Identification, cloning and expression analysis of an alpha-CGTase produced by stain Y112

文献类型： 外文期刊

第一作者： Hao, Jian-Hua

作者： Hao, Jian-Hua;Sun, Jing-Jing;Liu, Jun-Zhong;Wang, Wei;Sun, Mi;Hao, Jian-Hua;Sun, Jing-Jing;Liu, Jun-Zhong;Wang, Wei;Sun, Mi;Huang, Li-Ping;Chen, Xiao-tong

作者机构：

关键词： alpha-Cyclodextrin glycosyltransferase;Cloning;Expression

期刊名称：PROTEIN EXPRESSION AND PURIFICATION （ 影响因子：1.65； 五年影响因子：1.548 ）

ISSN： 1046-5928

年卷期： 2017 年 140 卷

页码：

收录情况： SCI

摘要： Cyclodextrin glycosyltransferase (CGTase) is an enzyme able to convert starch and other substrates into cyclodextrins (CDs). A marine strain Y112 producing alpha-CGTase was identified as Bacillus agaradhaerens Y112 by physiological and biochemical characterization, and 16S rDNA analysis. The gene coding for alpha-CGTase was cloned, sequenced and expressed in Escherichia coli BL21 (DE3) cells. Recombinant alpha-CGTase was purified in one-step chromatographic separation and its purity evaluated by SDS-PAGE, showing the presence of one band with a molecular mass of about 92 kDa. Additionally, enzymatic capability was analyzed by measuring the starch conversion, and resulted in about 45% of CDs obtained after 6 h of cyclodextrin reaction. Of these CDs, mainly alpha-CD was produced (70% of the total CDs yield), suggesting the potential of this CGTase for industrial applications. (C) 2017 Elsevier Inc. All rights reserved.

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