Toll-receptor 9 gene in the black tiger shrimp (Penaeus monodon) induced the activation of the TLR NF-kappa B signaling pathway

文献类型： 外文期刊

第一作者： Liu, Qian

作者： Liu, Qian;Jiang, Shigui;Huang, Jianhua;Zhou, Falin;Yang, Qibin;Jiang, Song;Yang, Lishi;Liu, Qian;Xu, Dan;Liu, Qian;Jiang, Shigui

作者机构：

关键词： Invertebrates;Innate immunity;Cell immune-model;LRRs-deletion mutants;Subcellular localization

期刊名称：GENE （ 影响因子：3.688； 五年影响因子：3.329 ）

ISSN： 0378-1119

年卷期： 2018 年 639 卷

页码：

收录情况： SCI

摘要： Toll receptors are important pathogen recognition receptors (PRRs) in shrimps, which play a vital role in defending against virus and bacterial challenge. In this paper, the characterization and functional analysis of a Toll9 receptor gene from Penaeus monodon was performed in HEK293T cells. Data showed that PmToll9 can activate the NF-kappa B promoter activities of TLR pathway, while ISRE and IFN-beta promoter cannot be activated obviously in HEK293T cells using dual-luciferase reporter system. The downstream immune factors of IL-8, I kappa Ba, and TRAF6 were activated by PmToll9 and IL-8 showed the most significant up-regulation in expression levels, indicating the activities of NF-KB can be mediated by PmToll9. Six LRRs-deletion mutants were constructed and results showed these mutants had obvious declines in luciferase activities, among which the mutant pCMV-DeLRR4 showed the most significant decline. qPCR data indicated LRRs-deletion mutants efficiently impaired the activities of the downstream immune factors IL-8, I kappa B-alpha, and TRAF6. It demonstrates that LRRs-deletion mutants could result in the weaken abilities of PmToll9 in signaling transduction. Overexpression of PmToll9-GFP fusion protein in Hela cells revealed the primary cellular localization of PmToll9 is in the cytoplasm.

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