Injury and mechanism of recombinant E-coli expressing STa on piglets colon

文献类型： 外文期刊

第一作者： Lv, Yang

作者： Lv, Yang;Li, Xueni;Shi, Yutao;Du, Linxiao;Ding, Binying;Hou, Yongqing;Gong, Joshua;Wu, Tao;Zhang, Lin;Gong, Joshua

作者机构：

关键词： colon;heat-stable enterotoxin A;piglet;recombinant escherichia coli

期刊名称：JOURNAL OF VETERINARY MEDICAL SCIENCE （ 影响因子：1.267； 五年影响因子：1.348 ）

ISSN： 0916-7250

年卷期： 2018 年 80 卷 2 期

页码：

收录情况： SCI

摘要： Enterotoxigenic Escherichia coli (ETEC) is primary pathogenic bacteria of piglet diarrhea, over two thirds of piglets diarrhea caused by ETEC are resulted from STa-producing ETEC strains. This experiment was conducted to construct the recombinant E. coli expressing STa and study the injury and mechanism of recombinant E. coli expressing STa on 7 days old piglets colon. Twenty-four 7 days old piglets were allotted to four treatments: control group, STa group (2 x 10(9) CFU E. coli LMG194-STa), LMG194 group (2 x 10(9) CFU E. coli LMG194) and K88 group (2 x 10(9) CFU E. coli K88). The result showed that E. coli infection significantly increased diarrhea rates; changed DAO activity in plasma and colon; damaged colonic mucosal morphology including crypt depth, number of globet cells, density of lymphocytes and lamina propria cell density; substantially reduced antioxidant capacity by altering activities of GSH-Px, SOD, and TNOS and productions of MDA and H2O2; obviously decreased AQP3, AQP4 and KCNJ13 protein expression levels; substantially altered the gene expression levels of inflammatory cytokines. Conclusively, STa group had the biggest effect on these indices in four treatment groups. These results suggested that the recombinant strain expressed STa can induce piglets diarrhea and colonic morphological and funtional damage by altering expression of proteins connect to transportation function and genes associated with intestinal injury and inflammatory cytokines.

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