Molecular characterization of kiss2 and differential regulation of reproduction-related genes by sex steroids in the hypothalamus of half-smooth tongue sole (Cynoglossus semilaevis)

文献类型: 外文期刊

第一作者: Wang, Bin

作者: Wang, Bin;Liu, Quan;Liu, Xuezhou;Xu, Yongjiang;Song, Xuesong;Shi, Bao;Wang, Bin;Liu, Xuezhou;Xu, Yongjiang;Shi, Bao;Liu, Quan;Song, Xuesong

作者机构:

关键词: Kisspeptin;Sex steroids;Gene expression;Cynoglossus semilaevis

期刊名称:COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY ( 影响因子:2.32; 五年影响因子:2.465 )

ISSN: 1095-6433

年卷期: 2017 年 213 卷

页码:

收录情况: SCI

摘要: Kisspeptin (Kiss) plays a critical role in mediating gonadal steroid feedback to the gonadotropin-releasing hormone (GnRH) neurons in mammals. However, little information regarding the regulation of kisspeptin gene by sex steroids is available in teleosts. In this study, we examined the direct actions of estradiol (E2) and testosterone (T) on hypothalamic expression of kisspeptin and other key factors involved in reproductive function of half-smooth tongue sole. As a first step, a partial-length cDNA of kiss2 was identified from the brain of tongue sole and kiss2 transcript levels were shown to be widely expressed in various tissues, notably in the ovary. Then, the actions of sex steroids on kiss2 and other reproduction-related genes were evaluated using a primary hypothalamus culture system. Our results showed that neither kiss2 nor its receptor kiss2r mRNA levels were significantly altered by sex steroids. Moreover, sex steroids did not modify hypothalamic expression of gonadotropin-inhibitory hormone (gnih) and its receptor gnihr mRNAs, either. However, E2 markedly stimulated both gnrh2 and gnrh3 mRNAs levels. Overall, this study provides insights into the role of sex steroids in the reproductive function of Pleuronectiform teleosts.

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