Lipopolysaccharide and beta-1,3-glucan binding protein in the hard clam (Meretrix meretrix): Molecular characterization and expression analysis

文献类型： 外文期刊

第一作者： Liu, S. X.

作者： Liu, S. X.;Zhang, J. J.;Li, H. J.;Qi, Z. H.;He, C. B.;Gao, X. G.

作者机构：

关键词： Meretrix meretrix;Hard clam;Gene cloning;Lipopolysaccharide and beta-1,3-glucan binding protein;mRNA expression

期刊名称：GENETICS AND MOLECULAR RESEARCH （ 影响因子：0.764； 五年影响因子：0.912 ）

ISSN： 1676-5680

年卷期： 2014 年 13 卷 3 期

页码：

收录情况： SCI

摘要： Pattern recognition molecules play an important role in innate immunity by recognizing conserved molecular patterns that are present on the surface of invading microorganisms. In this study, a lipopolysaccharide and beta-1,3-glucan binding protein (LGBP) gene was cloned from the hard clam Meretrix meretrix (designated as Mm-LGBP) by the expressed sequence tags and rapid amplification of cDNA ends method. The cDNA was 1827 bp in length, consisting of a 71-bp 5'-terminal untranslated region, a 62-bp 3'UTR, and a 1734-bp open reading frame encoding a 577-amino acid polypeptide with an estimated molecular mass of 60.7 kDa and a theoretical isoelectric point of 5.56. Characteristic potential polysaccharide binding, cell adhesion, and glucanase motifs were identified in the Mm-LGBP, indicating that Mm-LGBP should be a new member of the LGBP family. Quantitative real-time polymerase chain reaction was developed to detect the mRNA expression level of Mm-LGBP in 6 different tissues. Higher-level mRNA expression of Mm-LGBP was detected in the gill and digestive gland tissues. The upregulation of Mm-LGBP mRNA after Vibrio anguillarum challenge showed that Mm-LGBP play a pivotal role in antibacterial immunity.

分类号：

  • 相关文献

[1]Molecular cloning and expression analysis of interleukin-1 beta from Japanese sea perch (Lateolabrax japonicus). Qiu, LH,Song, LS,Wu, LT,Cai, ZH,Jiang, SG. 2006

[2]Growth performance of the clam, Meretrix meretrix, breeding-selection populations cultured in different conditions. Wang Chao,Wang Hongxia,Liu Baozhong,Chai Xueliang,Tang Baojun. 2013

[3]Characterization of a long-chain fatty acid-CoA ligase 1 gene and association between its SNPs and growth traits in the clam Meretrix meretrix. Dai, Ping,Huan, Pin,Wang, Hongxia,Liu, Baozhong,Dai, Ping,Lu, Xia.

[4]The role of Cu/Zn-SOD and Mn-SOD in the immune response to oxidative stress and pathogen challenge in the clam Meretrix meretrix. Lu, Xia,Wang, Chao,Liu, Baozhong.

[5]Mining and identification of SNP markers associated with growth traits in the clam Meretrix meretrix. Dai, Ping,Dai, Ping,Wang, Hongxia,Liu, Baozhong,Liu, Baozhong.

[6]Cloning, characterization and expression of ferritin subunit from clam Meretrix meretrix in different larval stages. Wang, Xiaomei,Liu, Baozhong,Xiang, Jianhai,Wang, Xiaomei.

[7]Cloning and sequence analysis of putative type II fatty acid synthase genes from Arachis hypogaea L.. Li, Meng-Jun,Li, Ai-Qin,Xia, Han,Zhao, Chuan-Zhi,Li, Chang-Sheng,Wan, Shu-Bo,Bi, Yu-Ping,Wang, Xing-Jun. 2009

[8]Identification, cloning and characterization of a novel Gossypium hirsutum L. GhMYBantiV transcription factor in response to biotic and abiotic stresses. Li Fei,Chen Xiaoyan,He Xiaohong,Tang Ming,Hong Kun,Yang Zhengting,Yi Yin,Liu Zhanji,Fu Mingchuan. 2017

[9]Molecular Cloning and Characterization of a New Cold-active Extradiol Dioxygenase from a Metagenomic Library Derived from Polychlorinated Biphenyl-contaminated Soil. Ren He-jun,Zhou Rui,Wang Yan,Zhang Lan-ying,Lu Yang,Dai Chun-yan. 2012

[10]Characterization and cloning of SMALL GRAIN 4, a novel DWARF11 allele that affects brassinosteroid biosynthesis in rice. Shi, Zhenyuan,Rao, Yuchun,Xu, Jie,Hu, Shikai,Fang, Yunxia,Yu, Haiping,Pan, Jiangjie,Liu, Ruifang,Ren, Deyong,Wang, Xiaohu,Zhu, Yangzhou,Zhu, Li,Dong, Guojun,Zhang, Guangheng,Zeng, Dali,Guo, Longbiao,Hu, Jiang,Qian, Qian,Rao, Yuchun,Zhu, Yangzhou,Xu, Jie. 2015

[11]Molecular characterization, expression and activity of glucokinase in golden pompano, Trachinotus ovatus: Response of its expression to carbohydrate in the diet. Zhou, Chuanpeng,Wang, Jun,Wang, Yun,Lin, Heizhao,Huang, Zhong,Yu, Wei. 2018

[12]Cloning and expression of gynecophoral canal protein gene of Schistosoma japonicum (Chinese strain). Jin, YM,Lin, JJ,Feng, XG,Zhang, L,Wu, XF,Zhou, YC,Cai, YM. 2002

[13]The Research on Actinidia eriantha Benth. in South China. Wu, Y. J.,Xie, M.,Zhang, Q. C.,Zhang, H. Q.,Fang, J. B.. 2011

[14]VfCPK1, a gene encoding calcium-dependent protein kinase from Vicia faba, is induced by drought and abscisic acid. Liu, Guanshan,Chen, Jia,Wang, Xuechen. 2006

[15]Cloning and expression of actin gene of Schistosoma japonicum Chinese strain. Zhu, JG,Lin, JJ,Feng, XG,Wu, XF,Zhou, YC,Cai, YM. 2000

[16]Molecular responses of calreticulin gene to Vibrio anguillarum and WSSV challenge in the ridgetail white prawn Exopalaemon carinicauda. Duan, Yafei,Liu, Ping,Li, Jitao,Li, Jian,Chen, Ping,Duan, Yafei,Wang, Yun. 2014

[17]MULTI-TILLERING DWARF1, a new allele of BRITTLE CULM 12, affects plant height and tiller in rice. Yu, Haiping,Zhu, Yangzhou,Xu, Jiangmin,Lu, Mei,Ma, Bojun,Rao, Yuchun,Yu, Haiping,Ren, Deyong,Zhu, Yangzhou,Wang, Yuexing,Liu, Ruifang,Fang, Yunxia,Shi, Zhenyuan,Pan, Jiangjie,Hu, Jiang,Rao, Yuchun,Yu, Haiping,Yu, Haiping. 2016

[18]A novel functional gene associated with cold tolerance at the seedling stage in rice. Zhao, Junliang,Zhang, Shaohong,Dong, Jingfang,Yang, Tifeng,Mao, Xingxue,Liu, Qing,Wang, Xiaofei,Liu, Bin,Zhao, Junliang,Zhang, Shaohong,Dong, Jingfang,Yang, Tifeng,Mao, Xingxue,Liu, Qing,Wang, Xiaofei,Liu, Bin. 2017

[19]Cloning of the Bacillus thuringiensis serovar sotto chitinase (Schi) gene and characterization of its protein. Fang, Ji-Chao,Cai, Ping-Zhong,Yan, Wen-Zhao,Wu, Jie,Guo, Hui-Fang. 2005

[20]The chitinase C gene PsChiC from Pseudomonas sp and its synergistic effects on larvicidal activity. Zhong, Wanfang,Guo, Huifang,Zhong, Wanfang,Ding, Shaojun. 2015

作者其他论文 更多>>

微信小程序