Molecular identification and functional analysis of Ctrp9 in Epinephelus coioides

文献类型: 外文期刊

第一作者: Li, Wensheng

作者: Li, Wensheng;Qin, Chaobin;Wang, Bin

作者机构:

关键词: Ctrp9;food intake;metabolism;grouper

期刊名称:JOURNAL OF MOLECULAR ENDOCRINOLOGY ( 影响因子:5.098; 五年影响因子:4.755 )

ISSN: 0952-5041

年卷期: 2017 年 58 卷 4 期

页码:

收录情况: SCI

摘要: CTRP9 is a member of the C1q/TNF-related protein (CTRP) superfamily and has been studied in mammals, whereas the comparative studies of CTRP9 in non-mammalian species are still absent. In this study, ctrp9 was isolated and characterized from the orange-spotted grouper (Epinephelus coioides). The full-length cDNA of ctrp9 was 1378 bp in size with an ORF (open reading frame) of 1020 bp that encodes a 339 amino acid pre-pro hormone. The mRNA expression of ctrp9 showed a rather high level in the kidney and brain, but a low level in other tissues. Furthermore, the mRNA expression of ctrp9 decreased significantly in the liver after fasting for 7 days and restored to the normal levels after refeeding. In contrast, the ctrp9 mRNA level increased in the hypothalamus after fasting. The recombinant gCtrp9 (globular Ctrp9) was prepared using the Pichia pastoris expression system and was verified by Western blot as well as mass spectrometry assays. In the primary hepatocytes culture, the recombinant gCtrp9 could inhibit the glucose production after 12-h treatment. After i.p. (intraperitoneal) injection with recombinant gCtrp9, in hypothalamus, mRNA expression levels of npy and orexin (orexigenic factors) decreased, whereas the expression levels of crh and pomc (anorexigenic factors) increased. Moreover, i.p. injection with the recombinant gCtrp9 could reduce the serum concentrations of glucose, TG and low-density lipoprotein cholesterol but increase the content of high-density lipoprotein cholesterol. Our studies for the first time unveil the structure of Ctrp9 and its potential role as a regulatory factor of metabolism and food intake in teleost.

分类号:

  • 相关文献

[1]Growth, feed intake and immune responses of orange-spotted grouper (Epinephelus coioides) exposed to low infectious doses of ectoparasite (Cryptocaryon irritans). Yin, Fei,Sun, Peng,Shi, Zhao-Hong,Gao, Quan-Xin,Peng, Shi-Ming,Dan, Xue-Ming,Li, An-Xing. 2014

[2]Effects of Cryptocaryon irritans infection on the survival, feeding, respiratory rate and ionic regulation of the marbled rockfish Sebastiscus marmoratus. Yin, Fei,Sun, Peng,Gao, Quanxin,Shi, Zhaohong,Peng, Shiming,Gong, Qiyang,Li, Yanwei,Li, Anxing,Dan, Xueming. 2014

[3]Food ingestion, prey selectivity, feeding incidence, and performance of yellowtail kingfish Seriola lalandi larvae under constant and varying temperatures. Ma, Zhenhua,Ma, Zhenhua. 2014

[4]Identification and characterization of a novel lymphocystis disease virus isolate from cultured grouper in China. Huang, X.,Huang, Y.,Wei, S.,Ouyang, Z.,Qin, Q.,Xu, L.,Feng, J..

[5]Diagnosis and ORF Gene Sequencing Analysis of The Nervous Necrosis Virus (NNV) Isolated from Cultured Pearl Gentian Grouper, Epinephelus lanceolatus x Epinephelus fuscoguttatus, in China. Ma, Rong-Rong,Wang, Yin-Geng,Liao, Mei-Jie,Zhang, Zheng,Rong, Xiao-Jun,Li, Bin,Ma, Rong-Rong,Yang, Xian-Le. 2014

[6]Isolation, identification and pathogenicity of Vibrio harveyi, the causal agent of skin ulcer disease in juvenile hybrid groupers Epinephelus fuscoguttatus x Epinephelus lanceolatus. Shen, G. M.,Shi, C. Y.,Fan, C.,Jia, D.,Wang, S. Q.,Xie, G. S.,Li, G. Y.,Mo, Z. L.,Huang, J.,Shen, G. M.,Shi, C. Y.,Fan, C.,Jia, D.,Wang, S. Q.,Xie, G. S.,Li, G. Y.,Mo, Z. L.,Huang, J.,Shen, G. M.,Fan, C.,Jia, D..

[7]Neuropeptide Y content in the hypothalamic paraventricular nucleus responds to fasting and refeeding in broiler chickens. Zhou, WD,Murakami, M,Hasegawa, S,Yoshizawa, F,Sugahara, K.

[8]High plant diversity stimulates foraging motivation in grazing herbivores. Feng, Chao,Ding, Shiwen,Zhang, Tianyuan,Li, Zhiqiang,Wang, Deli,Wang, Ling,Liu, Chen,Sun, Jinyan,Peng, Fugang.

[9]Mass Mortality of Cage-cultured Orange-spotted Grouper Epinephelus coioides Associated with Renal Sphaerosporosis Caused by Sphaerospora epinepheli in South China Sea. Xu, Li-Wen,Feng, Juan,Zhang, Jing-Yong,Wang, Jian-Guo.

[10]Ribavirin, a nucleoside with potential insecticidal activity. Liu, Ying-Qian,Li, Lin-Hai,Kou, Liang,Zhang, Jing,Feng, Gang,Yang, Liu.

[11]CA-4, a natural cis-stilbene compound with potential insecticidal activity. Lv, Xiu-Qing,Feng, Gang,Liu, Ying-Qian,Nan, Xiang,Yang, Liu.

[12]Identification of a UDP-glucose pyrophosphorylase from cotton (Gossypium hirsutum L.) involved in cellulose biosynthesis in Arabidopsis thaliana. Qinghua Wang,Xue Zhang,Fuguang Li,Yuxia Hou,Xingliang Liu,Xueyan Zhang.

[13]Changes in sugar content and relative enzyme activity in grape berry in response to root restriction. Xie, ZhaoSen,Li, Bo,Xu, WenPing,Wang, ShiPing,Li, Bo,Forney, Charles F.. 2009

[14]Analysis of weighted co-regulatory networks in maize provides insights into new genes and regulatory mechanisms related to inositol phosphate metabolism. Zhang, Shaojun,Yang, Wenzhu,Zhao, Qianqian,Zhou, Xiaojin,Jiang, Ling,Ma, Shuai,Liu, Xiaoqing,Li, Ye,Zhang, Chunyi,Fan, Yunliu,Chen, Rumei,Zhang, Shaojun,Yang, Wenzhu,Zhao, Qianqian,Zhou, Xiaojin,Jiang, Ling,Liu, Xiaoqing,Li, Ye,Zhang, Chunyi,Fan, Yunliu,Chen, Rumei. 2016

[15]Gene expression profiling reveals candidate genes related to residual feed intake in duodenum of laying ducks. Zeng, T.,Huang, L.,Ren, J.,Chen, L.,Tian, Y.,Lu, L.,Huang, L.,Huang, Y.,Zhang, H.,Du, J.. 2017

[16]The Eukaryote-Like Serine/Threonine Kinase STK Regulates the Growth and Metabolism of Zoonotic Streptococcus suis. Zhang, Chunyan,Sun, Wen,Dong, Mengmeng,Liu, Wanquan,Li, Lu,Xu, Zhuofei,Zhou, Rui,Tan, Meifang,Gao, Ting,Li, Lu,Xu, Zhuofei,Zhou, Rui. 2017

[17]Systematic identification of mitochondrial lysine succinylome in silkworm (Bombyx mori) midgut during the larval gluttonous stage. Chen, Jine,Li, Fengbo,Liu, Yan,Shen, Weifeng,Du, Xin,He, Lihua,Meng, Zhiqi,Wang, Yongqiang,Ma, Xianliang. 2018

[18]Zinc Oxide Nanoparticles Influence Microflora in Ileal Digesta and Correlate Well with Blood Metabolites. Feng, Yanni,Min, Lingjiang,Zhang, Weidong,Chu, Meiqiang,Li, Lan,Shen, Wei,Zhao, Yong,Zhang, Hongfu,Liu, Jing,Hou, Zhumei,Zhao, Yong. 2017

[19]Manipulation of Metabolic Pathways to Develop Vitamin-Enriched Crops for Human Health. Jiang, Ling,Wang, Weixuan,Lian, Tong,Zhang, Chunyi,Jiang, Ling,Wang, Weixuan,Zhang, Chunyi. 2017

[20]Regulation of protein metabolism by glutamine: implications for nutrition and health. Jiang, Zongyong,Zheng, Chuntian,Lin, Yingcai,Xi, Pengbin,Jiang, Zongyong,Zheng, Chuntian,Lin, Yingcai,Xi, Pengbin,Wu, Guoyao,Wu, Guoyao. 2011

作者其他论文 更多>>