Development and validation of 89 novel expressed sequence tag-derived microsatellite markers in blood clam, Tegillarca granosa

文献类型： 外文期刊

第一作者： Teng, Shuangshuang

作者： Teng, Shuangshuang;Fang, Jun;Cai, Yilong;Chai, Xueliang;Xiao, Guoqiang;Teng, Shuangshuang;Fang, Jun;Cai, Yilong;Chai, Xueliang;Xiao, Guoqiang;Teng, Shuangshuang;Fang, Jun;Cai, Yilong;Chai, Xueliang;Xiao, Guoqiang

作者机构：

关键词： Tegillarca granosa;expressed sequence tag;microsatellite

期刊名称：JOURNAL OF OCEAN UNIVERSITY OF CHINA （ 影响因子：0.913； 五年影响因子：1.012 ）

ISSN： 1672-5182

年卷期： 2017 年 16 卷 3 期

页码：

收录情况： SCI

摘要： Blood clam, Tegillarca granosa, is an important shellfish in Chinese mariculture industry. Investigative research in this species, such as genetic linkage mapping, requires a large panel of molecular markers. In present study, a total of 89 polymorphic microsatellite markers were developed in T. granosa using the sequence database of Life Sciences Technology 454 next generation sequencing technology. All 89 loci were characterized in 20 individual clams from a natural population inhabiting Yueqing Gulf, Zhejiang Province, China. The number of alleles per polymorphic locus varied between 2 and 15, while the observed heterozygosity, expected heterozygosity and polymorphic information content varied between 0.000 and 1.000, 0.102 and 0.921, and 0.048 and 0.886, respectively. Of the 89 loci identified, 32 loci deviated significantly from Hardy-Weinberg equilibrium following Bonferroni correction. Thirty nine markers, which were shown to be polymorphic in a full-sibling family, were tested in Mendelian segregations. As expected, 32 loci were co-dominantly segregated in a Mendelian fashion. These novel developed microsatellite markers represent useful research tools for investigation of population genetic structure and genetic diversity in this species.

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