol and deoxynivalenol-3-glucoside during bread and noodle processing. Zhang, Huijie,Wang, Bujun,Zhang, Huijie,Wang, Bujun.

[17]Retention of deoxynivalenol and its derivatives during storage of wheat grain and flour. Zhang, Huijie,Sun, Juan,Zhang, Yan,Lu, Meibin,Sun, Lijuan,Li, Weixi,Hu, Xuexu,Wang, Bujun,Zhang, Huijie,Sun, Juan,Zhang, Yan,Lu, Meibin,Sun, Lijuan,Li, Weixi,Hu, Xuexu,Wang, Bujun.

[18]Occurrence of mycotoxins in feed ingredients and complete feeds obtained from the Beijing region of China. Zhao, Lihong,Fan, Yu,Ma, Shanshan,Ji, Cheng,Ma, Qiugang,Zhang, Jianyun,Jia, Yaxiong,Sun, Lei.

[19]Fate of deoxynivalenol and deoxynivalenol-3-glucoside during wheat milling and Chinese steamed bread processing. Zhang, Huijie,Wang, Bujun,Zhang, Huijie,Wang, Bujun. 2014

[20]Optimization for the Production of Deoxynivalenol and Zearalenone by Fusarium graminearum Using Response Surface Methodology. Wu, Li,Qiu, Lijuan,Zhang, Huijie,Sun, Juan,Hu, Xuexu,Wang, Bujun,Wu, Li,Zhang, Huijie,Sun, Juan,Hu, Xuexu,Wang, Bujun. 2017

作者其他论文 更多>>