Characterization and expression analysis of a chitinase gene (PmChi-5) from black tiger shrimp (Penaeus monodon) under pathogens infection and ambient ammonia-N stress

文献类型: 外文期刊

第一作者: Zhou, Falin

作者: Zhou, Falin;Zhou, Kaimin;Huang, Jianhua;Yang, Qibin;Jiang, Song;Qiu, Lihua;Yang, Lishi;Jiang, Shigui

作者机构:

关键词: Penaeus monodon;Chitinase;Streptococcus agalactiae;Vthrio harveyi;Ammonia nitrogen stress

期刊名称:FISH & SHELLFISH IMMUNOLOGY ( 影响因子:4.581; 五年影响因子:4.851 )

ISSN: 1050-4648

年卷期: 2018 年 72 卷

页码:

收录情况: SCI

摘要: Chitinases are crucial enzymes for crustaceans. Previous researches had already revealed that chitinases play important roles in digestion, molting and defense against viruses. In the present study, a chitinase cDNA was identified from black tiger shrimp (Penaeus monodon) and designated as PmChi-5. The full-length PmChi-5 cDNA was 2860 bp in size, containing an open reading frame (ORF) of 1731 bp that encoded a protein of 576 amino acids with a deduced molecular weight of 64.8 kDa. Expression of the PmChi-5 mRNA was ubiquitously detected in all selected tissues, with the highest level in the gill and hepatopancreas. PmChi-5 was expressed throughout the whole larvae stages, and the highest level at Mysis3 stage, which indicated that PmChi-5 may be involved in larval metamorphosis. After challenged with Streptococcus agalactiae and Vibrio harveyi, the transcripts of PmChi-5 were found to be up-regulated significantly both in hepatopancreas and gill. Besides, the ammonia nitrogen stress treatment was also carried out, PmChi-5 transcripts were significantly changed in hepatopancreas and gill. The results showed that PmChi-5 may be involved in molting, larval metamorphosis, the immune defenses to pathogens infection and ammonia-N stress.

分类号:

  • 相关文献

[1]Immunogenicity of the LrrG protein encapsulated in PLGA microparticles in Nile tilapia (Oreochromis niloticus) vaccinated against Streptococcus agalactiae. Ke, Xiaoli,Chen, Xue,Liu, Zhigang,Lu, Maixin,Gao, Fengying,Cao, Jianmeng,Chen, Xue. 2017

[2]Identification of a virulence-related surface protein XF in piscine Streptococcus agalactiae by pre-absorbed immunoproteomics. Liu, Guangjin,Zhang, Wei,Liu, Yongjie,Yao, Huochun,Lu, Chengping,Xu, Pao. 2014

[3]Multiple Evolutionary Selections Involved in Synonymous Codon Usages in the Streptococcus agalactiae Genome. Ma, Yan-Ping,Li, Yu-Gu,Ma, Yan-Ping,Ke, Hao,Liang, Zhi-Ling,Liu, Zhen-Xing,Hao, Le,Ma, Jiang-Yao. 2016

[4]Comparative proteome analysis of two Streptococcus agalactiae strains from cultured tilapia with different virulence. Li, Wei,Su, You-Lu,Mai, Yong-Zhan,Li, Yan-Wei,Li, An-Xing,Su, You-Lu,Mo, Ze-Quan.

[5]Isolation and characterization of Toll-like receptor 21 and 22 genes from Nile tilapia, Oreochromis niloticus (Linnaeus). Pang, Ji-cai,Gao, Feng-ying,Zhao, Jin-liang,Pang, Ji-cai,Gao, Feng-ying,Wang, Miao,Lu, Mai-xin,Pang, Ji-cai,Gao, Feng-ying,Wang, Miao,Lu, Mai-xin.

[6]Molecular characterization of Streptococcus agalactiae in diseased farmed tilapia in China. Zhang, Defeng,Li, Aihua,Zhang, Qianqian,Gong, Xiaoning,Zhang, Defeng,Zhang, Qianqian,Guo, Yujuan,Chen, Xuenian,Zhang, Defeng. 2013

[7]luxS/AI-2 Quorum Sensing Is Involved in Antimicrobial Susceptibility in Streptococcus agalactiae. Ma, Yan Ping,Ke, Hao,Hao, Le,Liu, Zhen Xing,Liang, Zhi Ling,Ma, Jiang Yao,Ma, Yan Ping,Ke, Hao,Hao, Le,Liu, Zhen Xing,Liang, Zhi Ling,Ma, Jiang Yao,Yang, Hu Cheng,Li, Yu Gu.

[8]Development of a quantitative PCR assay for monitoring Streptococcus agalactiae colonization and tissue tropism in experimentally infected tilapia. Su, Y-L,Li, Y-W,Li, A-X,Su, Y-L,Feng, J.,Bai, J-S.

[9]Proteomic and immunological identification of two new allergens from silkworm (Bombyx mori L.) pupae. Zhao, Xiangjie,Li, Lin,Li, Bing,Zhao, Xiangjie,Kuang, Zheshi,Luo, Guoqing,Zhao, Xiangjie. 2015

[10]Physiological Responses of Watermelon Grafted onto Bottle Gourd to Fusarium oxysporum f. sp niveum Infection. Zhang, M.,Yang, X. P.,Xu, J. H.,Liu, G.,Yao, X. F.,Li, P. F.. 2015

[11]Cloning of the Bacillus thuringiensis serovar sotto chitinase (Schi) gene and characterization of its protein. Fang, Ji-Chao,Cai, Ping-Zhong,Yan, Wen-Zhao,Wu, Jie,Guo, Hui-Fang. 2005

[12]Six chitinases from oriental river prawn Macrobrachium nipponense: cDNA characterization, classification and mRNA expression during post-embryonic development and moulting cycle. Zhang, Shiyong,Fu, Hongtuo,Jiang, Fengwei,Jin, Shubo,Jiang, Sufei,Xiong, Yiwei,Fu, Hongtuo,Sun, Shengming,Qiao, Hui,Zhang, Wenyi,Jin, Shubo,Gong, Yongsheng. 2014

[13]Overexpression of the mulberry latex gene MaMLX-Q1 enhances defense against Plutella xylostella in Arabidopsis thaliana. Liu, Yan,Ji, Dongfeng,Chen, Jine,Lin, Tianbao,Wei, Jia,Zhu, Yan,Lv, Zhiqiang. 2017

[14]Production of N-Acetyl-D-glucosamine from Mycelial Waste by a Combination of Bacterial Chitinases and an Insect N-Acetyl-D-glucosaminidase. Wang, Di,Liu, Tian,Yang, Qing,Zhu, Weixing,Wang, Di,Liu, Tian,Yang, Qing,Yang, Qing.

[15]Characterisation of Isaria fumosorosea isolates and their virulence toward the Diamondback Moth, Plutella xylostella. Xie, Meiqiong,Zhao, Rui,He, Yurong,Wang, Longjiang,Lu, Lihua.

[16]Application of osthol induces a resistance response against powdery mildew in pumpkin leaves. Shi, Zhiqi,Wang, Fei,Zhou, Wei,Zhang, Peng,Fan, Yong Jian. 2007

[17]Microbial Secondary Metabolite, Phlegmacin B-1, as a Novel Inhibitor of Insect Chitinolytic Enzymes. Liu, Tian,Duan, Yanwei,Yang, Qing,Chen, Lei,Liu, Tian,Duan, Yanwei,Yang, Qing,Liu, Tian,Yang, Qing,Lu, Xinhua.

[18]The deduced role of a chitinase containing two nonsynergistic catalytic domains. Zhu, Weixing,Wang, Jing,Zhou, Yong,Duan, Yanwei,Qu, Mingbo,Yang, Qing,Liu, Tian,Zhu, Weixing,Wang, Jing,Zhou, Yong,Duan, Yanwei,Qu, Mingbo,Yang, Qing,Yang, Qing. 2018

[19]Overexpression of a New Chitinase Gene EuCHIT2 Enhances Resistance to Erysiphe cichoracearum DC. in Tobacco Plants. Dong, Xuan,Zhao, Yichen,Ran, Xin,Guo, Linxia,Zhao, De-Gang,Dong, Xuan,Zhao, Yichen,Ran, Xin,Guo, Linxia,Zhao, De-Gang,Dong, Xuan,Ran, Xin,Guo, Linxia,Zhao, De-Gang,Zhao, Yichen,Zhao, De-Gang. 2017

[20]High-yield production of a chitinase from Aeromonas veronii B565 as a potential feed supplement for warm-water aquaculture. Zhang, Yuting,Zhou, Zhigang,Liu, Yuchun,Cao, Yanan,He, Suxu,Huo, Fengmin,Qin, Chubin,Yao, Bin,Ringo, Einar.

作者其他论文 更多>>