iTRAQ-based quantitative proteomics reveals the biochemical mechanism of cold stress adaption of razor clam during controlled freezing-point storage

文献类型： 外文期刊

第一作者： Wang, Chong

作者： Wang, Chong;Chu, Jianjun;Fu, Linglin;Wang, Yanbo;Zhao, Feng;Zhou, Deqing

作者机构：

关键词： Razor clam;CFPS;Cold stress;iTRAQ;MAPK

期刊名称：FOOD CHEMISTRY （ 影响因子：7.514； 五年影响因子：7.516 ）

ISSN： 0308-8146

年卷期： 2018 年 247 卷

页码：

收录情况： SCI

摘要： Razor clam is a major cultivated shellfish of great economic importance and high nutritional value. Due to high corruptible potential, razor clam is generally preserved by controlled freezing-point storage (CFPS). Here, we applied isobaric tags for relative and absolute quantification (iTRAQ) labeling to investigate the biochemical mechanism of cold stress adaption in razor clam during CFPS. In total, 369 proteins were quantified, and 27 of them were identified as differentially expressed proteins during CFPS, mostly involved in energy metabolism process, DNA duplication and protein synthesis, and stress response, specifically, MAPK is the predominant pathway. Further qPCR results revealed H2A and S6K 2 alpha to be the critical post-transcriptionally regulated genes. Our results provided proteomics information with respect to the biochemical mechanism of cold stress adaption in razor clam, shed light on the further elongation of razor clams storage period, and help clarify the novel mechanisms of cold tolerance.

分类号：

  • 相关文献

[1]Identification of cold-stress responsive proteins in Anabasis aphylla seedlings via the iTRAQ proteomics technique. Wang, Tingting,Wang, Mei,Chu, Guangming,Ye, Chunxiu,Ye, Chunxiu. 2017

[2]Phylogenetic Analysis and Expression Patterns of the MAPK Gene Family in Wheat (Triticum aestivum L.). Lian Wei-wei,Tang Yi-miao,Gao Shi-qing,Zhang Zhao,Zhao Xin,Zhao Chang-ping,Lian Wei-wei. 2012

[3]The Transcription Factor AtDOF4.7 Is Involved in Ethylene- and IDA-Mediated Organ Abscission in Arabidopsis. Wang, Gao-Qi,Tan, Feng,Zhang, Xiao-Yan,Chen, Qi-Jun,Wang, Xue-Chen,Wei, Peng-Cheng,Yu, Man. 2016

[4]Stk2, a Mitogen-Activated Protein Kinase from Setosphaeria turcica, Specifically Complements the Functions of the Fus3 and Kss1 of Saccharomyces cerevisiae in Filamentation, Invasive Growth, and Mating Behavior. Gu Shou-qin,Yang Yang,Zhang Chang-zhi,Fan Yu,Zhang Xiao-yu,Tian Lan,Hao Zhi-min,Cao Zhi-yan,Gong Xiao-dong,Han Jian-min,Dong Jin-gao,Li Po,Fan Yong-shan. 2013

[5]Recombinant TB9.8 of Mycobacterium bovis Triggers the Production of IL-12 p40 and IL-6 in RAW264.7 Macrophages via Activation of the p38, ERK, and NF-kappa B Signaling Pathways. Jia, Hong,Liu, Shuqing,Wu, Jing,Hou, Shaohua,Xin, Ting,Guo, Xiaoyu,Yuan, Weifeng,Zhang, Gaimei,Li, Ming,Zhu, Hongfei,Gao, Xintao,Qu, Hongfei,Zhu, Hongfei.

[6]MAP kinase gene STK1 is required for hyphal, conidial, and appressorial development, toxin biosynthesis, pathogenicity, and hypertonic stress response in the plant pathogenic fungus Setosphaeria turcica. Li Po,Gong Xiao-dong,Jia Hui,Fan Yong-shan,Zhang Yun-feng,Han Jian-min,Gu Shou-qin,Dong Jin-gao,Li Po,Fan Yong-shan,Zhang Yun-feng. 2016

[7]The Antiviral Effects of Harmine Against BoHV-1 Infection In Vitro. Jiang, Xinyi,Zou, Jie,Zhuang, Yuncheng,Zhu, Liqian,Zhu, Guoqiang,Yuan, Weifeng.

[8]Mycoplasma hyopneumoniae induces pro-inflammatory cytokine and nitric oxide production through NF kappa B and MAPK pathways in RAW264.7 cells. Hwang, Mi-Hyun,Damte, Dereje,Lee, Joong-Su,Gebru, Elias,Chang, Zhi-Qiang,Rhee, Man-Hee,Park, Seung-Chun,Cheng, Henrique,Jung, Byeong-Yeal,Chang, Zhi-Qiang. 2011

[9]ABA plays essential roles in regulating root growth by interacting with auxin and MAPK signaling pathways and cell-cycle machinery in rice seedlings. Zhao, Feng Yun,Cai, Feng Xiang,Gao, Hua Jian,Wang, Kai,Liu, Tao,Wang, Xue,Zhang, Shi Yong.

[10]MAPKs as a cross point in H2O2 and auxin signaling under combined cadmium and zinc stress in rice roots. Zhao, F. Y.,Han, M. M.,Ren, J.,Hu, F.,Wang, X.,Zhang, S. Y..

[11]Expression analysis of genes encoding mitogen-activated protein kinases in maize provides a key link between abiotic stress signaling and plant reproduction. Sun, Wei,Sun, Hong Wei,Yang, Shu Ke,Lu, Xing Bo,Xu, Xiao Hui,Chen, Hao,Wang, Juan,Sang, Ya Lin,Chen, Hao,Sang, Ya Lin.

[12]MAPKs regulate root growth by influencing auxin signaling and cell cycle-related gene expression in cadmium-stressed rice. Zhao, Feng Yun,Hu, Fan,Wang, Kai,Zhang, Cheng Ren,Liu, Tao,Zhang, Shi Yong.

[13]Crosstalk between ABA, auxin, MAPK signaling, and the cell cycle in cadmium-stressed rice seedlings. Zhao, Feng Yun,Wang, Kai,Ren, Jing,Liu, Tao,Wang, Xue,Zhang, Shi Yong.

[14]NPR1-dependent salicylic acid signaling is not involved in elevated CO2-induced heat stress tolerance in Arabidopsis thaliana. Li, Xin,Ahammed, Golam Jalal,Li, Xin,Yu, Jingquan,Shi, Kai. 2015

[15]Enhanced Tolerance to Chilling Stress in Tomato by Overexpression of a Mitogen-Activated Protein Kinase, SlMPK7. Yu, Li,Yan, Jun,He, Lizhong,Zhu, Weimin,Yang, Yanjuan.

[16]Overexpression of tomato mitogen-activated protein kinase SlMPK3 in tobacco increases tolerance to low temperature stress. Yu, Li,Yan, Jun,Zhu, Weimin,Yang, Yanjuan.

[17]OsWRKY30 is activated by MAP kinases to confer drought tolerance in rice. Shen, Huaishun,Liu, Citao,Zhang, Yi,Meng, Xiuping,Wang, Xiping,Shen, Huaishun,Zhou, Xin,Liu, Citao,Chu, Chengcai,Liu, Citao,Chu, Chengcai.

[18]iTRAQ-proteomics and bioinformatics analyses of mammary tissue from cows with clinical mastitis due to natural infection with Staphylococci aureus. Huang, Jinming,Luo, Guojing,Zhang, Zijing,Wang, Xiuge,Ju, Zhihua,Qi, Chao,Zhang, Yan,Wang, Changfa,Li, Rongling,Li, Jianbin,Yin, Weijun,Zhong, Jifeng,Luo, Guojing,Zhang, Zijing,Xu, Yinxue,Moisa, Sonia J.,Loor, Juan J.,Loor, Juan J.,Moisa, Sonia J.,Loor, Juan J.. 2014

[19]Proteomic Analysis of Differences in Fiber Development between Wild and Cultivated Gossypium hirsutum L.. Yuan Qin,Yu, Shuxun,Hengling Wei,Huiru Sun,Pengbo Hao,Hantao Wang,Junji Su,Shuxun Yu.

[20]Comparative Proteomic Analysis of Gossypium thurberi in Response to Verticillium dahliae Inoculation. Weiping Fang,Deyi Xie,Heqin Zhu,Wu Li,Zhenzhen Xu,Lirong Yang,Zhifang Li,Li Sun,Jinxia Wang,Lihong Nie,Zhongjie Tang,Shuping Lv,Fu’an Zhao,Yao Sun,Yuanming Zhao,Jianan Hou,Xiaojie Yang. 2015

作者其他论文 更多>>

微信小程序