Identification, cloning and expression analysis of an alpha-CGTase produced by stain Y112
文献类型: 外文期刊
第一作者: Hao, Jian-Hua
作者: Hao, Jian-Hua;Sun, Jing-Jing;Liu, Jun-Zhong;Wang, Wei;Sun, Mi;Hao, Jian-Hua;Sun, Jing-Jing;Liu, Jun-Zhong;Wang, Wei;Sun, Mi;Huang, Li-Ping;Chen, Xiao-tong
作者机构:
关键词: alpha-Cyclodextrin glycosyltransferase;Cloning;Expression
期刊名称:PROTEIN EXPRESSION AND PURIFICATION ( 影响因子:1.65; 五年影响因子:1.548 )
ISSN: 1046-5928
年卷期: 2017 年 140 卷
页码:
收录情况: SCI
摘要: Cyclodextrin glycosyltransferase (CGTase) is an enzyme able to convert starch and other substrates into cyclodextrins (CDs). A marine strain Y112 producing alpha-CGTase was identified as Bacillus agaradhaerens Y112 by physiological and biochemical characterization, and 16S rDNA analysis. The gene coding for alpha-CGTase was cloned, sequenced and expressed in Escherichia coli BL21 (DE3) cells. Recombinant alpha-CGTase was purified in one-step chromatographic separation and its purity evaluated by SDS-PAGE, showing the presence of one band with a molecular mass of about 92 kDa. Additionally, enzymatic capability was analyzed by measuring the starch conversion, and resulted in about 45% of CDs obtained after 6 h of cyclodextrin reaction. Of these CDs, mainly alpha-CD was produced (70% of the total CDs yield), suggesting the potential of this CGTase for industrial applications. (C) 2017 Elsevier Inc. All rights reserved.
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