Two novel calreticulin-related molecules with microbial binding and phagocytosis enhancing capacity in the half-smooth tongue sole, Cynoglossus semilaevis

文献类型： 外文期刊

第一作者： Wang, Guanghua

作者： Wang, Guanghua;He, Shuwen;Zhang, Min;Jiang, Zengjie;Jiang, Zengjie

作者机构：

关键词： Cynoglossus semilaevis;CsCRTL1;CsCRTL2;Microbial-binding;Phagocytosis enhancing

期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.581； 五年影响因子：4.851 ）

ISSN： 1050-4648

年卷期： 2018 年 72 卷

页码：

收录情况： SCI

摘要： Calreticulin (CRT) is highly conserved chaperone located in the endoplasmic reticulum. It plays important roles in innate immunity. Although various immune-related functions of CRT have been reported in vertebrates and invertebrates, information on the potential functions of teleost CRT is very limited. In the present study, we characterized two calreticulin-related molecules from tongue sole (Cynoglossus semilaevis), calreticulin-likel and calreticulin-like2 (CsCRTL1 and CsCRTL2). CsCRTLland CsCRTL2 contain signature CRT motifs that are highly conserved in different species. CsCRTLland CsCRTL2 were expressed in liver, head kidney, brain, spleen, heart, muscle, skin, intestine and gills. The expression levels of CsCRTLland CsCRTL2 were highest in liver and spleen, respectively. After stimulation by Vibrio anguillarum and Streptococcus agalactiae, CsCRTL1 and CsCRTL2 were significantly up-regulated. The expression patterns depended on the tissue type, pathogen type, and infection time. The recombinant proteins rCsCRTLland rCsCRTL2 bound to different pathogen-associated molecular patterns (PAMPs) including LPS and PGN, and to different bacteria, such as Gram-negative bacteria V. anguillarum and Gram-positive bacteria Staphylococcus aureus. Moreover, rCsCRTLland rCsCRTL2 significantly enhanced the killing of V. anguillarum by tongue sole macrophages. Our results indicate that CsCRTLland CsCRTL2 play important roles in antibacterial immunity of tongue sole.

分类号：

  • 相关文献

[1]Induction of Mitogynogenetic Diploids and Identification of WW Super-female Using Sex-Specific SSR Markers in Half-Smooth Tongue Sole (Cynoglossus semilaevis). Chen, Song-Lin,Ji, Xiang-Shan,Shao, Chang-Wei,Li, Wen-Long,Yang, Jing-Feng,Liang, Zuo,Liao, Xiao-Lin,Xu, Gen-Bo,Xu, Ying,Song, Wen-Tao,Ji, Xiang-Shan. 2012

[2]Molecular characterization and expression profiles of LPXRFa at the brain pituitary-gonad axis of half-smooth tongue sole (Cynoglossus semilaevis) during ovarian maturation. Wang, Bin,Liu, Quan,Liu, Xuezhou,Xu, Yongjiang,Shi, Bao,Wang, Bin,Liu, Xuezhou,Xu, Yongjiang,Shi, Bao,Liu, Quan. 2018

[3]Effects of different dietary DHA: EPA ratios on gonadal steroidogenesis in the marine teleost, tongue sole (Cynoglossus semilaevis). Xu, Houguo,Cao, Lin,Wei, Yuliang,Zhang, Yuanqin,Liang, Mengqing,Liang, Mengqing. 2017

[4]Evaluation of reference genes for quantitative real-time PCR analysis of gene expression during early development processes of the tongue sole (Cynoglossus semilaevis). Ma Qian,Zhuang Zhimeng,Feng Wenrong,Liu Shufang,Tang Qisheng. 2015

[5]Growth differences and differential expression analysis of pituitary adenylate cyclase activating polypeptide (PACAP) and growth hormone-releasing hormone (GHRH) between the sexes in half-smooth tongue sole Cynoglossus semilaevis. Ji, Xiang-Shan,Chen, Song-Lin,Xu, Tian-Jun,Yang, Jing-Feng,Tian, Yong-Sheng,Ji, Xiang-Shan,Jiang, Yun-Liang. 2011

[6]Development and characterization of 60 novel EST-SSR markers in half-smooth tongue sole Cynoglossus semilaevis. Sha, Z-X.,Luo, X-H.,Liao, X-L.,Wang, Q-L.,Chen, S-L.,Wang, S-L.,Wang, S-L.. 2011

[7]Molecular cloning, characterization and expression analysis of Sox9a and Foxl2 genes in half-smooth tongue sole (Cynoglossussemilaevis). Dong Xiaoli,Chen Songlin,Shao Changwei,Dong Xiaoli,Ji Xiangshan. 2011

[8]Molecular characterization of Pod1 during sex development in Chinese tongue sole (Cynoglossus semilaevis). Wang, Linna,Zhu, Ying,Xu, Wenteng,Shao, Changwei,Dong, Zhongdian,Li, Hailong,Cui, Zhongkai,Meng, Liang,Guo, Hua,Tian, Yongsheng,Chen, Songlin,Wang, Linna,Xu, Wenteng,Shao, Changwei,Tian, Yongsheng,Chen, Songlin. 2017

[9]Sensory bases and the mode of feeding behavior of tongue sole Cynoglossus semilaevis. Wang Xinan,Ma Aijun. 2009

[10]Effects of BPA and E2 on expression profiles of genes related to hypothalamic-pituitary-gonadal axis of half-smooth tongue sole Cynoglossus semilaevis. Li Fengling,Li Zhaoxin,Wang Qingyin,Zhai Yuxiu. 2013

[11]Artificial gynogenesis in Cynoglossus semilaevis with homologous sperm and its verification using microsatellite markers. Chen, Song-Lin,Ji, Xiang-Shan,Jiang, Yun-Liang. 2010

[12]Polymorphic dinucleotide microsatellites in tongue sole (Cynoglossus semilaevis). Liao, X.,Shao, C. W.,Tian, Y. S.,Chen, S. L.. 2007

[13]Genetic variability of half-smooth tongue sole Cynoglossus semilaevis populations using microsatellite markers. Liu Shuaishuai,Ye Naihao,Liu Yunguo,Liu Yunguo,Liu Shuaishuai,Guo Yinghui,Li Junfeng. 2011

[14]Histopathological Liver and Testis Alterations in Male Half Smooth Tongue Sole (Cynoglossus semilaevis) Exposed to Endocrine Disruptors. Li, Fengling,Yao, Lin,Sun, Weihong,Jiang, Yanhua,Li, Zhaoxin,Zhai, Yuxiu. 2017

[15]A first generation BAC-based physical map of the half-smooth tongue sole (Cynoglossus semilaevis) genome. Zhang, Junjie,Shao, Changwei,Zhang, Liyan,Liu, Kun,Gao, Fengtao,Dong, Zhongdian,Chen, Songlin,Zhang, Junjie,Zhang, Junjie,Xu, Peng. 2014

[16]Application of AFLP markers for population genetic study on half-smooth tongue sole Cynoglossus semilaevis. Liu Yunguo,Liu Yunguo,Li Junfeng,Ye Naihao. 2011

[17]Growth and survival of tongue sole (Cynoglossus semilaevis Gunther, 1873) larvae fed a compound diet with different protein sources. Chang, Q,Liang, MQ,Wang, JL,Chen, SQ,Zhang, XM,Liu, XD. 2006

[18]Antarctic krill (Euphausia superba) meal in the diets improved the reproductive performance of tongue sole (Cynoglossus semilaevis) broodstock. Xu, H. G.,Zhao, M.,Zheng, K. K.,Wei, Y. L.,Liang, M. Q.,Xu, H. G.,Zheng, K. K.,Liang, M. Q.,Zhao, M.,Yan, L.. 2017

[19]Studies on the ultrastructure of spermiogenesis and spermatozoon of tongue fish, Cynoglossus semilaevis gunther. Wu, Yingying,Liu, Xuezhou,Wang, Qingyin,Xu, Yongjiang,Wu, Yingying,Bao, Zhenmin. 2008

[20]Cloning, expression profiling and promoter functional analysis of bone morphogenetic protein 2 in the tongue sole (Cynoglossus semilaevis). Ma, Qian,Fan, Yanjun,Zhuang, Zhimeng,Liu, Shufang,Ma, Qian,Liu, Shufang,Zhuang, Zhimeng. 2018

作者其他论文 更多>>

微信小程序