Characterization and expression analysis of a chitinase gene (PmChi-5) from black tiger shrimp (Penaeus monodon) under pathogens infection and ambient ammonia-N stress

文献类型： 外文期刊

第一作者： Zhou, Falin

作者： Zhou, Falin;Zhou, Kaimin;Huang, Jianhua;Yang, Qibin;Jiang, Song;Qiu, Lihua;Yang, Lishi;Jiang, Shigui

作者机构：

关键词： Penaeus monodon;Chitinase;Streptococcus agalactiae;Vthrio harveyi;Ammonia nitrogen stress

期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.581； 五年影响因子：4.851 ）

ISSN： 1050-4648

年卷期： 2018 年 72 卷

页码：

收录情况： SCI

摘要： Chitinases are crucial enzymes for crustaceans. Previous researches had already revealed that chitinases play important roles in digestion, molting and defense against viruses. In the present study, a chitinase cDNA was identified from black tiger shrimp (Penaeus monodon) and designated as PmChi-5. The full-length PmChi-5 cDNA was 2860 bp in size, containing an open reading frame (ORF) of 1731 bp that encoded a protein of 576 amino acids with a deduced molecular weight of 64.8 kDa. Expression of the PmChi-5 mRNA was ubiquitously detected in all selected tissues, with the highest level in the gill and hepatopancreas. PmChi-5 was expressed throughout the whole larvae stages, and the highest level at Mysis3 stage, which indicated that PmChi-5 may be involved in larval metamorphosis. After challenged with Streptococcus agalactiae and Vibrio harveyi, the transcripts of PmChi-5 were found to be up-regulated significantly both in hepatopancreas and gill. Besides, the ammonia nitrogen stress treatment was also carried out, PmChi-5 transcripts were significantly changed in hepatopancreas and gill. The results showed that PmChi-5 may be involved in molting, larval metamorphosis, the immune defenses to pathogens infection and ammonia-N stress.

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