IFN-gamma in turtle: Conservation in sequence and signalling and role in inhibiting iridovirus replication in Chinese soft-shelled turtle Pelodiscus sinensis

文献类型： 外文期刊

第一作者： Fu, Jian Ping

作者： Fu, Jian Ping;Chen, Shan Nan;Zou, Peng Fei;Guo, Zheng;Nie, Pin;Fu, Jian Ping;Huang, Bei;Nie, Pin;Zeng, Ling Bing;Qin, Qi Wei

作者机构：

关键词： IFN-gamma;IFN-gamma stimulated gene;Iridovirus;Antiviral activity;Turtle

期刊名称：DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY （ 影响因子：3.636； 五年影响因子：3.654 ）

ISSN： 0145-305X

年卷期： 2014 年 43 卷 1 期

页码：

收录情况： SCI

摘要： The IFN-gamma gene was identified in a turtle, the Chinese soft-shelled turtle, Pelodiscus sinensis, with its genome consisting of 4 exons and 3 introns. The deduced amino acid sequence of this gene contains a signal peptide, an IFN-gamma family signature motif (130)IQRKAVNELFPT, an NLS motif (KRKR)-K-155 and three potential N-glycosylation sites. As revealed by real-time quantitative PCR, the gene was constitutively expressed in all tested organs/tissues, with higher level observed in blood, intestine and thymus. An induced expression of IFN-gamma at mRNA level was observed in peripheral blood leucocytes (PBLs) in response to in vitro stimulation of LPS and PolyI:C. The overexpression of IFN-gamma in the Chinese soft-shelled turtle artery (STA) cell line resulted in the increase in the expression of transcriptional regulators, such as IRF1, IRF7 and STAT1, and antiviral genes, such as Mx, PKR, implying possibly the existence of a conserved signalling network and role for IFN-gamma in the turtle. Furthermore, the infection of soft-shelled turtle iridovirus (STIV) in the cell line transfected with IFN-gamma may cause the cell death as demonstrated with the elevated lactate dehydrogenase (LDH) level and cell mortality. However, the mechanism involved in the antiviral activity may require further investigation. (C) 2013 Elsevier Ltd. All rights reserved.

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