Molecular characterization of thioredoxin-1 and thioredoxin reductase activity in mud crab Scylla paramamosain

文献类型： 外文期刊

第一作者： Hu, J. H.

作者： Hu, J. H.;Zhang, F. Y.;Jiang, K. J.;Fang, Y. B.;Wang, J.;Zhao, M.;Qiao, Z. G.;Ma, L. B.;Hu, J. H.;Fang, Y. B.;Wang, J.;Zhao, M.

作者机构：

关键词： Scylla paramarnosain;Thioredoxin reductasc activity;Thiorcdoxin reductase;Thioredoxin-1 gene;Tissue expression;Quantitative real-time polymerase chain reaction

期刊名称：GENETICS AND MOLECULAR RESEARCH （ 影响因子：0.764； 五年影响因子：0.912 ）

ISSN： 1676-5680

年卷期： 2014 年 13 卷 4 期

页码：

收录情况： SCI

摘要： The thioredoxin (Trx) system consists of thioredoxin reductase (TrxR), Trx, and nicotinamide adenine dinucleotide phosphate (NADPH). TrxR is an NADPH-dependent oxidoreductase. Trx is a ubiquitous small protein with a redox-active disulfide bridge that plays important regulatory roles in some vital metabolic reactions. In this study, a cDNA sequence (SpTrx1) showing high identity to the first Trx gene was isolated from a hepatopancreas cDNA library of the mud crab Scylla paramamosain. The full-length cDNA of SpTrx1 consisted of 672 bp and contained a complete open reading frame of 318 bp encoding a polypeptide of 105 amino acids. Quantitative real-time polymerase chain reaction analysis revealed that SpTrx1 expression was ubiquitous in various organs of S. paramamosain, including the gill, muscle, heart, hemolymph, testis, and hepatopancrcas. SpTrx1 expression was upregulated significantly after Vibrio parahaemolyticus challenge: it obviously rose at 48 h and reached the highest level at 72 h. Furthermore. TrxR activity was detected in the gill, heart, muscle, hemolymph, and hepatopancreas. The relative TrxR activity in different tissues after V parahaemolyticus injection had the same tendency in each tissue (P <001) as SPTrx1 expression. The TrxR activity increased 2 h after injection, peaked at 8 h, slowly decreased from 12 to 24 h, and returned to normal levels at 48 h. The consistency of the expression between the TrX transcript and TrxR activity demonstrated that Trx was closely related to TrxR in the Trx system in S. paramamosain, suggesting that it may participate in the immune system of mud crabs.

分类号：

  • 相关文献

[1]A novel splice variant of the bovine GALNTL5 gene identified in Chinese Holstein bull testis tissue and its mRNA expression. Li, H.,Jiang, Q.,Ju, Z. H.,Huang, J. M.,Wang, X. G.,Yang, C. H.,Sun, Y.,Zhong, J. F.,Wang, C. F.,Li, H.,Yang, G. W.. 2016

[2]Identification and characterization of a novel splice variant of the PLC zeta 1 gene in bull testis tissues. Ju, Z. H.,Pan, Q.,Zhang, Y.,Huang, J. M.,Qi, C.,Wang, X. G.,Li, Q. L.,Zhong, J. F.,Wang, C. F.,Pan, Q.,Liu, M.. 2014

[3]Characterization of a TIR-NBS-LRR gene associated with downy mildew resistance in grape. Fan, J. J.,Xu, X.,Ruan, Y. Y.,Zhu, Y. S.,Cui, Z. H.,Zhang, L. J.,Wang, P.,Liu, K.. 2015

[4]Characterization, molecular cloning, and expression analysis of Ecsit in the spinyhead croaker, Collichthys lucidus. Song, W.,Jiang, K. J.,Zhang, F. Y.,Wang, J.,Ma, L. B.. 2016

[5]Molecular cloning, tissue expression of gene Muc2 in blunt snout bream Megalobrama amblycephala and regulation after re-feeding. Xue Chunyu,Xi Bingwen,Ren Mingchun,Xie Jun,Xu Pao,Xue Chunyu,Dong Jingjing,Xie Jun,Xi Bingwen. 2015

[6]Molecular identification of differential expression genes associated with sex pheromone biosynthesis in Spodoptera exigua. Zhang, Ya-Nan,Li, Jin-Bu,Zhu, Xiu-Yun,Zhang, Long-Wa,Chen, Da-Song,Sun, Liang,Li, Zhao-Qun,Ye, Zhan-Feng,Zheng, Mei-Yan.

[7]Candidate chemosensory genes identified in Colaphellus bowringi by antennal transcriptome analysis. Li, Xiao-Ming,Zhu, Xiu-Yun,Wang, Zhi-Qiang,Wang, Yi,Chen, Geng,Deng, Dao-Gui,Zhang, Ya-Nan,He, Peng,Sun, Liang. 2015

[8]Identification and Expression Analysis of Multiple Ferric Chelate Reductases in Citrus junos. Yao, Li-Xiao,He, Yong-Rui,Fan, Hai-Fang,Xu, Lan-Zhen,Lei, Tian-Gang,Zou, Xiu-Ping,Peng, Ai-Hong,Li, Qiang,Chen, Shan-Chun.

[9]Identification and comparison of candidate odorant receptor genes in the olfactory and non-olfactory organs of Holotrichia oblita Faldermann by transcriptome analysis. Wei, Hongshuang,Shu, Changlong,Zhang, Shuai,Cao, Yazhong,Yin, Jiao,Luo, Chen. 2017

[10]Cloning and characterization of type II interleukin-1 receptor cDNA from Japanese flounder (Paralichthys olivaceus). Fan, Yuding,Qi, Jie,Zhong, Qiwang,Zhang, Quanqi,Fan, Yuding,Zeng, Lingbing,Li, Shuo. 2010

[11]Tissue expression, association analysis between three novel SNPs of the RXR alpha gene and growth traits in Chinese indigenous cattle. Ma Yun,Gao HanTing,Chen NingBo,Xu YongJie,Jiang JinHang,Li Fen,Lu FangRu,Zhao Man,Gao HanTing,Lin Feng,Jiang JinHang,Shi KuiLin,Cheng Ni,Li JunYa. 2013

[12]Characterization and differential expression analysis of Toxocara canis aquaporin-1 gene. Luo, Yong-Fang,Hu, Ling,Ma, Guang-Xu,Luo, Yong-Li,Yin, Sha-Sha,Xiong, Yi,Zhou, Rong-Qiong,Zhu, Xing-Quan.

[13]Macrophage Migration Inhibitory Factor in Mud Crab Scylla paramamosain: Molecular Cloning, Expression Profiles in Various Tissues and under Vibrio challenge. Fang, Y.,Jiang, K.,Zhang, F.,Sun, M.,Hu, J.,Ma, L.,Fang, Y.,Sun, M.,Hu, J..

[14]Cloning and characterization of an invertebrate type lysozyme from Venerupis philippinarum. Zhao, Jianmin,Chen, Aiqin,Wu, Huifeng,Li, Chenghua,Qiu, Lihua,Ning, Xuanxuan.

[15]Molecular cloning, characterization and evolutionary analysis of vitellogenin in Chinese giant salamander Andrias davidianus. Tian, Hai-feng,Meng, Yan,Hu, Qiao-mu,Xiao, Han-bing,Hu, Qiao-mu.

[16]Characterization and Functional Analysis of Voltage-Dependent Anion Channel 1 (VDAC1) from Orange-Spotted Grouper (Epinephelus coioides). Shi, Yan,Hong, Xiaoyou,Chen, Kunci,Zhu, Xinping,Zhao, Zhe.

[17]Evidence that ghrelin may be associated with the food intake of gibel carp (Carassius auratus gibelio). Zhou, Chaowei,Zheng, Jinfa,Lei, Luo,Zhu, Chengke,Ye, Hua,Wang, Dan,Yang, Minmin,Wu, Jingjing,Zhu, Long,Zeng, Benhe,Yuan, Dengyue,Zhang, Chi.

作者其他论文 更多>>

微信小程序