Molecular identification and functional analysis of Ctrp9 in Epinephelus coioides
文献类型: 外文期刊
第一作者: Li, Wensheng
作者: Li, Wensheng;Qin, Chaobin;Wang, Bin
作者机构:
关键词: Ctrp9;food intake;metabolism;grouper
期刊名称:JOURNAL OF MOLECULAR ENDOCRINOLOGY ( 影响因子:5.098; 五年影响因子:4.755 )
ISSN: 0952-5041
年卷期: 2017 年 58 卷 4 期
页码:
收录情况: SCI
摘要: CTRP9 is a member of the C1q/TNF-related protein (CTRP) superfamily and has been studied in mammals, whereas the comparative studies of CTRP9 in non-mammalian species are still absent. In this study, ctrp9 was isolated and characterized from the orange-spotted grouper (Epinephelus coioides). The full-length cDNA of ctrp9 was 1378 bp in size with an ORF (open reading frame) of 1020 bp that encodes a 339 amino acid pre-pro hormone. The mRNA expression of ctrp9 showed a rather high level in the kidney and brain, but a low level in other tissues. Furthermore, the mRNA expression of ctrp9 decreased significantly in the liver after fasting for 7 days and restored to the normal levels after refeeding. In contrast, the ctrp9 mRNA level increased in the hypothalamus after fasting. The recombinant gCtrp9 (globular Ctrp9) was prepared using the Pichia pastoris expression system and was verified by Western blot as well as mass spectrometry assays. In the primary hepatocytes culture, the recombinant gCtrp9 could inhibit the glucose production after 12-h treatment. After i.p. (intraperitoneal) injection with recombinant gCtrp9, in hypothalamus, mRNA expression levels of npy and orexin (orexigenic factors) decreased, whereas the expression levels of crh and pomc (anorexigenic factors) increased. Moreover, i.p. injection with the recombinant gCtrp9 could reduce the serum concentrations of glucose, TG and low-density lipoprotein cholesterol but increase the content of high-density lipoprotein cholesterol. Our studies for the first time unveil the structure of Ctrp9 and its potential role as a regulatory factor of metabolism and food intake in teleost.
分类号:
- 相关文献
作者其他论文 更多>>
-
Dynamic changes of quality characteristics during fermentation of orange-fleshed sweet potato alcoholic beverage
作者:Wu, Huimin;Fu, Qinquan;Tang, Tiantian;Liu, Yinqi;Ma, Xinyi;Zhao, Min;Zhou, Xin;Wang, Bin;Shi, Xuewei;Yang, Yiling;Wang, Zhangying;Yang, Yiling;Wang, Zhangying;Wang, Bin;Shi, Xuewei;Wang, Bin;Shi, Xuewei;Wang, Bin;Shi, Xuewei
关键词:Orange-fleshed sweet potato (OFSP); Alcoholic beverage; Antioxidant properties; Physicochemical indicators; Volatile compounds
-
Effects of Different Light Spectra on Oxidative Stress and Nutritional Quality of the Fish Plectropomus leopardus
作者:Li, Wensheng;Zhang, Zheng;Liu, Baoliang;Fang, Yingying;Cao, Shuquan;Li, Wenyang;Fei, Fan;Li, Wensheng;Liu, Baoliang;Sun, Yan;He, Chengbin;Zhang, Chuanxin
关键词:light spectra; blood biochemistry; antioxidant stress; nutritional quality; Plectropomus leopardus; light spectra; blood biochemistry; antioxidant stress; nutritional quality; Plectropomus leopardus
-
In-situ constructing AlTiZn/CN heterojunction via memory effect for effective degradation of naphthalene under UV irradiation
作者:Liang, Jinhua;Fang, Yan;Li, Weikang;Yang, Tianyi;Peng, Chaofei;Liu, Boqing;Chen, Ke;Sun, Qiyuan;Wang, Bin;Ren, Xiaoqian;Xu, Wenlong
关键词:Self-assembly; 20/AlTiZn/CN heterojunction; Memory effect; Photodegradation; Naphthalene
-
Increased ABA synthesis by overexpression of Cd-NAC ameliorates cadmium toxicity in vegetable soybean
作者:Wang, Bin;Zhang, Guwen;Liu, Na;Feng, Zhijuan;Bu, Yuanpeng;Gong, Yaming;Fang, Ruiqiu;Wang, Bin;Zhang, Guwen;Liu, Na;Feng, Zhijuan;Bu, Yuanpeng;Gong, Yaming
关键词:Cadmium stress; Abscisic acid; Cd-NAC
-
Intercropping outweighs straw incorporation driving community and functional diversity of microarthropods after 5 years of tillage practices
作者:Liu, Yuhang;Gao, Qiang;Sun, Xin;Wang, Bin;Sun, Xin;Liu, Yuhang;Wang, Yongjun;Yao, Fanyun;Wang, Bin;Sun, Xin;Wu, Haitao;Sun, Xin
关键词:Maize-peanut intercropping; Microarthropods; Straw incorporation; Sustainable agriculture
-
Farmers' decisions on crop residues utilization, greenhouse gases reduction and subsidy of crop residue-based bioenergy: An agent-based life cycle model
作者:Zhang, Jiaqi;Zhang, Jiaqi;Wang, Bin;Li, Yu'e;Zhang, Jiaqi;Oosterveer, Peter;Zhuge, Chengxiang;Huang, Qitong;Huang, Qitong
关键词:Agent-based model; Crop residue-based bioenergy; Life cycle thinking; Environmental and economic impacts; Farmer decision making
-
A CCT protein GmCIC5 activates GmP5CS to regulate proline accumulation and cadmium tolerance in vegetable soybean
作者:Wang, Bin;Zhang, Guwen;Liu, Na;Feng, Zhijuan;Bu, Yuanpeng;Gong, Yaming;Fang, Ruiqiu;Wang, Bin;Zhang, Guwen;Liu, Na;Feng, Zhijuan;Bu, Yuanpeng;Gong, Yaming;Wang, Bin;Zhang, Guwen;Liu, Na;Feng, Zhijuan;Bu, Yuanpeng;Gong, Yaming
关键词:Vegetable soybean; Cadmium stress; Proline; GmCIC5