Effects of dietary poly-beta-hydroxybutyrate (PHB) on microbiota composition and the mTOR signaling pathway in the intestines of litopenaeus vannamei

文献类型： 外文期刊

第一作者： Duan, Yafei

作者： Duan, Yafei;Zhang, Yue;Dong, Hongbiao;Wang, Yun;Zhang, Jiasong

作者机构：

关键词： Litopenaeus vannamei;poly-beta-hydroxybutyrate (PHB);prebiotic;intestine microbiota;mTOR signaling

期刊名称：JOURNAL OF MICROBIOLOGY （ 影响因子：3.422； 五年影响因子：3.28 ）

ISSN： 1225-8873

年卷期： 2017 年 55 卷 12 期

页码：

收录情况： SCI

摘要： Poly-beta-hydroxybutyrate (PHB) is a natural polymer of the short chain fatty acid beta-hydroxybutyrate, which acts as a microbial control agent. The mammalian target of the rapamycin (mTOR) signaling pathway plays a crucial role in intestine inflammation and epithelial morphogenesis. In this study, we examined the composition of intestine microbiota, and mTOR signaling-related gene expression in Pacific white shrimp Litopenaeus vannamei fed diets containing different levels of PHB: 0% (Control), 1% (PHB1), 3% (PHB3), and 5% (PHB5) (w/w) for 35 days. High-throughput sequencing analysis revealed that dietary PHB altered the composition and diversity of intestine microbiota, and that the microbiota diversity decreased with the increasing doses of PHB. Specifically, dietary PHB increased the relative abundance of Proteobacteria and Tenericutes in the PHB1 and PHB5 groups, respectively, and increased that of Gammaproteobacteria in the three PHB groups. Alternatively, PHB decreased Alphaproteobacteria in the PHB3 and PHB5 groups. At the genus level, dietary PHB increased the abundance of beneficial bacteria, such as Bacillus, Lactobacillus, Lactococcus, Clostridium, and Bdellovibrio. The relative mRNA expression levels of the mTOR signaling-related genes TOR, 4E-BP, eIF4E1 alpha, and eIF4E2 all increased in the three PHB treatment groups. These results revealed that dietary PHB supplementation had a beneficial effect on intestine health of L. vannamei by modulating the composition of intestine microbiota and activating mTOR signaling.

分类号：

  • 相关文献

[1]Alpha-ketoglutarate inhibits glutamine degradation and enhances protein synthesis in intestinal porcine epithelial cells. Yao, Kang,Yin, Yulong,Wu, Guoyao,Yao, Kang,Yin, Yulong,Wu, Guoyao,Yao, Kang,Li, Xilong,Xi, Pengbin,Wang, Junjun,Lei, Jian,Wu, Guoyao,Xi, Pengbin,Wang, Junjun,Wu, Guoyao,Hou, Yongqing.

[2]Utility of Thermostable Xylanases of Mycothermus thermophilus in Generating Prebiotic Xylooligosaccharides. Ma, Rui,Bai, Yingguo,Huang, Huoqing,Luo, Huiying,Yao, Bin,Ma, Rui,Fan, Yunliu,Ma, Rui,Chen, Sanfeng,Cai, Lei.

[3]Effects of soybean oligosaccharides on intestinal microbial communities and immune modulation in mice. Ma, Yan,Meng, Xianjun,Wu, Xingzhuang,Giovanni, Vigna. 2017

[4]Effects of dietary chitosan oligosaccharides and Bacillus coagulans on the growth, innate immunity and resistance of koi (Cyprinus carpio koi). Lin, Shimei,Mao, Shuhong,Guan, Yong,Luo, Li,Pan, Yu,Lin, Shimei,Luo, Li,Luo, Lin. 2012

[5]Antioxidant activity of prebiotic ginseng polysaccharides combined with potential probiotic Lactobacillus plantarum C88. He, Zhongmei,Wang, Xiaohui,Wang, Xiaohui,Zhao, Yujuan,Zhang, Jian,Niu, Chunhua,Li, Shengyu,Li, Guofeng,Ying, Dashi,Zhang, Li,Zhang, Xue.

[6]Prebiotic Potential of Xylooligosaccharides Derived from Corn Cobs and Their In Vitro Antioxidant Activity When Combined with Lactobacillus. Yu, Xiuhua,Yin, Jianyuan,Zhao, Chunfang,Yu, Xiuhua,Li, Lin,Luan, Chang,Zhang, Jian,Li, Shengyu.

[7]Synbiotic dietary supplement affects growth, immune responses and intestinal microbiota of Apostichopus japonicus. Wang, Xitao,Wang, Lili,Li, Xiaoyu,Qu, Kunli,Xu, Yongping,Sun, Yongxin,Li, Xiaoyu,Xu, Yongping.

[8]Identification of SNP markers associated with tolerance to ammonia toxicity by selective genotyping from de novo assembled transcriptome in Litopenaeus vannamei. Lu, Xia,Kong, Jie,Meng, Xianhong,Cao, Baoxiang,Luo, Kun,Dai, Ping,Luan, Sheng,Lu, Xia,Kong, Jie,Meng, Xianhong,Cao, Baoxiang,Luo, Kun,Dai, Ping,Luan, Sheng. 2018

[9]Effects of different enzymatic hydrolysis methods on the bioactivity of peptidoglycan in Litopenaeus vannamei. Song Xiaoling,Zhang Yue,Wei Song,Huang Jie,Zhang Yue. 2013

[10]Excess dietary cholesterol may have an adverse effect on growth performance of early post-larval Litopenaeus vannamei. Niu, Jin,Lin, Hei-Zhao,Chen, Peng-Fei,Tian, Li-Xia,Liu, Yong-Jian,Yang, Hui-Jun,Liang, Gui-Ying. 2012

[11]Dietary Methionine Requirements of Pacific White Shrimp Litopenaeus vannamei, of Three Different Sizes. Lin, Heizhao,Chen, Yifang,Niu, Jin,Zhou, Chuanpeng,Huang, Zhong,Du, Qiang. 2015

[12]White spot syndrome virus VP12 interacts with adenine nucleotide translocase of Litopenaeus vannamei. Ma, Fang-fang,Chou, Zhi-guang,Liu, Qing-hui,Guan, Guangkuo,Li, Chen,Huang, Jie,Ma, Fang-fang,Guan, Guangkuo. 2014

[13]Effect of dietary traditional Chinese medicines on apparent digestibility coefficients of nutrients for white shrimp Litopenaeus vannamei, Boone. Lin, HZ,Li, ZJ,Chen, YQ,Zheng, WH,Yang, K. 2006

[14]Effects of bioflocs on water quality, and survival, growth and digestive enzyme activities of Litopenaeus vannamei (Boone) in zero-water exchange culture tanks. Xu, Wu Jie,Pan, Lu Qing,Sun, Xiao Hua,Huang, Jie. 2013

[15]Effects of seawater potassium concentration on the dietary potassium requirement of Litopenaeus vannamei. Zhu, Chang-Bo,Dong, Shuang-Lin,Wang, Fang,Zhang, Han-Hua. 2006

[16]Effect of periodic light color change on the molting frequency and growth of Litopenaeus vannamei. Guo, Biao,Wang, Fang,Dong, Shuanglin,Guo, Biao,Mu, Yingchun. 2012

[17]Physiological responses to swimming fatigue of juvenile white-leg shrimp Litopenaeus vannamei exposed to different current velocities, temperatures and salinities. Zhang, P. D.,Zhang, X. M.,Li, J.. 2011

[18]Effects of dietary chitosan on growth, survival and stress tolerance of postlarval shrimp, Litopenaeus vannamei. Niu, J.,Liu, Y-J,Yang, H-J,Liang, G-Y,Tian, L-X,Niu, J.,Lin, H-Z,Mai, K-S. 2011

[19]Isolation and characterization of microsatellite markers from Pacific white shrimp (Litopenaeus vannamei). Jia Zhi-Ying,Sun Xiao-Wen,Liang Li-Qun,Li Da-Yu,Lei Qing-Quan. 2006

[20]Effect of bioactive peptides (BPs) on the development of Pacific white shrimp (Litopenaeus vannamei Boone, 1931). Wang Guangjun,Yu Ermeng,Li Zhifei,Yu Deguang,Wang Haiying,Gong Wangbao. 2016

作者其他论文 更多>>

微信小程序