Development and characterization of SNP derived from spinyhead croaker (Collichthys lucidus) by RNA-seq

文献类型： 外文期刊

第一作者： Chen, Wei

作者： Chen, Wei;Song, Wei;Chen, Fenfang;Zhao, Ming;Ma, Chunyan;Zhang, Fengying;Wang, Wei;Ma, Lingbo;Chen, Fenfang

作者机构：

关键词： Collichthys lucidus;SNP;HiSeq 2000;Sequenom MassARRAY

期刊名称：CONSERVATION GENETICS RESOURCES （ 影响因子：0.973； 五年影响因子：0.946 ）

ISSN： 1877-7252

年卷期： 2017 年 9 卷 4 期

页码：

收录情况： SCI

摘要： A novel set of SNP markers were detected from RNA-seq data of spinyhead croaker (Collichthys lucidus). Totally 49,617,970 clean reads and 78,671 putative SNPs were obtained. Among them, 40 SNPs were randomly selected to perform validation in 30 wild individuals of C. lucidus captured from 3 geographic stocks in the coast of China. Eventually, 29 SNP loci were successfully validated and showed bi-allelic polymorphism in genotyping by Sequenom MassARRAY. These novel SNPs provided useful genomic resources for C. lucidus, especially in population genetics, molecular assisted selection and conservation studies.

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