Transcriptome analysis gene expression in the liver of Coilia nasus during the stress response

文献类型： 外文期刊

第一作者： Du, Fukuan

作者： Du, Fukuan;Xu, Gangchun;Nie, Zhijuan;Xu, Pao;Gu, Ruobo

作者机构：

关键词： Gene expression;Coilia nasus;Stress;Liver injury;Apoptosis

期刊名称：BMC GENOMICS （ 影响因子：3.969； 五年影响因子：4.478 ）

ISSN： 1471-2164

年卷期： 2014 年 15 卷

页码：

收录情况： SCI

摘要： Background: The estuarine tapertail anchovy (Coilia nasus) is widely distributed in the Yangtze River, the coastal waters of China, Korea, and the Ariake Sound of Japan. It is a commercially important species owing to its nutritional value and delicate flavor. However, Coilia nasus is strongly responsive to stress, this often results in death, which causes huge losses. In this study, we used next-generation sequencing technologies to study changes in gene expression in response to loading stress and the mechanism of death caused by loading stress in Coilia nasus. Results: Using next-generation RNA-seq technologies on an Illumina HiSeq 2000 platform, we assembled a de novo transcriptome and tested for differential expression in response to stress. A total of 65,129 unigenes were generated, the mean unigene size and N50 were 607 bp and 813 bp, respectively. Of the assembled unigenes, we identified 2,990 genes that were significantly up-regulated, while 3,416 genes were significantly down-regulated in response to loading stress. Pathway enrichment analysis based on loading stress-responsive unigenes identified significantly stress related pathways. "Metabolism" and "immunity" were the two most frequently represented categories. In the "metabolism" category, "glucose metabolism" and "lipid metabolism" were major subclasses. The transcriptional expression of rate-limiting enzymes in "glucose metabolism" and "lipid metabolism" was detected by RT-qPCR, all were significantly increased after stress. Apoptosis associated proteins tumor necrosis factor alpha (TNF-alpha), caspase 9, cytochrome c and caspase 3 in the stress group were significantly elevated, moreover, liver injury indicators (Alanine aminotransferase, ALT, and aspartate transaminase, AST) were also significantly elevated, which indicates that loading stress induced liver injury. Conclusion: This study provided abundant unigenes that could contribute greatly to the discovery of novel genes in fish. The alterations in predicted gene expression patterns reflected possible responses to stress. Loading stress may induce liver injury through the mitochondrial apoptosis pathway, which was activated by TNF-alpha. Taken together, our data not only provide information that will aid the identification of novel genes from fish, but also shed new light on the understanding of mechanisms by which physical stressors cause death in fish.

分类号：

  • 相关文献

[1]Anti-inflammatory and hepatoprotective effects of Ganoderma lucidum polysaccharides on carbon tetrachloride-induced hepatocyte damage in common carp (Cyprinus carpio L.). Liu, Ying-Juan,Jia, Rui,Shen, Yu-Jin,Zhao, Cai-Yuan,Yin, Guo-Jun,Du, Jin-Liang,Cao, Li-Ping,Xu, Pao,Yin, Guo-Jun,Du, Jin-Liang,Cao, Li-Ping,Xu, Pao,Yin, Guo-Jun. 2015

[2]Molecular characterization and differential expression of the myostatin gene in Coilia nasus. Du, Fukuan,Xu, Gangchun,Nie, Zhijuan,Xu, Pao,Gu, Ruobo.

[3]Molecular characterization of the soybean L-asparaginase gene induced by low temperature stress. Cho, Chang-Woo,Lee, Hye-Jeong,Chung, Eunsook,Kim, Kyoung Mi,Kim, Jee Eun Heo Jung-In,Chung, Jongil,Ma, Youzhi,Fukui, Kiichi,Lee, Dae-Won,Kim, Doh-Hoon,Chung, Young-Soo,Lee, Jai-Heou. 2007

[4]Cloning and expression of heat shock protein genes in two whitefly species in response to thermal stress. Wan, F. -H.,Yu, H.,Wan, F. -H..

[5]Transcriptome analysis and discovery of genes involved in immune pathways in large yellow croaker (Larimichthys crocea) under high stocking density stress. Sun, Peng,Bao, Peibo,Tang, Baojun,Bao, Peibo.

[6]Effects of dietary resveratrol supplementation on hepatic and serum pro-/anti-inflammatory activity in juvenile GIFT tilapia, Oreochromis niloticus. Zheng, Yao,Wu, Wei,Song, Chao,Meng, Shunlong,Fan, Limin,Bing, Xuwen,Chen, Jiazhang,Zheng, Yao,Zhao, Zhixiang,Wu, Wei,Bing, Xuwen,Chen, Jiazhang.

[7]The p53-Bak apoptotic signaling axis plays an essential role in regulating differentiation of the ocular lens. Deng, M.,Ji, W.,Zhang, X.,Gong, L.,Hu, X.,Hu, W.,Xiao, L.,Li, D. W. -C.,Chen, P.,Liu, F.,Fu, S.,Tang, H.,Fu, Y.,Xiong, Z.,Hui, S.,Hu, X.,Hu, W.,Xiao, L.,Liu, W. -B.,Xiao, Y. -M.,Liu, S. -J.,Liu, Y.,Li, D. W. -C.,Zhang, L.,Sun, S.,Liu, J.,Li, D. W. -C..

[8]Molecular and immune response characterizations of a novel Bax inhibitor-1 gene in pufferfish, Takifugu obscurus. Cheng, Chang-Hong,Guo, Zhi-Xun,Luo, Sheng-Wei,Wang, An-Li,Guo, Zhi-Xun.

[9]Comparison of the developmental competence and quality of bovine embryos obtained by in vitro fertilization with sex-sorted and unsorted semen from seven bulls. Liu, Xia,Hu, Tingxi,Sun, Weijun,Hao, Haisheng,Liu, Yan,Zhao, Xueming,Zhu, Huabin,Du, Weihua,Hu, Tingxi.

[10]BIRC7 gene in channel catfish (Ictalurus punctatus): Identification and expression analysis in response to Edwardsiella tarda, Streptococcus iniae and channel catfish Hemorrhage Reovirus. Li, Min,Wang, Qi-Long,Chen, Song-Lin,Sha, Zhen-Xia,Li, Min,Liu, Yang.

[11]THE PROTECTIVE EFFECTS OF CASSAVA (MANIHOT ESCULENTA CRANTZ) LEAF FLAVONOID EXTRACTS ON LIVER DAMAGE OF CARBON TETRACHLORIDE INJURED MICE. Tao, Hai-Teng,Qiu, Bin,Du, Fang-Ling,Xu, Tong-Cheng,Liu, Li-Na,Liu, Wei,Lu, Feijie,Li, Kai-Mian. 2015

[12]Protective effect of Ganoderma lucidum polysaccharide against carbon tetrachloride-induced hepatic damage in precision-cut carp liver slices. Liu, Yingjuan,Zhang, Chunyun,Yin, Guojun,Du, Jinliang,Jia, Rui,Cao, Liping,Xu, Pao,Yin, Guojun,Du, Jinliang,Jia, Rui,Cao, Liping,Xu, Pao,Yin, Guojun,Jeney, Galina,Teraoka, Hiroki.

[13]A STUDY ON EFFECTS OF GLUTATHIONE S-TRANSFERASE FROM SILKWORM ON CCL4-INDUCED MOUSE LIVER INJURY. Yan, Hui,Cui, Zhongzheng,Wang, Bochu,Cui, Zhongzheng,Wang, Bochu. 2011

[14]Preliminary characterization, antioxidant activity in vitro and hepatoprotective effect on acute alcohol-induced liver injury in mice of polysaccharides from the peduncles of Hovenia dulcis. Wang, Mingchun,Jiang, Changxing,Ma, Liping,Zhang, Zhanjun,Zeng, Xiaoxiong,Zhu, Peilei. 2012

[15]The protective effect of silymarin on the carbon tetrachloride (CCl4)-induced liver injury in common carp (Cyprinus carpio). Jia, Rui,Cao, Liping,Du, Jinliang,Xu, Pao,Yin, Guojun,Jeney, Galina. 2013

[16]Developmental Expression of HSP60 and HSP10 in the Coilia nasus Testis during Upstream Spawning Migration. Fang, Di-An,Zhou, Yan-Feng,Zhang, Min-Ying,Xu, Dong-Po,Liu, Kai,Duan, Jin-Rong,Fang, Di-An,Xu, Dong-Po. 2017

[17]Molecular Characteristic, Protein Distribution and Potential Regulation of HSP90AA1 in the Anadromous Fish Coilia nasus. Fang, Di-An,Duan, Jin-Rong,Zhou, Yan-Feng,Zhang, Min-Ying,Xu, Dong-Po,Liu, Kai,Xu, Pao,Fang, Di-An,Xu, Dong-Po. 2016

[18]First studies of embryonic and larval development of Coilia nasus (Engraulidae) under controlled conditions. Xu, Gangchun,Tang, Xue,Gu, Ruobo,Xu, Pao,Tang, Xue,Le, Guowei,Zhang, Chengxiang,Zheng, Jinliang. 2011

[19]Screening potential SSR markers of the anadromous fish Coilia nasus by de novo transcriptome analysis using Illumina sequencing. Fang, D. -A.,Xu, P.,Fang, D. -A.,Zhou, Y. -F.,Duan, J. -R.,Zhang, M. -Y.,Xu, D. -P.,Liu, K.,Xu, P.,Wei, Q.. 2015

[20]Differences in muscle cellularity and flesh quality between wild and farmed Coilia nasus (Engraulidae). Tang, Xue,Xu, Gangchun,Xu, Pao,Gu, Ruobo,Tang, Xue,Dai, Hui,Zhang, Chengxiang. 2012

作者其他论文 更多>>

微信小程序