Probing the mechanical stability of bridged DNA-H-NS protein complexes by single-molecule AFM pulling

文献类型： 外文期刊

第一作者： Liang, Yan

作者： Liang, Yan;Liang, Yan;Liang, Yan;Dame, Remus T.;Roos, Wouter H.;Wuite, Gijs J. L.;Liang, Yan;Dame, Remus T.;Roos, Wouter H.;Wuite, Gijs J. L.;van der Valk, Ramon A.;Dame, Remus T.;Roos, Wouter H.

作者机构：

期刊名称：SCIENTIFIC REPORTS （ 影响因子：4.379； 五年影响因子：5.133 ）

ISSN： 2045-2322

年卷期： 2017 年 7 卷

页码：

收录情况： SCI

摘要： Atomic force microscopy (AFM) has proven to be a powerful tool for the study of DNA-protein interactions due to its ability to image single molecules at the nanoscale. However, the use of AFM in force spectroscopy to study DNA-protein interactions has been limited. Here we developed a high throughput, AFM based, pulling assay to measure the strength and kinetics of protein bridging of DNA molecules. As a model system, we investigated the interactions between DNA and the Histone-like Nucleoid-Structuring protein (H-NS). We confirmed that H-NS both changes DNA rigidity and forms bridges between DNA molecules. This straightforward methodology provides a high-throughput approach with single-molecule resolution which is widely applicable to study cross-substrate interactions such as DNA-bridging proteins.

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