Characterization of ribulose-1, 5-bisphosphate carboxylase/oxygenase and transcriptional analysis of its related genes in Saccharina japonica (Laminariales, Phaeophyta)

文献类型： 外文期刊

第一作者： Shao Zhanru

作者： Shao Zhanru;Li Qiuying;Yao Jianting;Duan Delin;Liu Fuli;Shao Zhanru;Li Qiuying

作者机构：

关键词： Saccharina japonica;Rubisco;photosynthetic genes;transcription;protein expression;carboxylase activity

期刊名称：CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY （ 影响因子：1.068； 五年影响因子：0.983 ）

ISSN： 0254-4059

年卷期： 2014 年 32 卷 2 期

页码：

收录情况： SCI

摘要： Saccharina japonica is a common macroalga in sublittoral communities of cold seawater environments, and consequently may have highly efficient ribulose-1, 5-bisphosphate carboxylase/oxygenase (Rubisco) activity for carbon assimilation. In our study, we cloned the full-length Rubisco gene from S. japonica (SJ-rbc). It contained an open reading frame for a large subunit gene (SJ - rbcL) of 1 467 bp, a small subunit gene (SJ-rbcS) of 420 bp, and a SJ-rbcL/S intergenic spacer of 269 bp. The deduced peptides of SJ-rbcL and SJ-rbcS were 488 and 139 amino acids with theoretical molecular weights and isoelectric points of 53.97 kDa, 5.81 and 15.84 kDa, 4.71, respectively. After induction with 1 mmol/L isopropyl-beta-D-thiogalactopyranoside for 5 h and purification by Ni2+ affinity chromatography, electrophoresis and western blot detection demonstrated successful expression of the 55 kDa SJ-rbcL protein. Real-time quantitative PCR showed that the mRNA levels of SJ-rbcL in gametophytes increased when transferred into normal growth conditions and exhibited diurnal variations: increased expression during the day but suppressed expression at night. This observation implied that Rubisco played a role in normal gametophytic growth and development. In juvenile sporophytes, mRNA levels of SJ-rbcL, carbonic anhydrase, Calvin-Benson-Bassham cycle-related enzyme, and chloroplast light-harvesting protein were remarkably increased under continuous light irradiance. Similarly, expression of these genes was up-regulated under blue light irradiance at 350 mu mol/(m(2)center dot s). Our results indicate that long-term white light and short-term blue light irradiance enhances juvenile sporophytic growth by synergistic effects of various photosynthetic elements.

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