Transparent Tiger barb Puntius tetrazona, a fish model for in vivo analysis of nocardial infection

文献类型： 外文期刊

第一作者： Wang, F.

作者： Wang, F.;Liv, C.;Chang, O. Q.;Feng, Y. Y.;Jiang, L.;Li, K. B.;Wang, X. G.

作者机构：

关键词： Puntius tetrazona;transparent;leukocyte;in vivo;Nocardia seriolae;granuloma

期刊名称：VETERINARY MICROBIOLOGY （ 影响因子：3.293； 五年影响因子：3.599 ）

ISSN： 0378-1135

年卷期： 2017 年 211 卷

页码：

收录情况： SCI

摘要： Nocardiosis afflicts multiple species of cultured fish, resulting in substantial economic losses to the aquaculture industry, however, lack of detailed knowledge on disease pathogenesis has hampered the development of effective prevention and control strategies. In this study, we injected a green fluorescent protein (GFP)-labeled Nocardia seriolae strain into a transparent mutant strain of Tiger barb (Puntius tetrazona) to monitor tissue pathogen accumulation and tissue damage in vivo, and to clarify the relationship between pathogenic processes and overt symptoms. GFP-labeled bacteria were phagocytized by leukocytes and could proliferate within these cells, which in turn led to leukocyte aggregation, leukocyte death, and granuloma formation. In addition, intracellular bacteria could permanently colonize various tissues via leukocyte circulation, causing multi-organ infection as revealed by changes of tissue transparency. Histology revealed granulomatous lesions in organs such as muscle, kidney, and spleen that was corresponded to the tissue opacities in vivo. Confocal microscopy confirmed massive accumulations of GFP-labeled bacteria within these granulomas, which often contained a necrotic core. Tiger barb transparency allows for real-time observation of in vivo pathological changes within the same animal, and the pathogenic process can be evaluated based on the shape and size of body opacities. Thus, transparent Tiger barb is a promising model to study the pathogenesis of nocardiosis.

分类号：

  • 相关文献

[1]Edwardsiellosis in farmed turbot, Scophthalmus maximus (L.), associated with an unusual variant of Edwardsiella tarda: a clinical, aetiological and histopathological study. Qin, L.,Xu, J.,Wang, Y. G.. 2014

[2]Nocardia seriolae infection in cultured jade perch, Scortum barcoo. Wang, Fang,Liu, Chun,Chang, Ouqin,Feng, Yongyong,Jiang, Lang,Li, Kaibin,Wang, Xuguang. 2017

[3]Atypical Classical Swine Fever Infection Changes Interleukin Gene Expression in Pigs. Sun, Y. K.,Sun, Y. K.,Zhang, X. M.,Du, M.,Li, Y. X.,Pan, H. B.,Yan, Y. L.,Yang, Y. A..

[4]Identification of a transparent mutant tiger barb Puntius tetrazona and its use for in vivo observation of a Pleistophora sp (Microsporidia) infection. Li, K. B.,Chang, O. Q.,Wang, F.,Liu, C.,Wang, Q.,Liang, F. L.,Ma, B. Y.,Wu, S. Q..

[5]Carbon adaptation influence the antagonistic ability of Pseudomonas aeruginosa against Fusarium oxysporum f. sp melonis. Wang, Yanli,Sun, Guochang,Li, Bin,Tang, Qiaomei,Chen, Xiaoling,Xie, Guanlin,Li, Hongye,Yu, Rongrong,Wu, Zhiyi. 2011

[6]In vitro and in vivo antioxidative and radioprotective capacities of polysaccharide isolated from Mesona Blumes gum. Feng, Tao,Sang, Min,Zhuang, Haining,Xu, Zhimin,Zhuang, Haining,Xu, Zhimin. 2017

[7]Gene Expression of Type VI Secretion System Associated with Environmental Survival in Acidovorax avenae subsp avenae by Principle Component Analysis. Cui, Zhouqi,Li, Bin,Kakar, Kaleem Ullah,Ojaghian, Mohammad Reza,Xie, Guanlin,Jin, Guoqiang,Wang, Yangli,Sun, Guochang. 2015

[8]Erythromycin Resistance of Streptococcus suis Isolates In Vivo. Jiang Cheng-gang,Cai Xue-hui,Qiu Hua-ji,Li Yan-hua,Tong Heng-min,Liu Di-qiu,Lei Lian-cheng,Sun Chang-jiang. 2009

[9]Innate Immune Responses in ALV-J Infected Chicks and Chickens with Hemangioma In Vivo. Feng, Min,Xie, Tingting,Li, Zhenhui,Zhang, Xiquan,Feng, Min,Xie, Tingting,Li, Zhenhui,Zhang, Xiquan,Feng, Min,Xie, Tingting,Li, Zhenhui,Zhang, Xiquan,Dai, Manman,Shi, Meiqing. 2016

[10]In-Field, In Situ, and In Vivo 3-Dimensional Elemental Mapping for Plant Tissue and Soil Analysis Using Laser-Induced Breakdown Spectroscopy. Zhao, Chunjiang,Dong, Daming,Du, Xiaofan,Zheng, Wengang. 2016

[11]Comprehensive Analysis of Tiamulin Metabolites in Various Species of Farm Animals Using Ultra-High-Performance Liquid Chromatography Coupled to Quadrupole/Time-of-Flight. Sun, Feifei,Zhang, Huiyan,Wang, Zhanhui,Shen, Jianzhong,Zhang, Suxia,Cao, Xingyuan,Sun, Feifei,Zhang, Huiyan,Wang, Zhanhui,Shen, Jianzhong,Zhang, Suxia,Cao, Xingyuan,Yang, Shupeng,Zhou, Jinhui,Li, Yi,Zhang, Jinzhen,Jin, Yue,Yang, Shupeng,Zhou, Jinhui,Li, Yi,Zhang, Jinzhen,Jin, Yue,Li, Yanshen.

[12]ZIP restores estrogen receptor expression and response to Tamoxifen in estrogen receptor negative tumors. Zhu, Ning,Miao, Ruidong,Du, Yuping,Yang, Jinbo,Zhang, Jing,Qin, Xiaodong.

[13]Sulforaphane reduces vascular inflammation in mice and prevents TNF-alpha-induced monocyte adhesion to primary endothelial cells through interfering with the NF-kappa B pathway. Nallasamy, Palanisamy,Brooke, Elizabeth A. S.,Shah, Halley,Jia, Zhenquan,Si, Hongwei,Babu, Pon Velayutham Anandh,Pan, Dengke,Fu, Yu,Zhen, Wei,Liu, Dongmin,Fu, Yu,Zhen, Wei,Zhu, Hong,Li, Yunbo.

[14]Identification of in vivo protein phosphorylation sites in human pathogen Schistosoma japonicum by a phosphoproteomic approach. Luo, Rong,Zhou, Chunjing,Lin, Jiaojiao,Yang, Dehao,Shi, Yaojun,Cheng, Guofeng.

[15]Ovulation, fertilization and preimplantation embryonic development in raccoon dogs (Nyctereutes procyonoides). Xu, Baozeng,Xu, Baozeng,Feng, Huai L..

[16]Microsensor in vivo monitoring of oxidative burst in oilseed rape (Brassica napus L.) leaves infected by Sclerotinia sclerotiorum. Xu, Qiao,Liu, Sheng-Yi,Guo, Xue-Lan,Dong, Xu-Yan,Li, Pei-Wu,Chen, Hong,Xu, Qiao,Zou, Qiu-Ju,Zhao, Yuan-Di,Xu, Qiao,Zou, Qiu-Ju,Zhao, Yuan-Di,Song, Dan-Yang,Chen, Hong.

[17]Porcine Mx1 fused to HIV Tat protein transduction domain (PTD) inhibits classical swine fever virus infection in vitro and in vivo. Jing, Jiao,Shi, Baojun,Xu, Qianqian,Zhou, Bin,Chen, Puyan,Li, Wenliang,Liu, Ke,Ma, Zhiyong. 2015

[18]Angiotensin I Converting Enzyme (ACE) inhibitory activity and antihypertensive effects of grass carp peptides. Wang Shen,Lin Li-mei,Wu Yong-ning,Fang Min,Yu Ya-qin,Zhou Jia,Gong Zhi-yong,Wu Yong-ning. 2014

[19]Membrane protein profiling of Acidovorax avenae subsp avenae under various growth conditions. Li, Bin,Wang, Li,Ge, Mengyu,Wang, Yanli,Sun, Guochang,Ibrahim, Muhammad,Mannan, Shazia,Asif, Muhammad.

[20]Long terminal repeat sequences from virulent and attenuated equine infectious anemia virus demonstrate distinct promoter activities. Zhou, Tao,Yuan, Xiu-Fang,Hou, Shao-Hua,Tu, Ya-Bin,Peng, Jin-Mei,Wen, Han-Xin,Qiu, Hua-H,Wu, Dong-Lai,Chen, Huan-Chun,Wang, Xiao-Jun,Tong, Guang-Zhi. 2007

作者其他论文 更多>>

微信小程序