MOLECULAR CHARACTERIZATION, POLYMORPHISM AND EXPRESSION ANALYSIS OF SWAMP EEL MAJOR HISTOCOMPATIBILITY COMPLEX CLASS II B GENE, AFTER INFECTION BY AEROMONAS HYDROPHILIA

文献类型： 外文期刊

第一作者： Li, W.

作者： Li, W.;Sun, W. X.;Hu, J. F.;Hong, D. W.;Li, W.;Chen, S. L.

作者机构：

关键词： Major histocompatibility complex (MHC);cDNA;genomic structure;polymorphism;swamp eel (Monopterus albus)

期刊名称：JOURNAL OF ANIMAL AND PLANT SCIENCES （ 影响因子：0.49； 五年影响因子：0.686 ）

ISSN： 1018-7081

年卷期： 2014 年 24 卷 2 期

页码：

收录情况： SCI

摘要： Major histocompatibility complex (MHC) class II genes play a crucial role in the immune system of vertebrates. Here we report the cloning of full-length cDNA from the swamp eel (Monopterus albus) MHC class II B (MHC-DAB) gene. We described its genomic structure, molecular polymorphism and expression profiles. The full-length cDNA (Gen Bank accession No.:JQ236680) is 1339 bp, encoding a 249 amino acid peptide. The genomic sequence was identified to be 3066 bp in length, which contained six exons and five introns. Sequence comparison showed that the proposed amino acid sequence shared between 26.4 and 74.7% identity with other species. Thirty seven distinct alleles were isolated from sixty individuals. There were between two and five alleles per individual. The presence of five alleles in an individual suggested that there were at least three MHC IIB loci in the genome. At the protein-binding region d(N) was significantly greater than d(S), providing evidence of strong positive selection among swamp eel sequences. RT-PCR demonstrated high MHC-DAB expression in stomach, spleen, blood cell and liver, moderate expression in brain, skin and kidney, and low or negligible expression in heart, intestine and muscle. Great changes were observed in liver, spleen and intestine after challenged with pathogenic bacteria, Aeromonas hydrophilia.

分类号：

  • 相关文献

[1]Major histocompatibility complex class IIB allele polymorphism and its association with resistance/susceptibility to Vibrio anguillarum in Japanese flounder (Paralichthys olivaceus). Zhang, Y. X.,Chen, S. L.,Liu, Y. G.,Sha, Z. X.,Liu, Z. J.. 2006

[2]Isolation and characterization of polymorphic microsatellite loci from cDNA library of Scylla paramamosain. Ma, Chunyan,Ma, Hongyu,Ma, Lingbo,Jiang, Keji,Zhang, Fengying,Song, Wei. 2011

[3]Molecular polymorphism and expression analysis of MHC class II B gene from red sea bream (Chrysophrys major). Chen, SL,Zhang, YX,Xu, MY,Ji, XS,Yu, GC,Dong, CF.

[4]Cloning, characterization, and expression of a novel member of proteasomal subunits gene in turbot, Scophthalmus maximus. Zhang Bo,Song Wenping,Zheng Debin,Ma Chao,Xiao Guangxia,Wang Xianli. 2015

[5]Genetic variability of ten Chinese indigenous goats using MHC-linked microsatellite markers. Guang-Xin, E.,Huang, Yong-Fu,Zhao, Yong-Ju,Na, Ri-Su,Zhang, Jia-Hua,Wu, Xin,Ma, Yue-Hui,Gao, Hui-Jiang.

[6]Molecular Characterization, Gene Evolution, and Expression Analysis of the Fructose-1, 6-bisphosphate Aldolase (FBA) Gene Family in Wheat (Triticum aestivum L.). Lv, Geng-Yin,Guo, Xiao-Guang,Xie, Li-Ping,Xie, Chang-Gen,Zhang, Xiao-Hong,Yang, Yuan,Xiao, Lei,Tang, Yu-Ying,Guo, Ai-Guang,Xu, Hong,Xie, Chang-Gen,Guo, Ai-Guang,Xu, Hong,Pan, Xing-Lai. 2017

[7]Molecular cloning, expression and characterization of bovine UQCC and its association with body measurement traits. Liu, Yongfeng,Zan, Linsen,Zhao, Shuanping,Liu, Yongfeng,Huang, Honggang,Li, Yong,Tang, Zhonglin,Yang, Shulin,Li, Kui,Zan, Linsen. 2010

[8]Major histocompatibility complex class IIA and IIB genes of Nile tilapia Oreochromis niloticus: Genomic structure, molecular polymorphism and expression patterns. Pang, Ji-cai,Gao, Feng-ying,Lu, Mai-xin,Ye, Xing,Zhu, Hua-ping,Ke, Xiao-li,Gao, Feng-ying,Lu, Mai-xin,Ye, Xing,Zhu, Hua-ping,Ke, Xiao-li,Pang, Ji-cai. 2013

[9]Molecular cloning, genomic structure, polymorphism and expression analysis of major histocompatibility complex class IIA and IIB genes of half-smooth tongue sole (Cynoglossus semilaevis). Xu, Tian-jun,Chen, Song-lin,Ji, Xiang-shan,Sha, Zhen-xia,Xu, Tian-jun.

[10]Isolation and characterization of Toll-like receptor 21 and 22 genes from Nile tilapia, Oreochromis niloticus (Linnaeus). Pang, Ji-cai,Gao, Feng-ying,Zhao, Jin-liang,Pang, Ji-cai,Gao, Feng-ying,Wang, Miao,Lu, Mai-xin,Pang, Ji-cai,Gao, Feng-ying,Wang, Miao,Lu, Mai-xin.

[11]Genomic organization and promoter characterization of FcCTL, a C-type lectin-like protein in Fenneropenaeus chinensis. Lai, XiaoFang,Gao, Huan,Yan, Binlun,Lai, XiaoFang,Gao, Huan,Kong, Jie,Wang, QingYin,Meng, XianHong,Chen, Baiyao.

[12]Characterization of the porcine p65 subunit of NF-kappa B and its association with virus antibody levels. Cao, Jiyue,Li, Hegang,Ma, Guojian,Gui, Duan,Zhao, Shuanping,Wang, Pan,Ruan, Jinxue,Yang, Shulin,Li, Kui,He, Kongwang,Wang, Xuemin. 2011

[13]Characterization of porcine MMP-2 and its association with immune traits. Huang, Honggang,Zhao, Weimin,Tang, Zhonglin,Yang, Shulin,Cui, Wentao,Mu, Yulian,Chu, Mingxing,Li, Kui,Zhao, Weimin,Zhao, Shuhong,Wu, Zhengfang. 2009

[14]Structural Characterization and Association of Ovine Dickkopf-1 Gene with Wool Production and Quality Traits in Chinese Merino. Mu, Fang,Rong, Enguang,Jing, Yang,Ma, Guangwei,Yan, Xiaohong,Wang, Zhipeng,Li, Yumao,Li, Hui,Wang, Ning,Yang, Hua. 2017

[15]Interferon regulatory factor-2 in orange-spotted grouper (Epinephelus coioides): Gene, inductive expression pattern and subcellular localization. Shi, Yan,Yin, Jing-Kui,Zhu, Xin-Ping,Chen, Kun-Ci,Pan, De-Bo,Zhao, Zhe,Zhu, Xin-Ping,Gui, Jian-Fang.

[16]Molecular cloning and characterization of S-adenosylmethionine decarboxylase gene in rubber tree (Hevea brasiliensis). Zhao, Manman,Liu, Hui,Deng, Zhi,Chen, Jiangshu,Yang, Hong,Li, Dejun,Zhao, Manman,Li, Huiping,Xia, Zhihui.

[17]Genomic structure, characterization and expression analysis of a manganese superoxide dismutase from pearl oyster Pinctada fucata. Zhang, Dianchang,Cui, Shuge,Guo, Huayang,Jiang, Shigui,Zhang, Dianchang,Jiang, Shigui.

[18]Molecular characterization of the porcine STAT4 and STAT6 genes. Huang, Jing,Ma, Guojian,Zhu, Mengjin,Zhao, Shu-Hong,Huang, Jing,Ma, Guojian,Zhu, Mengjin,Zhao, Shu-Hong,Huang, Jing,Pan, Jianzhi,Zhang, Wenchang.

[19]Cloning, characterization and expression analysis of a cold shock domain family member YB-1 in turbot Scophthalmus maximus. Zhang, Yu-Xi,Zhang, Bo,Chen, Song-Lin,Wang, Xian-Li,Yang, Chang-Geng. 2012

[20]Molecular cloning and expression analysis of a hepcidin antimicrobial peptide gene from turbot (Scophthalmus maximus). Chen, Song-Lin,Li, Wei,Meng, Liang,Sha, Zhen-Xia,Wang, Zhi-Jian,Ren, Guo-Cheng. 2007

作者其他论文 更多>>

微信小程序