Isolation and characterization of 57 novel polynucleotide microsatellites from yellow catfish (Pelteobagrus fulvidraco) genome for genetic analysis

文献类型： 外文期刊

第一作者： Shan, Yun-Jing

作者： Shan, Yun-Jing;Lu, Cui-Yun;Li, Chao;Cheng, Lei;Sun, Xiao-Wen;Shan, Yun-Jing

作者机构：

关键词： Pelteobagrus fulvidraco;Microsatellite;Genetic analysis

期刊名称：CONSERVATION GENETICS RESOURCES （ 影响因子：0.973； 五年影响因子：0.946 ）

ISSN： 1877-7252

年卷期： 2014 年 6 卷 1 期

页码：

收录情况： SCI

摘要： Yellow catfish (Pelteobagrus fulvidraco) is one of the important economic freshwater fish in Eastern Asia. In recent years, the wild resources were decreased sharply due to overfishing and pollution. It is necessary to conserve the wild stocks for the sustainable use of the species. In this study, 200 microsatellite markers were isolated from yellow catfish genome using Roche 454 pyrosequencing method. Among these markers, 57 exhibited polymorphism in yellow catfish population collected from Songhua River in China. The number of alleles ranged from 2 to 13 and the number of effect alleles ranged from 1.112 to 8.980. The observed heterozygosity (H (o)) and the expected heterozygosity (H (e)) varied from 0.106 to 0.957 and from 0.102 to 0.898, respectively. Most loci conformed to Hardy-Weinberg equilibrium with the exclusion of five loci. These polymorphic loci will be valuable for population genetic structure and genetic conservation for the P. fulvidraco.

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