Optimization of protectant, salinity and freezing condition for freeze-drying preservation of Edwardsiella tarda

文献类型： 外文期刊

第一作者： Yu, Yongxiang

作者： Yu, Yongxiang;Xue, Liangyi;Yu, Yongxiang;Zhang, Zheng;Wang, Yingeng;Liao, Meijie;Li, Bin

作者机构：

关键词： freeze-drying;mariculture;pathogen;Edwardsiella tarda;protectant;orthogonal test;interaction effect

期刊名称：JOURNAL OF OCEAN UNIVERSITY OF CHINA （ 影响因子：0.913； 五年影响因子：1.012 ）

ISSN： 1672-5182

年卷期： 2017 年 16 卷 5 期

页码：

收录情况： SCI

摘要： Novel preservation condition without ultra-low temperature is needed for the study of pathogen in marine fishes. Freeze-drying is such a method usually used for preservation of terrigenous bacteria. However, studies using freeze-drying method to preserving marine microorganisms remain very limited. In this study, we optimized the composition of protectants during the freeze-drying of Edwardsiella tarda, a fish pathogen that causes systemic infection in marine fishes. We found that the optimal composition of protectant mixture contained trehalose (8.0%), skim milk (12.0%), sodium citrate (2.0%), serum (12.0%) and PVP (2.0%). Orthogonal and interaction analyses demonstrated the interaction between serum and skim milk or sodium citrate. The highest survival rate of E. tarda was observed when the concentration of NaCl was 10.0, 30.0 and between 5.0 and 10.0 g L-1 for preparing TSB medium, E. tarda suspension and protectant mixture, respectively. When E. tarda was frozen at -80A degrees C or -40A degrees C for 6 h, its survival rate was higher than that under other tested conditions. Under the optimized conditions, when the protectant mixture was used during freeze-drying process, the survival rate (79.63%-82.30%) of E. tarda was significantly higher than that obtained using single protectant. Scanning electron microscopy (SEM) image indicated that E. tarda was embedded in thick matrix with detectable aggregation. In sum, the protectant mixture may be used as a novel cryoprotective additive for E. tarda.

分类号：

  • 相关文献

[1]Effects of Different Drying Methods and Extraction Condition on Antioxidant Properties of Shiitake (Lentinus edodes). Zhang, Zuofa,Lv, Guoying,Pan, Huijuan,Wu, Yongzhi,Fan, Leifa.

[2]Effects of salinity, light and temperature on growth rates of two species of Gracilaria (Rhodophyta). Xu Yongjian,Fang Jianguang,Xu Yongjian,Wei Wei. 2009

[3]Negative interactive effects between biochar and phosphorus fertilization on phosphorus availability and plant yield in saline sodic soil. Xu, Gang,Zhang, You,Sun, Junna,Shao, Hongbo,Shao, Hongbo,Sun, Junna,Zhang, You.

[4]OPTIMIZATION OF ILLUMINATION ENVIRONMENTAL FACTORS BASED ON ORTHOGONAL TEST. Liu, Libo,Liu, Libo,Zhou, Guomin. 2010

[5]Research on the Production of Biological Diesel Oil Using Gold-thread Tung Oil. Zheng, W.,Tian, G. Z.,Liu, J. L.. 2015

[6]Study on Compost Conditions and Nitrogen Changes of Poultry Dung with Banana Tree. Zhang, Yubai,Tang, Xuexi,Li, Qinfen. 2013

[7]Structure optimization of CycloBio fluidized sand biofilters based on numerical simulation. Liu, Yao,Huang, Zhi-Tao,Song, Xie-Fa,Peng, Lei,Liu, Yao,Lei, Ji-Lin,Liu, Bao-Liang.

[8]Metabolic and Phylogenetic Profile of Bacterial Community in Guishan Coastal Water (Pearl River Estuary), South China Sea. Hu Xiaojuan,Liu Qing,Yang Yufeng,Hu Xiaojuan,Li Zhuojia,Cao Yucheng,He Zhili,He Zhili,Gong Yingxue,Yang Yufeng. 2014

[9]Risk assessment and seasonal variation of heavy metals in settling particulate matter (SPM) from a typical southern Chinese mariculture base. Gu, Yang-Guang,Jun Ouyang,An, Hong,Jiang, Shi-Jun,Tang, Hong-Qu,Gu, Yang-Guang,Jiang, Shi-Jun,Tang, Hong-Qu,Tang, Hong-Qu. 2017

[10]The budget of dissolved inorganic carbon in the shellfish and seaweed integrated mariculture area of Sanggou Bay, Shandong, China. Jiang, Zengjie,Li, Jiaqi,Jiang, Xu,Wang, Wei,Fang, Jianguang,Qiao, Xudong,Huang, Daji,Wang, Guanghua,Bian, Dapeng,Liu, Yan.

[11]Marine fungal communities in water and surface sediment of a sea cucumber farming system: habitat-differentiated distribution and nutrients driving succession. Guo, Xiaohong,Zhang, Qianqian,Zhang, Xiaoli,Gong, Jun,Guo, Xiaohong,Zhang, Jiasong.

[12]Impacts of mariculture practices on the temporal distribution of macrobenthos in Sandu Bay, South China. Zhou Jin. 2012

[13]Effects of freshwater input and mariculture (bivalves and macroalgae) on spatial distribution of nanoflagellates in Sungo Bay, China. Lu, Jiachang,Wu, Linnan,Huang, Lingfeng,Luo, Yuanrong,Xiao, Tian,Jiang, Zengjie.

[14]Levels, composition profiles and sources of polycyclic aromatic hydrocarbons in surface sediments from Nan'ao Island, a representative mariculture base in South China. Gu, Yang-Guang,Lin, Qin,Lu, Teng-Teng,Ke, Chang-Liang,Sun, Run-Xia,Du, Fei-Yan,Gu, Yang-Guang,Lin, Qin,Lu, Teng-Teng,Ke, Chang-Liang,Sun, Run-Xia,Du, Fei-Yan,Gu, Yang-Guang,Lin, Qin,Lu, Teng-Teng,Ke, Chang-Liang,Sun, Run-Xia,Du, Fei-Yan,Lu, Teng-Teng.

[15]An improved method for detection of Edwardsiella tarda by loop-mediated isothermal amplification by targeting the EsrB gene. Xie Guosi,Zhang Qingli,Han Nana,Shi Chengyin,Wang Xiuhua,Liu Qinghui,Huang Jie,Xie Guosi,Huang Jie. 2012

[16]Development and validation of a TaqMan (TM) fluorescent quantitative real-time PCR assay for the rapid detection of Edwardsiella tarda. Xie Guosi,Huang Jie,Zhang Qingli,Han Nana,Shi Chengyin,Wang Xiuhua,Xie Guosi,Huang Jie. 2012

[17]Specific MHC class II B alleles associated with resistance to Edwardsiella tarda in turbot, Psetta maxima (L.). Xu, J-Y,Chen, S-L,Ding, H.,Xu, J-Y. 2009

[18]A Streptococcus iniae DNA vaccine delivered by a live attenuated Edwardsiella tarda via natural infection induces cross-genus protection. Sun, Y.,Hu, Y-H.,Sun, L.,Sun, Y.,Liu, C-S.. 2012

[19]A real-time PCR targeted to the upstream regions of HlyB for specific detection of Edwardsiella tarda. Xie Guosi,Huang Jie,Zhang Qingli,Han Nana,Shi Chengyin,Wang Xiuhua,Liu Qinghui,Xie Guosi,Huang Jie. 2012

[20]Generation and evaluation of virulence attenuated mutants of Edwardsiella tarda as vaccine candidates to combat edwardsiellosis in flounder (Paralichthys olivaceus). Li, Jie,Mo, Zhaolan,Li, Guiyang,Huang, Jie,Li, Jie,Mo, Zhaolan,Li, Guiyang,Huang, Jie,Xiao, Peng.

作者其他论文 更多>>

微信小程序