scFv antibodies against infectious bursal disease virus isolated from a combinatorial antibody library by flow cytometry

文献类型： 外文期刊

第一作者： Xu, Li-Ming

作者： Xu, Li-Ming;Li, Tian-He;Zhou, Bing;Guo, Mo;Liu, Miao;Zhao, Jing-Zhuang;Li, De-Shan;Cao, Hong-Wei

作者机构：

关键词： Antibody library;Bacterial display library;Bursal disease virus;Infectious bursal disease virus;Spheroplasts;Viral proteins;VP2 protein

期刊名称：BIOTECHNOLOGY LETTERS （ 影响因子：2.461； 五年影响因子：2.457 ）

ISSN： 0141-5492

年卷期： 2014 年 36 卷 5 期

页码：

收录情况： SCI

摘要： Infectious bursal disease is an economically important disease that affects chickens worldwide. Here, a recombinant single chain variable fragment (scFv) antibody library derived from chickens immunized with VP2 protein of infectious bursal disease virus (IBDV) was constructed. The library was subjected to three rounds of screening by flow cytometry against VP2 protein through a bacteria display technology, resulting in the enrichment of scFv. Three scFv clones with different fluorescence intensity were obtained by random colony pick up. The isolated scFv antibodies were expressed and purified. Relative affinity assay showed the three clones had different sensitivity to VP2, in accordance with fluorescence activity cell sorting analysis. The potential use of the selected IBDV-specific scFv antibodies was demonstrated by the successful application of the isolated antibodies in western blotting assay and ELISA.

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