Transcriptome analysis of dormant tomonts of the marine fish ectoparasitic ciliate Cryptocaryon irritans under low temperature

文献类型： 外文期刊

第一作者： Yin, Fei

作者： Yin, Fei;Sun, Peng;Gao, Quanxin;Wang, Jiteng

作者机构：

关键词： Cryptocaryon irritans;Transcriptome;Dormant tomont;Cell division;Low temperature

期刊名称：PARASITES & VECTORS （ 影响因子：3.876； 五年影响因子：3.959 ）

ISSN： 1756-3305

年卷期： 2016 年 9 卷

页码：

收录情况： SCI

摘要： Background: Cryptocaryon irritans, a species of obligatory ciliate ectoparasite, can infect various species of marine teleost fish. Cryptocaryon irritans that fall to the seabed or aquarium bottom in winter can form "dormant tomonts" and wake up when the temperature rises the next year. Abundant studies and analyses on the dormant tomonts were carried out at the transcriptome level, in order to investigate the molecular mechanism of C. irritans tomonts entering the dormant state under low-temperature conditions. Methods: The paired-end sequencing strategy was used to better assemble the entire transcriptome de novo. All clean sequencing reads from each of the three libraries (Group A: untreated blank control; Group B: treated for 24 h at 12 degrees C; and Group C: developed for 24 h at 25 degrees C) were respectively mapped back to the transcriptome assembly using the bioinformatics software. Results: In this study, 25,695,034, 21,944,467, and 28,722,875 paired-end clean reads were obtained respectively from the three cDNA libraries of the C. irritans tomont by Illumina paired-end sequencing technology. A total of 25,925 unique transcript fragments (unigenes) were assembled, with an average length of 839 bp. Differentially expressed genes (DEGs) were scrutinized; in Group B/A pairwise comparison, 343 genes presented differential expression, including 265 up-regulated genes and 78 down-regulated genes in Group B; in Group C/A pairwise comparison, there were 567 DEGs, including 548 up-regulated genes and 19 down-regulated genes in Group C; and in Group B/C pairwise comparison, 185 genes showed differential expression, including 145 up-regulated genes and 40 down-regulated genes in Group B. Conclusions: This is the first transcriptomic analytical study of the C. irritans tomonts under low temperature. It can be concluded that most of the genes required for its cell survival under low temperature, or for cell entry into a deeper dormancy state were discovered, and that they might be considered as candidate genes to develop the diagnostic and control measures for cryptocaryoniasis.

分类号：

  • 相关文献

[1]Transcriptome and analysis on the complement and coagulation cascades pathway of large yellow croaker (Larimichthys crocea) to ciliate ectoparasite Cryptocaryon irritans infection. Yin, Fei,Gao, Quanxin,Tang, Baojun,Sun, Peng,Han, Kunhuang,Huang, Weiqing,Han, Kunhuang,Huang, Weiqing.

[2]Transcriptome profiling of litchi leaves in response to low temperature reveals candidate regulatory genes and key metabolic events during floral induction. Zhang, Hongna,Shen, Jiyuan,Chen, Houbin,Zhang, Hongna,Wei, Yongzan. 2017

[3]Growth, feed intake and immune responses of orange-spotted grouper (Epinephelus coioides) exposed to low infectious doses of ectoparasite (Cryptocaryon irritans). Yin, Fei,Sun, Peng,Shi, Zhao-Hong,Gao, Quan-Xin,Peng, Shi-Ming,Dan, Xue-Ming,Li, An-Xing. 2014

[4]Ultrastructural features of the tomont of Cryptocaryon irritans (Ciliophora: Prostomatea), a parasitic ciliate of marine fishes. Ma, Rui,Fan, Xinpeng,Ni, Bing,Gu, Fukang,Yin, Fei. 2017

[5]Effects of Cryptocaryon irritans infection on the survival, feeding, respiratory rate and ionic regulation of the marbled rockfish Sebastiscus marmoratus. Yin, Fei,Sun, Peng,Gao, Quanxin,Shi, Zhaohong,Peng, Shiming,Gong, Qiyang,Li, Yanwei,Li, Anxing,Dan, Xueming. 2014

[6]Immunological, ionic and biochemical responses in blood serum of the marine fish Trachinotus ovatus to poly-infection by Cryptocaryon irritans. Yin, Fei,Sun, Peng,Tang, Baojun,Dan, Xueming,Li, Anxing.

[7]Anti-parasitic effects of Leptomycin B isolated from Streptomyces sp CJK17 on marine fish ciliate Cryptocaryon irritans. Yin, Fei,Sun, Peng,Tang, Baojun,Gong, Hui,Ke, Qiaozhen,Li, Anxing.

[8]Molecular characterization and functional analysis of TRAF6 in orange-spotted grouper (Epinephelus coioides). Li, Yan-Wei,Li, Xia,Xiao, Xi-Xi,Li, An-Xing,Zhao, Fei,Luo, Xiao-Chun,Dan, Xue-Ming.

[9]Characterization of E3 ubiquitin ligase neuregulin receptor degradation protein-1 (Nrdp1) in the large yellow croaker (Larimichthys crocea) and its immune responses to Cryptocaryon irritans. Zhang, Dong Ling,Han, Fang,Xiao, Shi Jun,Chen, Jian,Wang, Zhi Yong,Yu, Da Hui,Li, Ming Yun.

[10]Placemat and rotational culturing: A novel method to control Cryptocaryon irritans infection by removing tomonts. Jiang, B.,Abdullahi, A. Y.,Li, G. Q.,Jiang, B.,Li, A. X.,Li, Y. W.,Dan, X. M.,Gong, H.,Ke, Q. Z..

[11]Stress, antioxidant defence and mucosal immune responses of the large yellow croaker Pseudosciaena crocea challenged with Cryptocaryon irritans. Yin, Fei,Gong, Hui,Ke, Qiaozhen,Li, Anxing.

[12]THE EFFECTS OF EXTRACELLULAR CALMODULIN ON CELL-WALL REGENERATION OF PROTOPLASTS AND CELL-DIVISION. SUN, DY,BIAN, YQ,ZHAO, BH,ZHAO, LY,YU, XM,SHENGJUN, D. 1995

[13]The regulatory factor X protein MoRfx1 is required for development and pathogenicity in the rice blast fungus Magnaporthe oryzae. Sun, Dandan,Cao, Huijuan,Shi, Yongkai,Huang, Pengyun,Lu, Jianping,Cao, Huijuan,Liu, Xiaohong,Lin, Fucheng,Dong, Bo. 2017

[14]Cell division and cell enlargement during potato tuber formation. Xu, X,Vreugdenhil, D,van Lammeren, AAM. 1998

[15]Influences of the Interference of gamma-tubulin Gene Expression on the Morphology and Microtubules of Ciliate Euplotes eurystomus. Sheng, Xin,Zeng, Hong,Zhang, Meng,Yun, Mi X.,Gu, Fu K.,Zeng, Hong,Yin, Fei.

[16]Characterization and expression profiling of cucumber kinesin genes during early fruit development: revealing the roles of kinesins in exponential cell production and enlargement in cucumber fruit. Yang, Xue Yong,Wang, Yan,Jiang, Wei Jie,Liu, Xiao Ling,Zhang, Xiao Meng,Yu, Hong Jun,Huang, San Wen,Liu, Guo Qin. 2013

[17]Genome-wide analysis of gene expression profiles during the kernel development of maize (Zea mays L.). Wang, Guoying,Liu, Xihui,Fu, Junjie,Li, Tingsong,Peng, Yunling,Wang, Guoying,Liu, Xihui,Fu, Junjie,Li, Tingsong,Peng, Yunling,Wang, Guoying,Gu, Dan,Liu, Wenxin,Wang, Jianhua. 2008

[18]Cloning, localization and expression analysis of two fw2.2-like genes in small- and large-fruited pear species. Tian Jia,Zeng Bin,Li Jiang,Luo Shu-ping,Li Xiu-gen,Wu Bin,Wu Bin.

[19]Regulation of fruit and seed response to heat and drought by sugars as nutrients and signals. Liu, Yong-Hua,Offler, Christina E.,Ruan, Yong-Ling,Liu, Yong-Hua. 2013

[20]Rice leaf inclination2, a VIN3-like protein, regulates leaf angle through modulating cell division of the collar. Zhao, Shu-Qing,Xue, Hong-Wei,Hu, Jiang,Guo, Long-Biao,Qian, Qian. 2010

作者其他论文 更多>>

微信小程序