Expression Characteristics of beta-Catenin in Scallop Chlamys farreri Gonads and Its Role as a Potential Upstream Gene of Dax1 through Canonical Wnt Signalling Pathway Regulating the Spermatogenesis

文献类型： 外文期刊

第一作者： Li, Hailong

作者： Li, Hailong;Zhang, Zhifeng;Bi, Ying;Yang, Dandan;Zhang, Litao;Liu, Jianguo;Li, Hailong

作者机构：

期刊名称：PLOS ONE （ 影响因子：3.24； 五年影响因子：3.788 ）

ISSN： 1932-6203

年卷期： 2014 年 9 卷 12 期

页码：

收录情况： SCI

摘要： beta-catenin is a key signaling molecule in the canonical Wnt pathway, which is involved in animal development. However, little information has been reported for beta-catenin in bivalves. In the present study, we cloned a homolog of beta-catenin from the scallop Chlamys farreri and determined its expression characteristics. The full-length cDNA of beta-catenin was 3,353 bp, including a 2,511 bp open reading frame that encoded a predicted 836 amino acid protein. Level of the beta-catenin mRNA increased significantly (P<0.05) with C. farreri gonadal development and presented a sexually dimorphic expression pattern in the gonads, which was significantly high in ovaries detected by quantitative real-time polymerase chain reaction (qRT-PCR). Immunohistochemical analysis revealed that the beta-catenin was mainly located in germ cells of the gonads, with obvious positive immune signals in the oogonia and oocytes of ovaries as well as in the spermatogonia and spermatocytes of testes, implying beta-catenin might be involved in the gametogenesis of C. farreri. Furthermore, when 0.1 mu g/mL and 0.2 mu g/mL DKK-1 (an inhibitor of the canonical Wnt pathway) were added in vitro to culture medium containing testis cells of C. farreri, the expression of beta-catenin decreased significantly detected by qRT-PCR (P<0.05), suggesting the canonical Wnt signal pathway exists in the scallop testis. Similarly, when 50 mu M and 100 mu M quercetin (an inhibitor of beta-catenin) were added in vitro to the culture system, Dax1 expression was significantly down-regulated compared with controls (P<0.05), implying the beta-catenin is an upstream gene of Dax1 and is involved in the regulation of C. farreri spermatogenesis.

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