Influence of white spot syndrome virus infection on hepatopancreas gene expression of 'Huanghai No. 2' shrimp (Fenneropenaeus chinensis)

文献类型： 外文期刊

第一作者： Meng, Xianhong

作者： Meng, Xianhong;Shi, Xiaoli;Kong, Jie;Luan, Sheng;Luo, Kun;Cao, Baoxiang;Liu, Ning;Lu, Xia;Li, Xupeng;Deng, Kangyu;Cao, Jiawang;Zhang, Yingxue;Zhang, Hengheng;Meng, Xianhong;Shi, Xiaoli;Kong, Jie;Luan, Sheng;Luo, Kun;Cao, Baoxiang;Liu, Ning;Lu, Xia;Li, Xupeng;Deng, Kangyu;Cao, Jiawang;Zhang, Yingxue;Zhang, Hengheng

作者机构：

关键词： Huanghai No. 2;Fenneropenaeus chinensis;crustacean immunity;microarray;white spot syndrome virus

期刊名称：JOURNAL OF OCEAN UNIVERSITY OF CHINA （ 影响因子：0.913； 五年影响因子：1.012 ）

ISSN： 1672-5182

年卷期： 2017 年 16 卷 5 期

页码：

收录情况： SCI

摘要： To elucidate the molecular response of shrimp hepatopancreas to white spot syndrome virus (WSSV) infection, microarray was applied to investigate the differentially expressed genes in the hepatopancreas of 'Huanghai No. 2' (Fenneropenaeus chinensis). A total of 59137 unigenes were designed onto a custom-made 60K Agilent chip. After infection, the gene expression profiles in the hepatopancreas of the shrimp with a lower viral load at early (48-96 h), peak (168-192 h) and late (264-288 h) infection phases were analyzed. Of 18704 differentially expressed genes, 6412 were annotated. In total, 5453 differentially expressed genes (1916 annotated) expressed at all three phases, and most of the annotated were either up- or down-regulated continuously. These genes function diversely in, for example, immune response, cytoskeletal system, signal transduction, stress resistance, protein synthesis and processing, metabolism among others. Some of the immune-related genes, including antilipopolysaccharide factor, Kazal-type proteinase inhibitor, C-type lectin and serine protease encoding genes, were up-regulated after WSSV infection. These genes have been reported to be involved in the anti-WSSV responses. The expression of genes related to the cytoskeletal system, including beta-actin and myosin but without tubulin genes, were down-regulated after WSSV infection. Astakine was found for the first time in the WSSV-infected F. chinensis. To further confirm the expression of differentially expressed genes, quantitative real-time PCR was performed to test the expression of eight randomly selected genes and verified the reliability and accuracy of the microarray expression analysis. The data will provide valuable information to understanding the immune mechanism of shrimp's response to WSSV.

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