Extraction, Purification and partial characterizations of Polysaccharides from Gracilaria lemaneiformis

文献类型： 外文期刊

第一作者： Yang, Xianqing

作者： Yang, Xianqing;Liu, Mingqiu;Qi, Bo;Li, Laihao;Deng, Jianchao;Hu, Xiao;Wu, Yanyan;Hao, Shuxian

作者机构：

关键词： extraction;purification;characterization;polysaccharides;Gracilaria lemaneiformis

期刊名称：CHEMICAL, MATERIAL AND METALLURGICAL ENGINEERING III, PTS 1-3

ISSN： 1022-6680

年卷期： 2014 年 881-883 卷

页码：

收录情况： SCI

摘要： In the present study, the crude polysaccharides were extracted from the microalgae Gracilaria lemaneiformis with ultrasonic-assisted extraction method and purified by DEAE-52 cellulose chromatography and Sephadex G-100 size-exclusion chromatography in that order. Two main fractions, GPC-1 and GPC-2, were obtained through the extraction and purification steps. Then partial characterizations, such as chemical composition, ultraviolet (UV) spectrum and infrared (IR) spectrum of the two fractions were analyzed in this study. The results indicated that the total sugar contents of GPC-1 and GPC-2 were 89.32% and 88.79%, and the protein contents of these two fractions were only 0.35% and 0.76%, compared with that of crude polysaccharides of 75.49% and 1.92%, respectively. Moreover, both the UV spectrum and the IR. spectrum demonstrated that the two fractions were typical purified polysaccharides. As a consequence, these investigated characterizations could be considered as a favorable evidence for the identification of polysaccharides from Gracilaria lemaneiformis.

分类号：

  • 相关文献

[1]Optimization of enzyme-assisted extraction of polysaccharides from alfalfa and its antioxidant activity. Wang, Shaopu,Dong, Xiaofang,Tong, Jianming.

[2]Extraction of Polysaccharides from Saccharomyces cerevisiae and its Immune Enhancement Activity. Wang, Hui,Dong, Pengcheng,Luo, Yongjiang,Cheng, Fusheng,Zhang, Xia. 2013

[3]Optimization of polysaccharides extraction from Tetrastigma hemsleyanum Diels et Gilg using response surface methodology. Shao, Qingsong,Zhao, Xiaofang,Deng, Yanming,Shen, Haijin,Fang, Hailing.

[4]Extraction and Purification of Astaxanthin from Shrimp Shell with Alkaline-organic-solvent Method. Liu, Hongchao,Wang, Guang,Yu, Heping,Zeng, Zongqiang,Li, Peng,Yang, Dan. 2012

[5]Purification, characterization, and complement fixation activity of acidic polysaccharides from Tuber sinoaestivum. Zhang, Bo,Zhou, Jie,He, Xiaolan,Ye, Lei,Zou, Jie,Wu, Chenguang,Peng, Weihong,Zhang, Bo,Zou, Jie,Wu, Chenguang,Zhang, Xiaoping,Li, Juan.

[6]Isolation, Purification, Antioxidant and Immunological Activities in Vitro of Polysaccharide from Acanthopanacis Senticosi. Chen Ruizhan,Dong Hang,Meng Fanlei,Liu Zhiqiang. 2011

[7]Purification, characterization and immunomodulatory activity of water extractable polysaccharides from the swollen culms of Zizania latifolia. Wang, Mingchun,Zhao, Shiwei,Zhu, Peilei,Nie, Chenzhipeng,Ma, Shuping,Wang, Naifu,Du, Xianfeng,Zhou, Yibin,Wang, Mingchun,Zhao, Shiwei,Zhu, Peilei,Nie, Chenzhipeng,Ma, Shuping,Wang, Naifu,Du, Xianfeng,Zhou, Yibin,Zhu, Peilei. 2018

[8]Tostadin, a novel antibacterial peptide from an antagonistic microorganism Brevibacillus brevis XDH. Liu, Xunli,Song, Zhen,Guo, Hui,Ju, Ruicheng,Zhao, Yuhua,Guo, Hui.

[9]Partial purification and characterization of polyphenol oxidase and peroxidase from chestnut kernel. Gong, Zhiqing,Cheng, Anwei,Wang, Wenliang,Gong, Zhiqing,Li, Dajing,Liu, Chunquan.

[10]Cloning, expression, purification, and characterization of an organophosphate-degrading enzyme in Escherichia coli. Wang, Jinbin,Chen, Dachao,Hu, Dongxiao,Su, Xuefeng,Tang, Xueming. 2013

[11]Purification and Characterization of a Thermostable Xylanase from Paenibacillus sp NF1 and its Application in Xylooligosaccharides Production. Zheng, Hong-chen,Pei, Hai-sheng,Yan, Zheng,Sun, Jun-she,Sun, Ming-zhe,Lu, Fu-ping,Meng, Ling-cai,Zhang, Xiu-qing,Zeng, Wen-hui,Zhang, Jing-sheng,Hu, Jin-rong,Sun, Jun-she. 2014

[12]Purification and properties of heat-stable extracellular protease from Pseudomonads fluorescens BJ-10. Zhang, Shuwen,Lv, Jiaping,Zhang, Shuwen,Lv, Jiaping. 2014

[13]Purification and biochemical characterization of a novel glutathione S-transferase of the silkworm, Bombyx mori. Hou, Cheng Xiang,Gui, Zhong Zheng,Fu, Zhi Qiang,Hou, Cheng Xiang,Gui, Zhong Zheng,Jin, Byung Rae. 2008

[14]Cloning, sequencing, and expression of a novel epoxide hydrolase gene from Rhodococcus opacus in Escherichia coli and characterization of enzyme. Liu, Zhiqiang,Li, Yin,Xu, Yingying,Ping, Lifeng,Zheng, Yuguo.

[15]Expression and Characterization of the Dictyoglomus thermophilum Rt46B.1 Xylanase Gene (xynB) in Bacillus subtilis. Zhang, Wei,Li, Guan,Zhang, Wei,Lou, Kai.

[16]Purification and Identification of an ACE Inhibitory Peptide from Walnut Protein. Liu, Meng,Du, Ming,Zhang, Yingchun,Xu, Weili,Zhang, Lanwei,Wang, Cong,Wang, Kejian.

[17]Purification and characterization of a novel laccase from the mushroom pleurotus nebrodensis. Wang, He-Xiang,Wang, He-Xiang,Ng, Tzi Bun,Zhang, Guo-Qing,Tian, Guo-Ting.

[18]Formation and early development of tetraspores of Gracilaria lemaneiformis (Gracilaria, Gracilariaceae) under laboratory conditions. Ye, NH,Wang, HX,Wang, GC. 2006

[19]Carbon dioxide fixation by the seaweed Gracilaria lemaneiformis in integrated multi-trophic aquaculture with the scallop Chlamys farreri in Sanggou Bay, China. Han, Tingting,Han, Tingting,Han, Tingting,Jiang, Zengjie,Fang, Jianguang,Zhang, Jihong,Mao, Yuze,Zou, Jian,Huang, Yao,Wang, Dongzhe,Huang, Yao,Wang, Dongzhe.

[20]Study of Ultrasonic-freeze-thaw-cycle Assisted Extraction of Polysaccharide and Phycobiliprotein from Gracilaria lemaneiformis. Liu, Mingqiu,Yang, Xianqing,Qi, Bo,Li, Laihao,Deng, Jianchao,Hu, Xiao. 2013

作者其他论文 更多>>

微信小程序